Effect of transforming growth factor-β neutralization on survival and bacterial clearance in a murine model of Pseudomonas aeruginosa burn wound infection

Zhiyu Huang, Clifford Pereira, Tracy Toliver-Kinsky, Erle D. Murphey, Tushar K. Varma, Cheng Y. Lin, David Herndon, Edward R. Sherwood

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Transforming growth factor-β (TGF-β), a cytokine with anti-inflammatory properties, may contribute to postburn immunosuppression. This study was designed to determine whether neutralizing TGF-β in burned mice could improve resistance to infection. C57BL/6J mice received a 35% TBSA flame burn under isoflurane anesthesia. Four days after injury, mice were treated with TGF-β antibody or nonspecific IgG. On day 5 after burn injury, mice were inoculated with Pseudomonas aeruginosa at the burn wound site or received intraperitoneal injection with P. aeruginosa. Mice treated with anti-TGF-β exhibited significantly improved survival compared with mice treated with nonspecific IgG after challenge with P. aeruginosa at the burn wound site or after intraperitoneal injection of P. aeruginosa. In mice with burn wound infections, bacterial counts in burn wounds, blood, and lung were decreased in mice treated with anti-TGF-β compared with mice treated with control IgG. Bacterial counts in lung and blood after intraperitoneal challenge with P. aeruginosa also were significantly lower in burned mice treated with anti-TGF-β compared with those treated with nonspecific IgG. Our data suggest that neutralization of TGF-β at 4 days after burn injury in mice improves local and systemic clearance of P. aeruginosa and enhances survival after P. aeruginosa challenge.

Original languageEnglish (US)
Pages (from-to)682-687
Number of pages6
JournalJournal of Burn Care and Research
Volume27
Issue number5
DOIs
StatePublished - Sep 2006

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Transforming Growth Factors
Wound Infection
Pseudomonas aeruginosa
Wounds and Injuries
Immunoglobulin G
Bacterial Load
Intraperitoneal Injections
Lung
Isoflurane
Inbred C57BL Mouse
Immunosuppression
Anti-Inflammatory Agents
Anesthesia
Cytokines
Antibodies

ASJC Scopus subject areas

  • Emergency Medicine
  • Rehabilitation
  • Surgery

Cite this

Effect of transforming growth factor-β neutralization on survival and bacterial clearance in a murine model of Pseudomonas aeruginosa burn wound infection. / Huang, Zhiyu; Pereira, Clifford; Toliver-Kinsky, Tracy; Murphey, Erle D.; Varma, Tushar K.; Lin, Cheng Y.; Herndon, David; Sherwood, Edward R.

In: Journal of Burn Care and Research, Vol. 27, No. 5, 09.2006, p. 682-687.

Research output: Contribution to journalArticle

Huang, Zhiyu ; Pereira, Clifford ; Toliver-Kinsky, Tracy ; Murphey, Erle D. ; Varma, Tushar K. ; Lin, Cheng Y. ; Herndon, David ; Sherwood, Edward R. / Effect of transforming growth factor-β neutralization on survival and bacterial clearance in a murine model of Pseudomonas aeruginosa burn wound infection. In: Journal of Burn Care and Research. 2006 ; Vol. 27, No. 5. pp. 682-687.
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