TY - JOUR
T1 - Effect of irradiation on human T-cell proliferation
T2 - Low dose irradiation stimulates mitogen-induced proliferation and function of the suppressor/cytotoxic T-cell subset
AU - Gualde, Norbert
AU - Goodwin, James S.
N1 - Funding Information:
’ This work was supported by Grants AGO1245 and RR00997 from the National Institutes of Health and by a grant from the Commission lnterprofessionelle Des Industries Laitieres (France), Laboratoire d Hematologie-Immunologic, Centre Hospitalier Universitaire Dupuytren, 2 rue Alexis Carrel, 8700 Limoges, France. * To whom requests for reprints should be addressed: University of New Mexico School of Medicine, Department of Medicine, Albuquerque, N.M. 87 13 I.
PY - 1984/4/1
Y1 - 1984/4/1
N2 - Unfractionated human T cells exposed to 10-50 rad of X irradiation incorporated less [3H]thymidine than nonirradiated T cells when subsequently cultured with PHA or Con A. The cytotoxic/suppressor T-cell subset, isolated as either OKT8(+) or OKT4(-) cells, demonstrated significantly enhanced [3H]thymidine incorporation in PHA- or Con A-stimulated cultures after exposure to 10-50 rad, compared to unirradiated cells, while the proliferation of the OKT4(+) helper/inducer subset was inhibited by low dose irradiation. It has been previously reported that ~30% of the cytotoxic/suppressor subset also stains with OKM1. When the cytotoxic/suppressor subset was further subdivided into OKT4(-), OKM1(+), and OKT4(-), OKM1(-) cells, proliferation of the OKT4(-), OKM1(+) population was inhibited by exposure to 25 rad while proliferation of the OKT4(-), OKM1(-) population was stimulated. The increase in proliferation of the cytotoxic/suppressor T-cell subset after low dose irradiation is paralleled by an increase in suppressor activity of these cells. T cells exposed to 25 rad and then cultured with Con A for 48 hr caused greater inhibition of IgG production when added to fresh autologous lymphocytes stimulated by pokeweed mitogen than did unirradiated cells. Thus, low dose irradiation enhances both the proliferation and function of the human suppressor T-cell subset.
AB - Unfractionated human T cells exposed to 10-50 rad of X irradiation incorporated less [3H]thymidine than nonirradiated T cells when subsequently cultured with PHA or Con A. The cytotoxic/suppressor T-cell subset, isolated as either OKT8(+) or OKT4(-) cells, demonstrated significantly enhanced [3H]thymidine incorporation in PHA- or Con A-stimulated cultures after exposure to 10-50 rad, compared to unirradiated cells, while the proliferation of the OKT4(+) helper/inducer subset was inhibited by low dose irradiation. It has been previously reported that ~30% of the cytotoxic/suppressor subset also stains with OKM1. When the cytotoxic/suppressor subset was further subdivided into OKT4(-), OKM1(+), and OKT4(-), OKM1(-) cells, proliferation of the OKT4(-), OKM1(+) population was inhibited by exposure to 25 rad while proliferation of the OKT4(-), OKM1(-) population was stimulated. The increase in proliferation of the cytotoxic/suppressor T-cell subset after low dose irradiation is paralleled by an increase in suppressor activity of these cells. T cells exposed to 25 rad and then cultured with Con A for 48 hr caused greater inhibition of IgG production when added to fresh autologous lymphocytes stimulated by pokeweed mitogen than did unirradiated cells. Thus, low dose irradiation enhances both the proliferation and function of the human suppressor T-cell subset.
UR - http://www.scopus.com/inward/record.url?scp=0021329620&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021329620&partnerID=8YFLogxK
U2 - 10.1016/0008-8749(84)90118-7
DO - 10.1016/0008-8749(84)90118-7
M3 - Article
C2 - 6231114
AN - SCOPUS:0021329620
SN - 0008-8749
VL - 84
SP - 439
EP - 445
JO - Cellular Immunology
JF - Cellular Immunology
IS - 2
ER -