Effect of hydrogen sulfide on myocardial protection in the setting of cardioplegia and cardiopulmonary bypass

Robert M. Osipov, Michael P. Robich, Jun Feng, Vincent Chan, Richard T. Clements, Ralph J. Deyo, Csaba Szabo, Frank W. Sellke

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

We investigated the impact of hydrogen sulfide (H2S) on myocardium in the setting of cold crystalloid cardioplegia and cardiopulmonary bypass (CP/CPB). Eighteen male Yorkshire pigs underwent 1 h CP/CPB followed by 2 h of reperfusion. Pigs received either: placebo (control, n=9), or H2S (as NaHS) as a bolusyinfusion (bolus/infusion, n=6), or as an infusion (infusion, n=6). The expression pattern of various myocardial effector pathways was investigated. Coronary microvascular relaxation to endothelium-dependent and -independent agonists was assessed. No differences in cardiac function were observed among groups. Endothelium-dependent microvascular relaxation to adenosine diphosphate was improved in the H2S bolusyinfusion group only (P<0.05). The expression of hemeoxygenase-1, phospho-heat shock proteins27 and phospho-p44/42 MAPK extracellular signal-regulated kinase were higher in H2S-treated groups (P<0.05). Phosphoendothelial nitric oxide synthase (P=0.08), phospho-B-cell lymphoma 2 (P=0.09), and phospho-Bad (P=0.06) all displayed a trend to be higher with H2S treatment. The expressions of apoptosis inducing factor and Bcl 2/adenovirus E1B 19 kDa-interacting protein were lower in HS treated groups (P<0.05). The microtubule-associated protein 1 light chain 3 ratio was lower in the infusion group vs. control animals 2 (P<0.05). There was a trend for lower phospho-mammalian target of rapamycin expression in the infusion group (P=0.07), whereas phosphorylation of p70S6K1 was higher with H2S-treatment (P=0.09). This study demonstrates that H2S-treatment may offer biochemical myocardial protection via attenuation of caspase-independent apoptosis and autophagy in the setting of CP/CPB.

Original languageEnglish (US)
Pages (from-to)506-512
Number of pages7
JournalInteractive Cardiovascular and Thoracic Surgery
Volume10
Issue number4
DOIs
StatePublished - Apr 2010
Externally publishedYes

Fingerprint

Hydrogen Sulfide
Induced Heart Arrest
Cardiopulmonary Bypass
Endothelium
Swine
Apoptosis Inducing Factor
Heme Oxygenase-1
Microtubule-Associated Proteins
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinase Kinases
Extracellular Signal-Regulated MAP Kinases
Autophagy
B-Cell Lymphoma
Sirolimus
Caspases
Adenoviridae
Nitric Oxide Synthase
Adenosine Diphosphate
Reperfusion
Shock

Keywords

  • Apoptosis
  • Cardiac function
  • Cardiopulmonary bypass
  • Myocardial protection/Cardioplegia

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Pulmonary and Respiratory Medicine
  • Surgery

Cite this

Effect of hydrogen sulfide on myocardial protection in the setting of cardioplegia and cardiopulmonary bypass. / Osipov, Robert M.; Robich, Michael P.; Feng, Jun; Chan, Vincent; Clements, Richard T.; Deyo, Ralph J.; Szabo, Csaba; Sellke, Frank W.

In: Interactive Cardiovascular and Thoracic Surgery, Vol. 10, No. 4, 04.2010, p. 506-512.

Research output: Contribution to journalArticle

Osipov, Robert M. ; Robich, Michael P. ; Feng, Jun ; Chan, Vincent ; Clements, Richard T. ; Deyo, Ralph J. ; Szabo, Csaba ; Sellke, Frank W. / Effect of hydrogen sulfide on myocardial protection in the setting of cardioplegia and cardiopulmonary bypass. In: Interactive Cardiovascular and Thoracic Surgery. 2010 ; Vol. 10, No. 4. pp. 506-512.
@article{5f8e162887e74d4395302af84fbe68c8,
title = "Effect of hydrogen sulfide on myocardial protection in the setting of cardioplegia and cardiopulmonary bypass",
abstract = "We investigated the impact of hydrogen sulfide (H2S) on myocardium in the setting of cold crystalloid cardioplegia and cardiopulmonary bypass (CP/CPB). Eighteen male Yorkshire pigs underwent 1 h CP/CPB followed by 2 h of reperfusion. Pigs received either: placebo (control, n=9), or H2S (as NaHS) as a bolusyinfusion (bolus/infusion, n=6), or as an infusion (infusion, n=6). The expression pattern of various myocardial effector pathways was investigated. Coronary microvascular relaxation to endothelium-dependent and -independent agonists was assessed. No differences in cardiac function were observed among groups. Endothelium-dependent microvascular relaxation to adenosine diphosphate was improved in the H2S bolusyinfusion group only (P<0.05). The expression of hemeoxygenase-1, phospho-heat shock proteins27 and phospho-p44/42 MAPK extracellular signal-regulated kinase were higher in H2S-treated groups (P<0.05). Phosphoendothelial nitric oxide synthase (P=0.08), phospho-B-cell lymphoma 2 (P=0.09), and phospho-Bad (P=0.06) all displayed a trend to be higher with H2S treatment. The expressions of apoptosis inducing factor and Bcl 2/adenovirus E1B 19 kDa-interacting protein were lower in HS treated groups (P<0.05). The microtubule-associated protein 1 light chain 3 ratio was lower in the infusion group vs. control animals 2 (P<0.05). There was a trend for lower phospho-mammalian target of rapamycin expression in the infusion group (P=0.07), whereas phosphorylation of p70S6K1 was higher with H2S-treatment (P=0.09). This study demonstrates that H2S-treatment may offer biochemical myocardial protection via attenuation of caspase-independent apoptosis and autophagy in the setting of CP/CPB.",
keywords = "Apoptosis, Cardiac function, Cardiopulmonary bypass, Myocardial protection/Cardioplegia",
author = "Osipov, {Robert M.} and Robich, {Michael P.} and Jun Feng and Vincent Chan and Clements, {Richard T.} and Deyo, {Ralph J.} and Csaba Szabo and Sellke, {Frank W.}",
year = "2010",
month = "4",
doi = "10.1510/icvts.2009.219535",
language = "English (US)",
volume = "10",
pages = "506--512",
journal = "Interactive Cardiovascular and Thoracic Surgery",
issn = "1569-9293",
publisher = "European Association for Cardio-Thoracic Surgery",
number = "4",

