Effect of normal mosquito extracts upon arbovirus recoveries from mosquito pools

Thomas Ksiazek, J. L. Hardy, W. C. Reeves

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Titers of Turlock (TUR) and Hart Park (HP) viruses were reduced to undetectable levels when virus was mixed with a triturated suspension of uninfected (normal) 4th instar larvae of Culex tarsalis prior to plaque assay in cell culture. There was a linear relationship between the number of larvae in the pool and the titer of virus recoverable. Virus was undetectable when 1,000-10,000 PFU of either agent was added to pools that contained 25 or more larvae. Suspensions of up to 25 adult male or female Cx. tarsalis had little effect on detectable viral titers while pupal suspensions had an intermediate effect. The inhibitory effect of normal larval extracts on viral infectivity could be counteracted by use of polycations or a high pH buffer. A similar reduction in titer of TUR virus was observed with extracts of larvae of Aedes melanimon or Anopheles franciscanus. Larval extracts of Cx. tarsalis similarly reduced titers of California and St. Louis encephalitis viruses but not western equine encephalomyelitis virus. These findings may have significant bearing on the interpretation of transovarial transmission attempts in which pooled larvae are assayed for virus.

Original languageEnglish (US)
Pages (from-to)578-585
Number of pages8
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume34
Issue number3
StatePublished - 1985
Externally publishedYes

Fingerprint

Arboviruses
Culicidae
Larva
Viruses
Suspensions
Viral Load
St. Louis Encephalitis Viruses
Western Equine Encephalitis Viruses
Culex
Anopheles
Aedes
Buffers
Cell Culture Techniques

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Effect of normal mosquito extracts upon arbovirus recoveries from mosquito pools. / Ksiazek, Thomas; Hardy, J. L.; Reeves, W. C.

In: American Journal of Tropical Medicine and Hygiene, Vol. 34, No. 3, 1985, p. 578-585.

Research output: Contribution to journalArticle

@article{f6e53d7177c445f7b505e1544153d3b1,
title = "Effect of normal mosquito extracts upon arbovirus recoveries from mosquito pools",
abstract = "Titers of Turlock (TUR) and Hart Park (HP) viruses were reduced to undetectable levels when virus was mixed with a triturated suspension of uninfected (normal) 4th instar larvae of Culex tarsalis prior to plaque assay in cell culture. There was a linear relationship between the number of larvae in the pool and the titer of virus recoverable. Virus was undetectable when 1,000-10,000 PFU of either agent was added to pools that contained 25 or more larvae. Suspensions of up to 25 adult male or female Cx. tarsalis had little effect on detectable viral titers while pupal suspensions had an intermediate effect. The inhibitory effect of normal larval extracts on viral infectivity could be counteracted by use of polycations or a high pH buffer. A similar reduction in titer of TUR virus was observed with extracts of larvae of Aedes melanimon or Anopheles franciscanus. Larval extracts of Cx. tarsalis similarly reduced titers of California and St. Louis encephalitis viruses but not western equine encephalomyelitis virus. These findings may have significant bearing on the interpretation of transovarial transmission attempts in which pooled larvae are assayed for virus.",
author = "Thomas Ksiazek and Hardy, {J. L.} and Reeves, {W. C.}",
year = "1985",
language = "English (US)",
volume = "34",
pages = "578--585",
journal = "American Journal of Tropical Medicine and Hygiene",
issn = "0002-9637",
publisher = "American Society of Tropical Medicine and Hygiene",
number = "3",

}

TY - JOUR

T1 - Effect of normal mosquito extracts upon arbovirus recoveries from mosquito pools

AU - Ksiazek, Thomas

AU - Hardy, J. L.

AU - Reeves, W. C.

PY - 1985

Y1 - 1985

N2 - Titers of Turlock (TUR) and Hart Park (HP) viruses were reduced to undetectable levels when virus was mixed with a triturated suspension of uninfected (normal) 4th instar larvae of Culex tarsalis prior to plaque assay in cell culture. There was a linear relationship between the number of larvae in the pool and the titer of virus recoverable. Virus was undetectable when 1,000-10,000 PFU of either agent was added to pools that contained 25 or more larvae. Suspensions of up to 25 adult male or female Cx. tarsalis had little effect on detectable viral titers while pupal suspensions had an intermediate effect. The inhibitory effect of normal larval extracts on viral infectivity could be counteracted by use of polycations or a high pH buffer. A similar reduction in titer of TUR virus was observed with extracts of larvae of Aedes melanimon or Anopheles franciscanus. Larval extracts of Cx. tarsalis similarly reduced titers of California and St. Louis encephalitis viruses but not western equine encephalomyelitis virus. These findings may have significant bearing on the interpretation of transovarial transmission attempts in which pooled larvae are assayed for virus.

AB - Titers of Turlock (TUR) and Hart Park (HP) viruses were reduced to undetectable levels when virus was mixed with a triturated suspension of uninfected (normal) 4th instar larvae of Culex tarsalis prior to plaque assay in cell culture. There was a linear relationship between the number of larvae in the pool and the titer of virus recoverable. Virus was undetectable when 1,000-10,000 PFU of either agent was added to pools that contained 25 or more larvae. Suspensions of up to 25 adult male or female Cx. tarsalis had little effect on detectable viral titers while pupal suspensions had an intermediate effect. The inhibitory effect of normal larval extracts on viral infectivity could be counteracted by use of polycations or a high pH buffer. A similar reduction in titer of TUR virus was observed with extracts of larvae of Aedes melanimon or Anopheles franciscanus. Larval extracts of Cx. tarsalis similarly reduced titers of California and St. Louis encephalitis viruses but not western equine encephalomyelitis virus. These findings may have significant bearing on the interpretation of transovarial transmission attempts in which pooled larvae are assayed for virus.

UR - http://www.scopus.com/inward/record.url?scp=0021801211&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021801211&partnerID=8YFLogxK

M3 - Article

VL - 34

SP - 578

EP - 585

JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

SN - 0002-9637

IS - 3

ER -