Effect of miR-23 on Oxidant-induced injury in human retinal pigment epithelial cells

Haijiang Lin, Jinqiao Qian, Alexander C. Castillo, Bo Long, Kyle T. Keyes, Guanglin Chen, Yumei Ye

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Purpose. Micro(mi)RNAs negatively regulate a wide variety of genes through degradation or posttranslational inhibition of their target genes. The purpose of this study was to investigate the role of miR-23a in modulating RPE cell survival and gene expression in response to oxidative damage. Methods. The expression level of miR-23a was measured in macular retinal pigment epithelial (RPE) cells of donor eyes with aged-related macular degeneration (AMD) and age-matched normal eyes by using qRT-PCR. Cultured human ARPE-19 cells were transfected with miR-23a mimic or inhibitor. Cell viability was assessed by the MTT assay. Apoptosis was determined by incubating cells with hydrogen peroxide (H2O2) or t-butylhydroperoxide (tBH). Caspase-3 activity and DNA fragmentation were measured by enzyme-linked immunosorbent assays. The protein relevant to apoptosis, such as Fas expression level, was analyzed by Western blot analysis. Results. miR-23a expression was significantly downregulated in macular RPE cells from AMD eyes. H2O2-induced ARPE-19 cell death and apoptosis were increased by an miR-23a inhibitor and decreased by an miR-23a mimic. Computational analysis found a putative target site of miR-23a in the 3′UTR of Fas mRNA, which was verified by a luciferase reporter assay. Forced overexpression of miR-23a decreased H2O2 or tBH-induced Fas upregulation, and this effect was blocked by downregulation of miR-23a. Conclusions. The protection of RPE cells against oxidative damage is afforded by miR-23a through regulation of Fas, which may be a novel therapeutic target in retinal degenerative diseases.

Original languageEnglish (US)
Pages (from-to)6308-6314
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume52
Issue number9
DOIs
StatePublished - Aug 2011

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Retinal Pigments
Oxidants
Epithelial Cells
tert-Butylhydroperoxide
Wounds and Injuries
Macular Degeneration
Apoptosis
Cell Survival
Down-Regulation
Retinal Diseases
DNA Fragmentation
Luciferases
MicroRNAs
Caspase 3
Hydrogen Peroxide
Genes
Cell Death
Up-Regulation
Western Blotting
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience
  • Medicine(all)

Cite this

Effect of miR-23 on Oxidant-induced injury in human retinal pigment epithelial cells. / Lin, Haijiang; Qian, Jinqiao; Castillo, Alexander C.; Long, Bo; Keyes, Kyle T.; Chen, Guanglin; Ye, Yumei.

In: Investigative Ophthalmology and Visual Science, Vol. 52, No. 9, 08.2011, p. 6308-6314.

Research output: Contribution to journalArticle

Lin, Haijiang ; Qian, Jinqiao ; Castillo, Alexander C. ; Long, Bo ; Keyes, Kyle T. ; Chen, Guanglin ; Ye, Yumei. / Effect of miR-23 on Oxidant-induced injury in human retinal pigment epithelial cells. In: Investigative Ophthalmology and Visual Science. 2011 ; Vol. 52, No. 9. pp. 6308-6314.
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abstract = "Purpose. Micro(mi)RNAs negatively regulate a wide variety of genes through degradation or posttranslational inhibition of their target genes. The purpose of this study was to investigate the role of miR-23a in modulating RPE cell survival and gene expression in response to oxidative damage. Methods. The expression level of miR-23a was measured in macular retinal pigment epithelial (RPE) cells of donor eyes with aged-related macular degeneration (AMD) and age-matched normal eyes by using qRT-PCR. Cultured human ARPE-19 cells were transfected with miR-23a mimic or inhibitor. Cell viability was assessed by the MTT assay. Apoptosis was determined by incubating cells with hydrogen peroxide (H2O2) or t-butylhydroperoxide (tBH). Caspase-3 activity and DNA fragmentation were measured by enzyme-linked immunosorbent assays. The protein relevant to apoptosis, such as Fas expression level, was analyzed by Western blot analysis. Results. miR-23a expression was significantly downregulated in macular RPE cells from AMD eyes. H2O2-induced ARPE-19 cell death and apoptosis were increased by an miR-23a inhibitor and decreased by an miR-23a mimic. Computational analysis found a putative target site of miR-23a in the 3′UTR of Fas mRNA, which was verified by a luciferase reporter assay. Forced overexpression of miR-23a decreased H2O2 or tBH-induced Fas upregulation, and this effect was blocked by downregulation of miR-23a. Conclusions. The protection of RPE cells against oxidative damage is afforded by miR-23a through regulation of Fas, which may be a novel therapeutic target in retinal degenerative diseases.",
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