Effect of undecylenic acid as a topical microbicide against genital herpes infection in mice and guinea pigs

Nigel Bourne, Jim Ireland, Lawrence R. Stanberry, David I. Bernstein

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

There is increasing interest in the use of topical microbicides to help prevent the spread of sexually transmitted diseases (STD). Undecylenic acid (UA), a monosaturated fatty acid, is the active ingredient in a number of over-the-counter (OTC) antifungal spray powders, that also exhibits in vitro antibacterial and antiviral activity, including herpes simplex virus (HSV) activity. We, therefore, evaluated UA as a topical microbicide against genital HSV infection using the murine and guinea pig models of genital herpes. Mice were administered a 20% solution of UA in polyethylene glycol (PEG) vehicle, vehicle alone or phosphate buffered saline (PBS) intravaginally immediately prior to vaginal challenge with HSV-2. Pre-treatment with UA decreased the number of mice that became infected (P < 0.001 vs. PBS or vehicle control), developed symptoms (P < 0.001) or died (P < 0.001). However, when treatment was extended to either 5 min prior to or after viral inoculation, protection was lost. Similar findings were found using the guinea pig model, where UA treatment completely prevented HSV-2 vaginal infection when given immediately prior to HSV-2 inoculation (P < 0.001 vs. PBS or vehicle control). Thus, UA, an approved OTC medication, provided significant protection against HSV disease and infection only when applied immediately before viral inoculation, indicating that better formulations were needed to extend the duration of protection.

Original languageEnglish (US)
Pages (from-to)139-144
Number of pages6
JournalAntiviral Research
Volume40
Issue number3
DOIs
StatePublished - Jan 1999
Externally publishedYes

Fingerprint

Herpes Genitalis
Local Anti-Infective Agents
Guinea Pigs
Human Herpesvirus 2
Infection
Phosphates
Virus Diseases
Simplexvirus
Sexually Transmitted Diseases
Powders
Antiviral Agents
undecylenic acid
Fatty Acids

Keywords

  • Genital herpes
  • Guinea pigs
  • Herpes simplex virus
  • Mice
  • Microbicides

ASJC Scopus subject areas

  • Virology
  • Pharmacology

Cite this

Effect of undecylenic acid as a topical microbicide against genital herpes infection in mice and guinea pigs. / Bourne, Nigel; Ireland, Jim; Stanberry, Lawrence R.; Bernstein, David I.

In: Antiviral Research, Vol. 40, No. 3, 01.1999, p. 139-144.

Research output: Contribution to journalArticle

Bourne, Nigel ; Ireland, Jim ; Stanberry, Lawrence R. ; Bernstein, David I. / Effect of undecylenic acid as a topical microbicide against genital herpes infection in mice and guinea pigs. In: Antiviral Research. 1999 ; Vol. 40, No. 3. pp. 139-144.
@article{4e94fd8d03074694b662c9a6588dff6e,
title = "Effect of undecylenic acid as a topical microbicide against genital herpes infection in mice and guinea pigs",
abstract = "There is increasing interest in the use of topical microbicides to help prevent the spread of sexually transmitted diseases (STD). Undecylenic acid (UA), a monosaturated fatty acid, is the active ingredient in a number of over-the-counter (OTC) antifungal spray powders, that also exhibits in vitro antibacterial and antiviral activity, including herpes simplex virus (HSV) activity. We, therefore, evaluated UA as a topical microbicide against genital HSV infection using the murine and guinea pig models of genital herpes. Mice were administered a 20{\%} solution of UA in polyethylene glycol (PEG) vehicle, vehicle alone or phosphate buffered saline (PBS) intravaginally immediately prior to vaginal challenge with HSV-2. Pre-treatment with UA decreased the number of mice that became infected (P < 0.001 vs. PBS or vehicle control), developed symptoms (P < 0.001) or died (P < 0.001). However, when treatment was extended to either 5 min prior to or after viral inoculation, protection was lost. Similar findings were found using the guinea pig model, where UA treatment completely prevented HSV-2 vaginal infection when given immediately prior to HSV-2 inoculation (P < 0.001 vs. PBS or vehicle control). Thus, UA, an approved OTC medication, provided significant protection against HSV disease and infection only when applied immediately before viral inoculation, indicating that better formulations were needed to extend the duration of protection.",
keywords = "Genital herpes, Guinea pigs, Herpes simplex virus, Mice, Microbicides",
author = "Nigel Bourne and Jim Ireland and Stanberry, {Lawrence R.} and Bernstein, {David I.}",
year = "1999",
month = "1",
doi = "10.1016/S0166-3542(98)00055-2",
language = "English (US)",
volume = "40",
pages = "139--144",
journal = "Antiviral Research",
issn = "0166-3542",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Effect of undecylenic acid as a topical microbicide against genital herpes infection in mice and guinea pigs

