Effect of retinoic acid on nerve growth factor receptors

Betty E. Haskell, Robert W. Stach, Karin Werrbach-Perez, J. Regino Perez-Polo

Research output: Contribution to journalArticlepeer-review

87 Scopus citations


Retinoic acid (RA), a naturally occurring metabolite of vitamin A, increased the number of receptors for nerve growth factor (NGF) in cultured human neuroblastoma cells (LA-N-1), as indicated by an immunofluorescence assay of cell surface receptors and by specific binding of 125I-NGF to solubilized receptors. Analysis of 125I-NGF binding showed that RA increased the number of both high affinity and low affinity receptors for NGF without affecting the equilibrium dissociation constants. Neurite outgrowth similar to that produced by NGF occurred following RA-treatment in LA-N-1 cells, in the SY5Y subclone of SK-N-SH human neuroblastoma cells and in explanted chick dorsal root ganglia (DRG). Whether morphological changes following RA treatment are directly related to the increase in NGF receptors is unknown. Data presented here are consistent with literature reports that RA modifies cell surface glycoproteins, including those that act as cell surface receptors for epidermal growth factor and insulin.

Original languageEnglish (US)
Pages (from-to)67-73
Number of pages7
JournalCell and Tissue Research
Issue number1
StatePublished - Jan 1987
Externally publishedYes


  • Cell culture
  • NGF
  • Neuroblastoma
  • Retinoic acid
  • receptors

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology


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