}

TY - JOUR

T1 - Effect of hydrogen sulfide on myocardial protection in the setting of cardioplegia and cardiopulmonary bypass

AU - Osipov, Robert M.

AU - Robich, Michael P.

AU - Feng, Jun

AU - Chan, Vincent

AU - Clements, Richard T.

AU - Deyo, Ralph J.

AU - Szabo, Csaba

AU - Sellke, Frank W.

PY - 2010/4

Y1 - 2010/4

N2 - We investigated the impact of hydrogen sulfide (H2S) on myocardium in the setting of cold crystalloid cardioplegia and cardiopulmonary bypass (CP/CPB). Eighteen male Yorkshire pigs underwent 1 h CP/CPB followed by 2 h of reperfusion. Pigs received either: placebo (control, n=9), or H2S (as NaHS) as a bolusyinfusion (bolus/infusion, n=6), or as an infusion (infusion, n=6). The expression pattern of various myocardial effector pathways was investigated. Coronary microvascular relaxation to endothelium-dependent and -independent agonists was assessed. No differences in cardiac function were observed among groups. Endothelium-dependent microvascular relaxation to adenosine diphosphate was improved in the H2S bolusyinfusion group only (P<0.05). The expression of hemeoxygenase-1, phospho-heat shock proteins27 and phospho-p44/42 MAPK extracellular signal-regulated kinase were higher in H2S-treated groups (P<0.05). Phosphoendothelial nitric oxide synthase (P=0.08), phospho-B-cell lymphoma 2 (P=0.09), and phospho-Bad (P=0.06) all displayed a trend to be higher with H2S treatment. The expressions of apoptosis inducing factor and Bcl 2/adenovirus E1B 19 kDa-interacting protein were lower in HS treated groups (P<0.05). The microtubule-associated protein 1 light chain 3 ratio was lower in the infusion group vs. control animals 2 (P<0.05). There was a trend for lower phospho-mammalian target of rapamycin expression in the infusion group (P=0.07), whereas phosphorylation of p70S6K1 was higher with H2S-treatment (P=0.09). This study demonstrates that H2S-treatment may offer biochemical myocardial protection via attenuation of caspase-independent apoptosis and autophagy in the setting of CP/CPB.

AB - We investigated the impact of hydrogen sulfide (H2S) on myocardium in the setting of cold crystalloid cardioplegia and cardiopulmonary bypass (CP/CPB). Eighteen male Yorkshire pigs underwent 1 h CP/CPB followed by 2 h of reperfusion. Pigs received either: placebo (control, n=9), or H2S (as NaHS) as a bolusyinfusion (bolus/infusion, n=6), or as an infusion (infusion, n=6). The expression pattern of various myocardial effector pathways was investigated. Coronary microvascular relaxation to endothelium-dependent and -independent agonists was assessed. No differences in cardiac function were observed among groups. Endothelium-dependent microvascular relaxation to adenosine diphosphate was improved in the H2S bolusyinfusion group only (P<0.05). The expression of hemeoxygenase-1, phospho-heat shock proteins27 and phospho-p44/42 MAPK extracellular signal-regulated kinase were higher in H2S-treated groups (P<0.05). Phosphoendothelial nitric oxide synthase (P=0.08), phospho-B-cell lymphoma 2 (P=0.09), and phospho-Bad (P=0.06) all displayed a trend to be higher with H2S treatment. The expressions of apoptosis inducing factor and Bcl 2/adenovirus E1B 19 kDa-interacting protein were lower in HS treated groups (P<0.05). The microtubule-associated protein 1 light chain 3 ratio was lower in the infusion group vs. control animals 2 (P<0.05). There was a trend for lower phospho-mammalian target of rapamycin expression in the infusion group (P=0.07), whereas phosphorylation of p70S6K1 was higher with H2S-treatment (P=0.09). This study demonstrates that H2S-treatment may offer biochemical myocardial protection via attenuation of caspase-independent apoptosis and autophagy in the setting of CP/CPB.

KW - Apoptosis

KW - Cardiac function

KW - Cardiopulmonary bypass

KW - Myocardial protection/Cardioplegia

UR - http://www.scopus.com/inward/record.url?scp=77950666590&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77950666590&partnerID=8YFLogxK

U2 - 10.1510/icvts.2009.219535

DO - 10.1510/icvts.2009.219535

M3 - Article

VL - 10

SP - 506

EP - 512

JO - Interactive Cardiovascular and Thoracic Surgery

JF - Interactive Cardiovascular and Thoracic Surgery

SN - 1569-9293

IS - 4

ER -