Effect of theophylline on substrate metabolism during exercise

Comasia A. Raguso, Andrew R. Coggan, Labros S. Sidossis, Amalia Gastaldelli, Robert R. Wolfe

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

We tested the hypothesis that adenosine is involved in regulating substrate metabolism during exercise. Seven trained cyclists were studied during 30 minutes of exercise at approximately 75% maximal oxygen uptake (V̇O2max). Lipid metabolism was evaluated by infusing [2H5]glycerol and [1-13C]palmitate, and glucose kinetics were evaluated by infusing [6,6- 2H]glucose. Fat and carbohydrate oxidation were also measured by indirect calorimetry. The same subjects performed two identical exercise tests, but in one trial theophylline, a potent adenosine receptor antagonist, was infused for 1 hour before and throughout exercise. Theophylline did not increase whole-body lipolysis (glycerol rate of appearance [R(a)]) or free fatty acid (FFA) release during exercise, but fat oxidation was lower than control values (9.5 ± 3.0 v 18.0 ± 4.2 μol · min-1 · kg-1, P < .01). Glucose R(a) was not affected by theophylline infusion, but glucose uptake was lower (31.6 ± 4.1 v 40.4 ± 5.0 μmol · min-1 · kg-1, P < .05) and glucose concentration was higher (6.4 ± 0.6 v 5.8 ± 0.4 mmol/L, P < .05) than in the control trial. Total carbohydrate oxidation (302.3 ± 26.2 v 265.5 ± 11.7 μmol · min-1 · kg-1, P < .06), estimated muscle glycogenolysis (270.7 ± 23.1 v 225.1 ± 9.7 μmol · min-1 · kg-1, p < .05), and plasma lactate concentration (7.9 ± 1.6 v 5.9 ± 1.1 mmol/L, P < .001) were also higher during the theophylline trial. These data suggest that adenosine may play a role in stimulating glucose uptake and restraining glycogenolysis but not in limiting lipolysis during exercise.

Original languageEnglish (US)
Pages (from-to)1153-1160
Number of pages8
JournalMetabolism: Clinical and Experimental
Volume45
Issue number9
DOIs
StatePublished - 1996
Externally publishedYes

Fingerprint

Theophylline
Glucose
Glycogenolysis
Lipolysis
Adenosine
Glycerol
Fats
Carbohydrates
Purinergic P1 Receptor Antagonists
Indirect Calorimetry
Palmitates
Exercise Test
Lipid Metabolism
Nonesterified Fatty Acids
Lactic Acid
Oxygen
Muscles

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Raguso, C. A., Coggan, A. R., Sidossis, L. S., Gastaldelli, A., & Wolfe, R. R. (1996). Effect of theophylline on substrate metabolism during exercise. Metabolism: Clinical and Experimental, 45(9), 1153-1160. https://doi.org/10.1016/S0026-0495(96)90016-5

Effect of theophylline on substrate metabolism during exercise. / Raguso, Comasia A.; Coggan, Andrew R.; Sidossis, Labros S.; Gastaldelli, Amalia; Wolfe, Robert R.

In: Metabolism: Clinical and Experimental, Vol. 45, No. 9, 1996, p. 1153-1160.

Research output: Contribution to journalArticle

Raguso, CA, Coggan, AR, Sidossis, LS, Gastaldelli, A & Wolfe, RR 1996, 'Effect of theophylline on substrate metabolism during exercise', Metabolism: Clinical and Experimental, vol. 45, no. 9, pp. 1153-1160. https://doi.org/10.1016/S0026-0495(96)90016-5
Raguso, Comasia A. ; Coggan, Andrew R. ; Sidossis, Labros S. ; Gastaldelli, Amalia ; Wolfe, Robert R. / Effect of theophylline on substrate metabolism during exercise. In: Metabolism: Clinical and Experimental. 1996 ; Vol. 45, No. 9. pp. 1153-1160.
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