TY - JOUR
T1 - Effect of isoprinosine on lymphocyte proliferation and natural killer cell activity following thermal injury
AU - Singh, Harbans
AU - Herndon, David N.
N1 - Funding Information:
This work was supported by a Grant from the Shriners Hospitals of North America. The authors wish to thank Mrs. Mary Goegar and Mrs. Nancy Berg for technical assistance.
PY - 1989
Y1 - 1989
N2 - The effect of in vivo administration of Isoprinosine (ISO) on, i) the proliferation of splenic lymphocytes in response to the T-cell mitogen, concanavalin-A (Con-A) and, ii) the natural killer (NK) cell cytotoxicity was studied following a full skin thickness burn injury in a rat model. Administration of ISO (100 mg/kg body wt/day) twice daily, resulted in significant augmentation of the proliferative responses of lymphocytes compared to non-treated burned animals, at 7 days post injury. However, it did not effect the lymphoproliferation at 14 days post injury, the time period at which a complete suppression of lymphocyte proliferation was observed in burned non-treated animals. Also, the proliferation of lymphocytes from normal nonburned animals was not affected by treatment with ISO. ISO treatment of the burned animals resulted in a significant increase in the NK cytotoxicity compared to non-treated burned animals. As with Con-A responses, ISO administered to control nonburned animals did not have any effect on NK cell cytotoxicity. Our studies thus indicate that ISO can be a potential immunomodulator of suppressed immune funtion following thermal injury, particularly in patients whose lymphocyte responses to T cell mitogen Con-A are not completely suppressed.
AB - The effect of in vivo administration of Isoprinosine (ISO) on, i) the proliferation of splenic lymphocytes in response to the T-cell mitogen, concanavalin-A (Con-A) and, ii) the natural killer (NK) cell cytotoxicity was studied following a full skin thickness burn injury in a rat model. Administration of ISO (100 mg/kg body wt/day) twice daily, resulted in significant augmentation of the proliferative responses of lymphocytes compared to non-treated burned animals, at 7 days post injury. However, it did not effect the lymphoproliferation at 14 days post injury, the time period at which a complete suppression of lymphocyte proliferation was observed in burned non-treated animals. Also, the proliferation of lymphocytes from normal nonburned animals was not affected by treatment with ISO. ISO treatment of the burned animals resulted in a significant increase in the NK cytotoxicity compared to non-treated burned animals. As with Con-A responses, ISO administered to control nonburned animals did not have any effect on NK cell cytotoxicity. Our studies thus indicate that ISO can be a potential immunomodulator of suppressed immune funtion following thermal injury, particularly in patients whose lymphocyte responses to T cell mitogen Con-A are not completely suppressed.
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U2 - 10.3109/08923978909005391
DO - 10.3109/08923978909005391
M3 - Article
C2 - 2483407
AN - SCOPUS:0024949671
SN - 0892-3973
VL - 11
SP - 631
EP - 644
JO - Immunopharmacology and Immunotoxicology
JF - Immunopharmacology and Immunotoxicology
IS - 4
ER -