Effect of hydroxychloroquine treatment on pro-inflammatory cytokines and disease activity in SLE patients: Data from LUMINA (LXXV), a multiethnic US cohort

R. Willis, A. M. Seif, G. McGwin, L. A. Martinez-Martinez, E. B. González, N. Dang, E. Papalardo, J. Liu, L. M. Vilá, J. D. Reveille, G. S. Alarcón, S. S. Pierangeli

Research output: Contribution to journalArticle

77 Scopus citations

Abstract

Objective: We sought to determine the effect of hydroxychloroquine therapy on the levels proinflammatory/prothrombotic markers and disease activity scores in patients with systemic lupus erythematosus (SLE) in a multiethnic, multi-center cohort (LUMINA). Methods: Plasma/serum samples from SLE patients (n=35) were evaluated at baseline and after hydroxychloroquine treatment. Disease activity was assessed using SLAM-R scores. Interferon (IFN)-α2, interleukin (IL)-1β, IL-6, IL-8, inducible protein (IP)-10, monocyte chemotactic protein-1, tumor necrosis factor (TNF)-α and soluble CD40 ligand (sCD40L) levels were determined by a multiplex immunoassay. Anticardiolipin antibodies were evaluated using ELISA assays. Thirty-two frequency-matched plasma/serum samples from healthy donors were used as controls. Results: Levels of IL-6, IP-10, sCD40L, IFN-α and TNF-α were significantly elevated in SLE patients versus controls. There was a positive but moderate correlation between SLAM-R scores at baseline and levels of IFN-α (p=0.0546). Hydroxychloroquine therapy resulted in a significant decrease in SLAM-R scores (p=0.0157), and the decrease in SLAM-R after hydroxychloroquine therapy strongly correlated with decreases in IFN-α (p=0.0087). Conclusions: Hydroxychloroquine therapy resulted in significant clinical improvement in SLE patients, which strongly correlated with reductions in IFN-α levels. This indicates an important role for the inhibition of endogenous TLR activation in the action of hydroxychloroquine in SLE and provides additional evidence for the importance of type I interferons in the pathogenesis of SLE. This study underscores the use of hydroxychloroquine in the treatment of SLE.

Original languageEnglish (US)
Pages (from-to)830-835
Number of pages6
JournalLupus
Volume21
Issue number8
DOIs
StatePublished - Jul 1 2012

Keywords

  • Lupus
  • biomarkers of inflammation
  • biomarkers of thrombosis
  • hydroxychloroquine

ASJC Scopus subject areas

  • Rheumatology

Fingerprint Dive into the research topics of 'Effect of hydroxychloroquine treatment on pro-inflammatory cytokines and disease activity in SLE patients: Data from LUMINA (LXXV), a multiethnic US cohort'. Together they form a unique fingerprint.

  • Cite this

    Willis, R., Seif, A. M., McGwin, G., Martinez-Martinez, L. A., González, E. B., Dang, N., Papalardo, E., Liu, J., Vilá, L. M., Reveille, J. D., Alarcón, G. S., & Pierangeli, S. S. (2012). Effect of hydroxychloroquine treatment on pro-inflammatory cytokines and disease activity in SLE patients: Data from LUMINA (LXXV), a multiethnic US cohort. Lupus, 21(8), 830-835. https://doi.org/10.1177/0961203312437270