TY - JOUR
T1 - Effect of oral fat on plasma levels of neurotensin and neurotensin fragments in humans - Characterization by high-pressure liquid chromatography
AU - Draviam, Edwin J.
AU - Upp, James R.
AU - Greeley, George H.
AU - Townsend, Courtney M.
AU - Thompson, James C.
PY - 1990/2
Y1 - 1990/2
N2 - The effect of ingestion of fat (Lipomul 1 g/kg) on the circulating levels of neurotensin (NT1-3) and amino-terminal fragments (NT1-8, NT1-11) and carboxy-terminal fragment (NT8-13) of NT were investigated in six healthy male volunteers. NT and NT fragments were extracted from plasma collected at 0, 15, 30, and 60 min after ingestion of fat, and the plasma levels of NT1-13 and NT fragments were characterized using high-pressure liquid chromatography and radioimmunoassay techniques. Significant elevations of plasma levels of NT1-8, NT1-11, and NT1-13 were observed at 15, 30, and 60 min after fat ingestion. The maximum elevations were 273% for NT1-8, 234% for NT1-11, and 54% for NT1-13. NT8-13 levels failed to rise significantly when compared to basal levels. These findings indicate that both the aminoterminal and carboxyterminal fragments of NT are either released along with intact NT or are formed as metabolites from NT1-13 in response to ingestion of fat in man.
AB - The effect of ingestion of fat (Lipomul 1 g/kg) on the circulating levels of neurotensin (NT1-3) and amino-terminal fragments (NT1-8, NT1-11) and carboxy-terminal fragment (NT8-13) of NT were investigated in six healthy male volunteers. NT and NT fragments were extracted from plasma collected at 0, 15, 30, and 60 min after ingestion of fat, and the plasma levels of NT1-13 and NT fragments were characterized using high-pressure liquid chromatography and radioimmunoassay techniques. Significant elevations of plasma levels of NT1-8, NT1-11, and NT1-13 were observed at 15, 30, and 60 min after fat ingestion. The maximum elevations were 273% for NT1-8, 234% for NT1-11, and 54% for NT1-13. NT8-13 levels failed to rise significantly when compared to basal levels. These findings indicate that both the aminoterminal and carboxyterminal fragments of NT are either released along with intact NT or are formed as metabolites from NT1-13 in response to ingestion of fat in man.
KW - high-pressure liquid chromatography
KW - neurotensin
KW - peptide
KW - radioimmunoassay
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U2 - 10.1007/BF01536763
DO - 10.1007/BF01536763
M3 - Article
C2 - 2302977
AN - SCOPUS:0025181830
SN - 0163-2116
VL - 35
SP - 200
EP - 204
JO - Digestive Diseases and Sciences
JF - Digestive Diseases and Sciences
IS - 2
ER -