Effect of mucosal fluid from women with bacterial vaginosis on HIV trans-infection mediated by dendritic cells

Elizabeth P. St. John; M. Reza Zariffard; Jeffrey A. Martinson; Jose A. Simoes; Alan L. Landay; Gregory T. Spear

doi:10.1016/j.virol.2008.08.031

Effect of mucosal fluid from women with bacterial vaginosis on HIV trans-infection mediated by dendritic cells

Elizabeth P. St. John, M. Reza Zariffard, Jeffrey A. Martinson, Jose A. Simoes, Alan L. Landay, Gregory T. Spear

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Women with bacterial vaginosis (BV) have a higher risk of HIV transmission but the cause of risk is unknown. Dendritic cells (DC) are implicated in transmission of HIV and we previously observed that DC mature when exposed to mucosal fluid from women with BV. We hypothesized that maturation of DC by BV mucosal fluid would enhance DC-mediated trans-infection of HIV. Monocyte-derived DC (MDDC) were treated with mucosal fluid, incubated with HIV_Bal, and HIV trans-infection was evaluated. While LPS-treated MDDC increased HIV_Bal trans-infection, BV fluid reduced trans-infection. HIV_Bal DNA levels in MDDC were not affected by BV fluid or LPS but productive infection of MDDC was decreased by LPS and BV fluid. Mucosal fluid from women with BV does not increase MDDC-mediated trans-infection suggesting that BV does not increase HIV susceptibility by increasing DC-mediated trans-infection. However, indirect effects of DC maturation on HIV transmission cannot be ruled out.

Original language	English (US)
Pages (from-to)	22-27
Number of pages	6
Journal	Virology
Volume	385
Issue number	1
DOIs	https://doi.org/10.1016/j.virol.2008.08.031
State	Published - Mar 1 2009
Externally published	Yes

Keywords

Activation
Bacterial vaginosis
Cervical vaginal lavage
Dendritic cells
HIV
Maturation
MDDC
Toll-like receptors

ASJC Scopus subject areas

Virology

Access to Document

10.1016/j.virol.2008.08.031

Cite this

@article{35c0fbbe01e0430cb44c49a2a2752dd8,

title = "Effect of mucosal fluid from women with bacterial vaginosis on HIV trans-infection mediated by dendritic cells",

abstract = "Women with bacterial vaginosis (BV) have a higher risk of HIV transmission but the cause of risk is unknown. Dendritic cells (DC) are implicated in transmission of HIV and we previously observed that DC mature when exposed to mucosal fluid from women with BV. We hypothesized that maturation of DC by BV mucosal fluid would enhance DC-mediated trans-infection of HIV. Monocyte-derived DC (MDDC) were treated with mucosal fluid, incubated with HIVBal, and HIV trans-infection was evaluated. While LPS-treated MDDC increased HIVBal trans-infection, BV fluid reduced trans-infection. HIVBal DNA levels in MDDC were not affected by BV fluid or LPS but productive infection of MDDC was decreased by LPS and BV fluid. Mucosal fluid from women with BV does not increase MDDC-mediated trans-infection suggesting that BV does not increase HIV susceptibility by increasing DC-mediated trans-infection. However, indirect effects of DC maturation on HIV transmission cannot be ruled out.",

keywords = "Activation, Bacterial vaginosis, Cervical vaginal lavage, Dendritic cells, HIV, Maturation, MDDC, Toll-like receptors",

author = "{St. John}, {Elizabeth P.} and Zariffard, {M. Reza} and Martinson, {Jeffrey A.} and Simoes, {Jose A.} and Landay, {Alan L.} and Spear, {Gregory T.}",

year = "2009",

month = mar,

day = "1",

doi = "10.1016/j.virol.2008.08.031",

language = "English (US)",

volume = "385",

pages = "22--27",

journal = "Virology",

issn = "0042-6822",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Effect of mucosal fluid from women with bacterial vaginosis on HIV trans-infection mediated by dendritic cells

AU - St. John, Elizabeth P.

AU - Zariffard, M. Reza

AU - Martinson, Jeffrey A.

AU - Simoes, Jose A.

AU - Landay, Alan L.

AU - Spear, Gregory T.

PY - 2009/3/1

Y1 - 2009/3/1

N2 - Women with bacterial vaginosis (BV) have a higher risk of HIV transmission but the cause of risk is unknown. Dendritic cells (DC) are implicated in transmission of HIV and we previously observed that DC mature when exposed to mucosal fluid from women with BV. We hypothesized that maturation of DC by BV mucosal fluid would enhance DC-mediated trans-infection of HIV. Monocyte-derived DC (MDDC) were treated with mucosal fluid, incubated with HIVBal, and HIV trans-infection was evaluated. While LPS-treated MDDC increased HIVBal trans-infection, BV fluid reduced trans-infection. HIVBal DNA levels in MDDC were not affected by BV fluid or LPS but productive infection of MDDC was decreased by LPS and BV fluid. Mucosal fluid from women with BV does not increase MDDC-mediated trans-infection suggesting that BV does not increase HIV susceptibility by increasing DC-mediated trans-infection. However, indirect effects of DC maturation on HIV transmission cannot be ruled out.

AB - Women with bacterial vaginosis (BV) have a higher risk of HIV transmission but the cause of risk is unknown. Dendritic cells (DC) are implicated in transmission of HIV and we previously observed that DC mature when exposed to mucosal fluid from women with BV. We hypothesized that maturation of DC by BV mucosal fluid would enhance DC-mediated trans-infection of HIV. Monocyte-derived DC (MDDC) were treated with mucosal fluid, incubated with HIVBal, and HIV trans-infection was evaluated. While LPS-treated MDDC increased HIVBal trans-infection, BV fluid reduced trans-infection. HIVBal DNA levels in MDDC were not affected by BV fluid or LPS but productive infection of MDDC was decreased by LPS and BV fluid. Mucosal fluid from women with BV does not increase MDDC-mediated trans-infection suggesting that BV does not increase HIV susceptibility by increasing DC-mediated trans-infection. However, indirect effects of DC maturation on HIV transmission cannot be ruled out.

KW - Activation

KW - Bacterial vaginosis

KW - Cervical vaginal lavage

KW - Dendritic cells

KW - HIV

KW - Maturation

KW - MDDC

KW - Toll-like receptors

UR - http://www.scopus.com/inward/record.url?scp=60349094094&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=60349094094&partnerID=8YFLogxK

U2 - 10.1016/j.virol.2008.08.031

DO - 10.1016/j.virol.2008.08.031

M3 - Article

C2 - 19117586

AN - SCOPUS:60349094094

SN - 0042-6822

VL - 385

SP - 22

EP - 27

JO - Virology

JF - Virology

IS - 1

ER -

Effect of mucosal fluid from women with bacterial vaginosis on HIV trans-infection mediated by dendritic cells

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this