Effect of inhibiting leukocyte integrin (CD18) and selectin (L-selectin) on susceptibility to infection with Pseudomonas aeruginosa

N. M. Garcia, William Mileski, P. Sikes, L. Atiles, E. Lightfoot, P. Lipsky, C. Baxter, E. J. DeMaria, W. Cioffi, D. E. Fry, M. A. Malangoni, R. V. Maier

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Leukocyte (WBC) adherence to endothelial cells has been implicated in the pathogenesis of microvascular injury. The process of leukocyte adherence is mediated by both the integrin and selectin families of molecules, and their interaction with specific endothelial ligands. Antibodies directed against the leukocyte integrin CD18 and L-selectin have been developed and functionally inhibit leukocyte adherence in models of inflammatory injury. We asked the question: Does inhibition of leukocyte adherence by administration of monoclonal antibody directed against either CD18, integrins (R15.7, R7.1) or against L-selectin (DREG 200) increase susceptibility to infection? New Zealand white rabbits were shaved and injected subcutaneously on their dorsum with Pseudomonas aeruginosa (ATCC 27853) at two sites each of 108 and 107 colony forming units. Animals were monitored with daily determination of weight, temperature, WBC counts, hematocrit, and killed at 1 week for determination of abscess formation. There were four blinded experimental groups: (1) Saline (2 mL/kg); (2) DREG 200 (2 mg/kg); (3) R7.1 (2 mg/kg); or (4) R15.7 (2 mg/kg). At the 107 and 108 injection sites the R15.7 group had an increased rate and size of abscess formation compared with controls. The R7.1 group had an increased rate at the 108 injection site. There was no significant difference in the percentage of the abscess formation or mean area between the controls and DREG 200-treated groups. We conclude that giving antibody to CD18 increased susceptibility to infection while giving antibody to L-selectin does not.

Original languageEnglish (US)
Pages (from-to)714-719
Number of pages6
JournalJournal of Trauma
Volume36
Issue number5
StatePublished - 1994
Externally publishedYes

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L-Selectin
Selectins
Integrins
Pseudomonas aeruginosa
Leukocytes
Abscess
Infection
Antibodies
Injections
Wounds and Injuries
Hematocrit
Stem Cells
Endothelial Cells
Monoclonal Antibodies
Rabbits
Ligands
Weights and Measures
Temperature

ASJC Scopus subject areas

  • Surgery

Cite this

Garcia, N. M., Mileski, W., Sikes, P., Atiles, L., Lightfoot, E., Lipsky, P., ... Maier, R. V. (1994). Effect of inhibiting leukocyte integrin (CD18) and selectin (L-selectin) on susceptibility to infection with Pseudomonas aeruginosa. Journal of Trauma, 36(5), 714-719.

Effect of inhibiting leukocyte integrin (CD18) and selectin (L-selectin) on susceptibility to infection with Pseudomonas aeruginosa. / Garcia, N. M.; Mileski, William; Sikes, P.; Atiles, L.; Lightfoot, E.; Lipsky, P.; Baxter, C.; DeMaria, E. J.; Cioffi, W.; Fry, D. E.; Malangoni, M. A.; Maier, R. V.

In: Journal of Trauma, Vol. 36, No. 5, 1994, p. 714-719.

Research output: Contribution to journalArticle

Garcia, NM, Mileski, W, Sikes, P, Atiles, L, Lightfoot, E, Lipsky, P, Baxter, C, DeMaria, EJ, Cioffi, W, Fry, DE, Malangoni, MA & Maier, RV 1994, 'Effect of inhibiting leukocyte integrin (CD18) and selectin (L-selectin) on susceptibility to infection with Pseudomonas aeruginosa', Journal of Trauma, vol. 36, no. 5, pp. 714-719.
Garcia, N. M. ; Mileski, William ; Sikes, P. ; Atiles, L. ; Lightfoot, E. ; Lipsky, P. ; Baxter, C. ; DeMaria, E. J. ; Cioffi, W. ; Fry, D. E. ; Malangoni, M. A. ; Maier, R. V. / Effect of inhibiting leukocyte integrin (CD18) and selectin (L-selectin) on susceptibility to infection with Pseudomonas aeruginosa. In: Journal of Trauma. 1994 ; Vol. 36, No. 5. pp. 714-719.
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AU - Garcia, N. M.

AU - Mileski, William

AU - Sikes, P.

AU - Atiles, L.

AU - Lightfoot, E.

AU - Lipsky, P.

AU - Baxter, C.

AU - DeMaria, E. J.

AU - Cioffi, W.

AU - Fry, D. E.

AU - Malangoni, M. A.

AU - Maier, R. V.

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