Abstract
The mitochondrial respiratory uncoupling protein 1 (UCP1) partially uncouples substrate oxidation and oxidative phosphorylation to promote the dissipation of cellular biochemical energy as heat in brown adipose tissue. We have recently shown that expression of UCP1 in 3T3-L1 white adipocytes reduces the accumulation of triglycerides. Here, we investigated the molecular basis underlying UCP1 expression in 3T3-L1 adipocytes. Gene expression data showed that forced UCP1 expression down-regulated several energy metabolism pathways; but ATP levels were constant. A metabolic flux analysis model was used to reflect the gene expression changes onto metabolic processes and concordance was observed in the down-regulation of energy consuming pathways. Our data suggest that adipocytes respond to long-term mitochondrial uncoupling by minimizing ATP utilization.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 5865-5871 |
| Number of pages | 7 |
| Journal | FEBS Letters |
| Volume | 581 |
| Issue number | 30 |
| DOIs | |
| State | Published - Dec 22 2007 |
| Externally published | Yes |
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Keywords
- Adipocytes
- Energy metabolism
- Uncoupling protein 1
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Effect of uncoupling protein-1 expression on 3T3-L1 adipocyte gene expression. / Senocak, Fatih S.; Si, Yaguang; Moya, Colby; Russell, William; Russell, David H.; Lee, Kyongbum; Jayaraman, Arul.
In: FEBS Letters, Vol. 581, No. 30, 22.12.2007, p. 5865-5871.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of uncoupling protein-1 expression on 3T3-L1 adipocyte gene expression
AU - Senocak, Fatih S.
AU - Si, Yaguang
AU - Moya, Colby
AU - Russell, William
AU - Russell, David H.
AU - Lee, Kyongbum
AU - Jayaraman, Arul
PY - 2007/12/22
Y1 - 2007/12/22
N2 - The mitochondrial respiratory uncoupling protein 1 (UCP1) partially uncouples substrate oxidation and oxidative phosphorylation to promote the dissipation of cellular biochemical energy as heat in brown adipose tissue. We have recently shown that expression of UCP1 in 3T3-L1 white adipocytes reduces the accumulation of triglycerides. Here, we investigated the molecular basis underlying UCP1 expression in 3T3-L1 adipocytes. Gene expression data showed that forced UCP1 expression down-regulated several energy metabolism pathways; but ATP levels were constant. A metabolic flux analysis model was used to reflect the gene expression changes onto metabolic processes and concordance was observed in the down-regulation of energy consuming pathways. Our data suggest that adipocytes respond to long-term mitochondrial uncoupling by minimizing ATP utilization.
AB - The mitochondrial respiratory uncoupling protein 1 (UCP1) partially uncouples substrate oxidation and oxidative phosphorylation to promote the dissipation of cellular biochemical energy as heat in brown adipose tissue. We have recently shown that expression of UCP1 in 3T3-L1 white adipocytes reduces the accumulation of triglycerides. Here, we investigated the molecular basis underlying UCP1 expression in 3T3-L1 adipocytes. Gene expression data showed that forced UCP1 expression down-regulated several energy metabolism pathways; but ATP levels were constant. A metabolic flux analysis model was used to reflect the gene expression changes onto metabolic processes and concordance was observed in the down-regulation of energy consuming pathways. Our data suggest that adipocytes respond to long-term mitochondrial uncoupling by minimizing ATP utilization.
KW - Adipocytes
KW - Energy metabolism
KW - Uncoupling protein 1
UR - http://www.scopus.com/inward/record.url?scp=36849076271&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=36849076271&partnerID=8YFLogxK
U2 - 10.1016/j.febslet.2007.11.064
DO - 10.1016/j.febslet.2007.11.064
M3 - Article
C2 - 18061577
AN - SCOPUS:36849076271
VL - 581
SP - 5865
EP - 5871
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 30
ER -