Effect of nitric oxide and carbon monoxide on uterine contractility during human and rat pregnancy

Monica Longo, Venu Jain, Yuri P. Vedernikov, George Saade, Linda Goodrum, Fabio Facchinetti, Robert E. Garfield

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

OBJECTIVE: We sought to study the effects of authentic nitric oxide and carbon monoxide on the contractile activity of pregnant human and rat myometrium. STUDY DESIGN: Strips were prepared from uterine biopsy specimens of 10 pregnant, nonlaboring women at term gestation undergoing cesarean delivery. In addition, rings were prepared from the uteri of pregnant rats at midterm (day 14) and at term (day 22) gestation (n = 10-12). The tissues were mounted in organ chambers filled with Krebs-Henseleit solution continuously aerated with 5% carbon dioxide in air (37°C, pH -7.4) for isometric tension recording. The effects of nitric oxide and carbon monoxide gases on spontaneous contractile activity were studied. Responses to hemin (hemoxygenase substrate), which produces endogenous carbon monoxide, were also examined. Responses to nitric oxide and carbon monoxide were also studied in aortic and tail artery rings from pregnant rats after contraction with phenylephrine. RESULTS: Nitric oxide significantly inhibited contractility of human myometrium at term (area under the concentration- response curve, 145.36 ± 30.02 vs 40.56 ± 22.81 in controls; P < .05) and rat myometrium at midterm gestation (264.23 ± 47.86 vs 121.82 ± 23.50; P < .05) but not at term. No statistically significant inhibition was induced in human or rat myometrium by carbon monoxide, whereas hemin significantly attenuated contractility in human myometrium at term and in rat myometrium at midterm gestation (P < .05). Nitric oxide, carbon monoxide, and hemin relaxed aortic and tail artery rings. CONCLUSIONS: Authentic nitric oxide inhibits rat uterine contractile activity at midterm gestation but not at term. However, nitric oxide inhibits human myometrium activity at term. Authentic carbon monoxide does not appear to modulate uterine contractility, whereas hemin may have some inhibitory properties.

Original languageEnglish (US)
Pages (from-to)981-988
Number of pages8
JournalAmerican Journal of Obstetrics and Gynecology
Volume181
Issue number4
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

Myometrium
Carbon Monoxide
Nitric Oxide
Hemin
Pregnancy
Human Activities
Tail
Arteries
Phenylephrine
Carbon Dioxide
Uterus
Pregnant Women
Gases
Air
Biopsy

Keywords

  • Carbon monoxide
  • Myometrium
  • Nitric oxide
  • Pregnancy

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Effect of nitric oxide and carbon monoxide on uterine contractility during human and rat pregnancy. / Longo, Monica; Jain, Venu; Vedernikov, Yuri P.; Saade, George; Goodrum, Linda; Facchinetti, Fabio; Garfield, Robert E.

In: American Journal of Obstetrics and Gynecology, Vol. 181, No. 4, 1999, p. 981-988.

Research output: Contribution to journalArticle

Longo, Monica ; Jain, Venu ; Vedernikov, Yuri P. ; Saade, George ; Goodrum, Linda ; Facchinetti, Fabio ; Garfield, Robert E. / Effect of nitric oxide and carbon monoxide on uterine contractility during human and rat pregnancy. In: American Journal of Obstetrics and Gynecology. 1999 ; Vol. 181, No. 4. pp. 981-988.
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T1 - Effect of nitric oxide and carbon monoxide on uterine contractility during human and rat pregnancy

AU - Longo, Monica

AU - Jain, Venu

AU - Vedernikov, Yuri P.

AU - Saade, George

AU - Goodrum, Linda

AU - Facchinetti, Fabio

AU - Garfield, Robert E.

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Y1 - 1999

N2 - OBJECTIVE: We sought to study the effects of authentic nitric oxide and carbon monoxide on the contractile activity of pregnant human and rat myometrium. STUDY DESIGN: Strips were prepared from uterine biopsy specimens of 10 pregnant, nonlaboring women at term gestation undergoing cesarean delivery. In addition, rings were prepared from the uteri of pregnant rats at midterm (day 14) and at term (day 22) gestation (n = 10-12). The tissues were mounted in organ chambers filled with Krebs-Henseleit solution continuously aerated with 5% carbon dioxide in air (37°C, pH -7.4) for isometric tension recording. The effects of nitric oxide and carbon monoxide gases on spontaneous contractile activity were studied. Responses to hemin (hemoxygenase substrate), which produces endogenous carbon monoxide, were also examined. Responses to nitric oxide and carbon monoxide were also studied in aortic and tail artery rings from pregnant rats after contraction with phenylephrine. RESULTS: Nitric oxide significantly inhibited contractility of human myometrium at term (area under the concentration- response curve, 145.36 ± 30.02 vs 40.56 ± 22.81 in controls; P < .05) and rat myometrium at midterm gestation (264.23 ± 47.86 vs 121.82 ± 23.50; P < .05) but not at term. No statistically significant inhibition was induced in human or rat myometrium by carbon monoxide, whereas hemin significantly attenuated contractility in human myometrium at term and in rat myometrium at midterm gestation (P < .05). Nitric oxide, carbon monoxide, and hemin relaxed aortic and tail artery rings. CONCLUSIONS: Authentic nitric oxide inhibits rat uterine contractile activity at midterm gestation but not at term. However, nitric oxide inhibits human myometrium activity at term. Authentic carbon monoxide does not appear to modulate uterine contractility, whereas hemin may have some inhibitory properties.

AB - OBJECTIVE: We sought to study the effects of authentic nitric oxide and carbon monoxide on the contractile activity of pregnant human and rat myometrium. STUDY DESIGN: Strips were prepared from uterine biopsy specimens of 10 pregnant, nonlaboring women at term gestation undergoing cesarean delivery. In addition, rings were prepared from the uteri of pregnant rats at midterm (day 14) and at term (day 22) gestation (n = 10-12). The tissues were mounted in organ chambers filled with Krebs-Henseleit solution continuously aerated with 5% carbon dioxide in air (37°C, pH -7.4) for isometric tension recording. The effects of nitric oxide and carbon monoxide gases on spontaneous contractile activity were studied. Responses to hemin (hemoxygenase substrate), which produces endogenous carbon monoxide, were also examined. Responses to nitric oxide and carbon monoxide were also studied in aortic and tail artery rings from pregnant rats after contraction with phenylephrine. RESULTS: Nitric oxide significantly inhibited contractility of human myometrium at term (area under the concentration- response curve, 145.36 ± 30.02 vs 40.56 ± 22.81 in controls; P < .05) and rat myometrium at midterm gestation (264.23 ± 47.86 vs 121.82 ± 23.50; P < .05) but not at term. No statistically significant inhibition was induced in human or rat myometrium by carbon monoxide, whereas hemin significantly attenuated contractility in human myometrium at term and in rat myometrium at midterm gestation (P < .05). Nitric oxide, carbon monoxide, and hemin relaxed aortic and tail artery rings. CONCLUSIONS: Authentic nitric oxide inhibits rat uterine contractile activity at midterm gestation but not at term. However, nitric oxide inhibits human myometrium activity at term. Authentic carbon monoxide does not appear to modulate uterine contractility, whereas hemin may have some inhibitory properties.

KW - Carbon monoxide

KW - Myometrium

KW - Nitric oxide

KW - Pregnancy

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