Effect of hydrogen sulfide on myocardial protection in the setting of cardioplegia and cardiopulmonary bypass

Robert M. Osipov, Michael P. Robich, Jun Feng, Vincent Chan, Richard T. Clements, Ralph J. Deyo, Csaba Szabo, Frank W. Sellke

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

We investigated the impact of hydrogen sulfide (H2S) on myocardium in the setting of cold crystalloid cardioplegia and cardiopulmonary bypass (CP/CPB). Eighteen male Yorkshire pigs underwent 1 h CP/CPB followed by 2 h of reperfusion. Pigs received either: placebo (control, n=9), or H2S (as NaHS) as a bolusyinfusion (bolus/infusion, n=6), or as an infusion (infusion, n=6). The expression pattern of various myocardial effector pathways was investigated. Coronary microvascular relaxation to endothelium-dependent and -independent agonists was assessed. No differences in cardiac function were observed among groups. Endothelium-dependent microvascular relaxation to adenosine diphosphate was improved in the H2S bolusyinfusion group only (P<0.05). The expression of hemeoxygenase-1, phospho-heat shock proteins27 and phospho-p44/42 MAPK extracellular signal-regulated kinase were higher in H2S-treated groups (P<0.05). Phosphoendothelial nitric oxide synthase (P=0.08), phospho-B-cell lymphoma 2 (P=0.09), and phospho-Bad (P=0.06) all displayed a trend to be higher with H2S treatment. The expressions of apoptosis inducing factor and Bcl 2/adenovirus E1B 19 kDa-interacting protein were lower in HS treated groups (P<0.05). The microtubule-associated protein 1 light chain 3 ratio was lower in the infusion group vs. control animals 2 (P<0.05). There was a trend for lower phospho-mammalian target of rapamycin expression in the infusion group (P=0.07), whereas phosphorylation of p70S6K1 was higher with H2S-treatment (P=0.09). This study demonstrates that H2S-treatment may offer biochemical myocardial protection via attenuation of caspase-independent apoptosis and autophagy in the setting of CP/CPB.

Original languageEnglish (US)
Pages (from-to)506-512
Number of pages7
JournalInteractive Cardiovascular and Thoracic Surgery
Volume10
Issue number4
DOIs
StatePublished - Apr 2010
Externally publishedYes

    Fingerprint

Keywords

  • Apoptosis
  • Cardiac function
  • Cardiopulmonary bypass
  • Myocardial protection/Cardioplegia

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Pulmonary and Respiratory Medicine
  • Surgery

Cite this

Osipov, R. M., Robich, M. P., Feng, J., Chan, V., Clements, R. T., Deyo, R. J., Szabo, C., & Sellke, F. W. (2010). Effect of hydrogen sulfide on myocardial protection in the setting of cardioplegia and cardiopulmonary bypass. Interactive Cardiovascular and Thoracic Surgery, 10(4), 506-512. https://doi.org/10.1510/icvts.2009.219535