Effect of normobaric hyperoxia on two indexes of synaptic function in fisher 344 rats

P. C. Bickford, K. Chadman, B. Williams, B. Shukitt-Hale, D. Holmes, Giulio Taglialatela, J. Joseph

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The physiological response of two central nervous system neurotransmitter receptors to oxidative stress was studied using the rat model of hyperoxia. We show that hyperoxia leads to a decline in the ability of isoproterenol (ISO) to augment GABAergic responses in cerebellar Purkinje neurons in vivo. This effect is reversed by the N-tert-butyl-α-phenylnitrone (PBN). We also show that hyperoxia produces a decline in the ability of oxotremorine (OXO) to stimulate dopamine (DA) release in striatal slices. This effect is accompanied by an increase in hydroxyl radical levels in the CNS reflected in an increase in 2,3-DHBA, suggesting that the change is the result of an increased level of oxidative stress. We also show a time dependent effect of hyperoxia on both β-adrenergic and muscarinic receptor function. We examined the interaction between age and hyperoxia exposure and found that in 12-month-old rats there is a decline in the baseline response prior to oxygen exposure that may interfere with observing a subsequent effect of hyperoxia. Differential effects were observed between the cerebellum and striatum with respect to the interaction of age and time of oxygen exposure. Overall, the data suggest that age and hyperoxia may be acting via a common mechanism because there was no synergistic effect of the two conditions.

Original languageEnglish (US)
Pages (from-to)817-824
Number of pages8
JournalFree Radical Biology and Medicine
Volume26
Issue number7-8
DOIs
StatePublished - Apr 1999

Fingerprint

Hyperoxia
Oxidative stress
Rats
Oxygen
Oxotremorine
Neurotransmitter Receptor
Neurology
Muscarinic Receptors
Isoproterenol
Hydroxyl Radical
Adrenergic Receptors
Neurons
Dopamine
Oxidative Stress
Corpus Striatum
Purkinje Cells
Cerebellum
Central Nervous System

Keywords

  • Aging
  • Cerebellum
  • Free radical
  • Hyperoxia
  • Oxidative stress
  • Striatum

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

Cite this

Effect of normobaric hyperoxia on two indexes of synaptic function in fisher 344 rats. / Bickford, P. C.; Chadman, K.; Williams, B.; Shukitt-Hale, B.; Holmes, D.; Taglialatela, Giulio; Joseph, J.

In: Free Radical Biology and Medicine, Vol. 26, No. 7-8, 04.1999, p. 817-824.

Research output: Contribution to journalArticle

Bickford, PC, Chadman, K, Williams, B, Shukitt-Hale, B, Holmes, D, Taglialatela, G & Joseph, J 1999, 'Effect of normobaric hyperoxia on two indexes of synaptic function in fisher 344 rats', Free Radical Biology and Medicine, vol. 26, no. 7-8, pp. 817-824. https://doi.org/10.1016/S0891-5849(98)00260-3
Bickford, P. C. ; Chadman, K. ; Williams, B. ; Shukitt-Hale, B. ; Holmes, D. ; Taglialatela, Giulio ; Joseph, J. / Effect of normobaric hyperoxia on two indexes of synaptic function in fisher 344 rats. In: Free Radical Biology and Medicine. 1999 ; Vol. 26, No. 7-8. pp. 817-824.
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