Effect of oxidative stress on plasma membrane fluidity of THP-1 induced macrophages

Carlos De La Haba, José R. Palacio, Paz Martínez, Antoni Morros

Research output: Contribution to journalArticlepeer-review

96 Scopus citations


Plasma membrane is one of the preferential targets of reactive oxygen species which cause lipid peroxidation. This process modifies membrane properties such as membrane fluidity, a very important physical feature known to modulate membrane protein localization and function. The aim of this study is to evaluate the effect of oxidative stress on plasma membrane fluidity regionalization of single living THP-1 macrophages. These cells were oxidized with H2O2 at different concentrations, and plasma membrane fluidity was analyzed by two-photon microscopy in combination with the environment-sensitive probe Laurdan. Results show a significant H 2O2 concentration dependent increase in the frequency of rigid lipid regions, mainly attributable to lipid rafts, at the expense of the intermediate fluidity regions. A novel statistical analysis evaluated changes in size and number of lipid raft domains under oxidative stress conditions, as lipid rafts are platforms aiding cell signaling and are thought to have relevant roles in macrophage functions. It is shown that H2O2 causes an increase in the number, but not the size, of raft domains. As macrophages are highly resistant to H2O2, these new raft domains might be involved in cell survival pathways.

Original languageEnglish (US)
Pages (from-to)357-364
Number of pages8
JournalBiochimica et Biophysica Acta - Biomembranes
Issue number2
StatePublished - Feb 2013
Externally publishedYes


  • Laurdan
  • Lipid raft
  • Macrophage
  • Membrane fluidity
  • Oxidative stress
  • Two-photon microscopy

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology


Dive into the research topics of 'Effect of oxidative stress on plasma membrane fluidity of THP-1 induced macrophages'. Together they form a unique fingerprint.

Cite this