Effect of uncoupling protein-1 expression on 3T3-L1 adipocyte gene expression

Fatih S. Senocak; Yaguang Si; Colby Moya; William K. Russell; David H. Russell; Kyongbum Lee; Arul Jayaraman

doi:10.1016/j.febslet.2007.11.064

Effect of uncoupling protein-1 expression on 3T3-L1 adipocyte gene expression

Fatih S. Senocak, Yaguang Si, Colby Moya, William K. Russell, David H. Russell, Kyongbum Lee, Arul Jayaraman

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article

8 Scopus citations

Abstract

The mitochondrial respiratory uncoupling protein 1 (UCP1) partially uncouples substrate oxidation and oxidative phosphorylation to promote the dissipation of cellular biochemical energy as heat in brown adipose tissue. We have recently shown that expression of UCP1 in 3T3-L1 white adipocytes reduces the accumulation of triglycerides. Here, we investigated the molecular basis underlying UCP1 expression in 3T3-L1 adipocytes. Gene expression data showed that forced UCP1 expression down-regulated several energy metabolism pathways; but ATP levels were constant. A metabolic flux analysis model was used to reflect the gene expression changes onto metabolic processes and concordance was observed in the down-regulation of energy consuming pathways. Our data suggest that adipocytes respond to long-term mitochondrial uncoupling by minimizing ATP utilization.

Original language	English (US)
Pages (from-to)	5865-5871
Number of pages	7
Journal	FEBS Letters
Volume	581
Issue number	30
DOIs	https://doi.org/10.1016/j.febslet.2007.11.064
State	Published - Dec 22 2007

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Keywords

Adipocytes
Energy metabolism
Uncoupling protein 1

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Cite this

Senocak, F. S., Si, Y., Moya, C., Russell, W. K., Russell, D. H., Lee, K., & Jayaraman, A. (2007). Effect of uncoupling protein-1 expression on 3T3-L1 adipocyte gene expression. FEBS Letters, 581(30), 5865-5871. https://doi.org/10.1016/j.febslet.2007.11.064

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10.1016/j.febslet.2007.11.064