AU - Bourne, Nigel

AU - Ireland, Jim

AU - Stanberry, Lawrence R.

AU - Bernstein, David I.

PY - 1999/1

Y1 - 1999/1

N2 - There is increasing interest in the use of topical microbicides to help prevent the spread of sexually transmitted diseases (STD). Undecylenic acid (UA), a monosaturated fatty acid, is the active ingredient in a number of over-the-counter (OTC) antifungal spray powders, that also exhibits in vitro antibacterial and antiviral activity, including herpes simplex virus (HSV) activity. We, therefore, evaluated UA as a topical microbicide against genital HSV infection using the murine and guinea pig models of genital herpes. Mice were administered a 20% solution of UA in polyethylene glycol (PEG) vehicle, vehicle alone or phosphate buffered saline (PBS) intravaginally immediately prior to vaginal challenge with HSV-2. Pre-treatment with UA decreased the number of mice that became infected (P < 0.001 vs. PBS or vehicle control), developed symptoms (P < 0.001) or died (P < 0.001). However, when treatment was extended to either 5 min prior to or after viral inoculation, protection was lost. Similar findings were found using the guinea pig model, where UA treatment completely prevented HSV-2 vaginal infection when given immediately prior to HSV-2 inoculation (P < 0.001 vs. PBS or vehicle control). Thus, UA, an approved OTC medication, provided significant protection against HSV disease and infection only when applied immediately before viral inoculation, indicating that better formulations were needed to extend the duration of protection.

AB - There is increasing interest in the use of topical microbicides to help prevent the spread of sexually transmitted diseases (STD). Undecylenic acid (UA), a monosaturated fatty acid, is the active ingredient in a number of over-the-counter (OTC) antifungal spray powders, that also exhibits in vitro antibacterial and antiviral activity, including herpes simplex virus (HSV) activity. We, therefore, evaluated UA as a topical microbicide against genital HSV infection using the murine and guinea pig models of genital herpes. Mice were administered a 20% solution of UA in polyethylene glycol (PEG) vehicle, vehicle alone or phosphate buffered saline (PBS) intravaginally immediately prior to vaginal challenge with HSV-2. Pre-treatment with UA decreased the number of mice that became infected (P < 0.001 vs. PBS or vehicle control), developed symptoms (P < 0.001) or died (P < 0.001). However, when treatment was extended to either 5 min prior to or after viral inoculation, protection was lost. Similar findings were found using the guinea pig model, where UA treatment completely prevented HSV-2 vaginal infection when given immediately prior to HSV-2 inoculation (P < 0.001 vs. PBS or vehicle control). Thus, UA, an approved OTC medication, provided significant protection against HSV disease and infection only when applied immediately before viral inoculation, indicating that better formulations were needed to extend the duration of protection.

KW - Genital herpes

KW - Guinea pigs

KW - Herpes simplex virus

KW - Mice

KW - Microbicides

UR - http://www.scopus.com/inward/record.url?scp=0033008905&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033008905&partnerID=8YFLogxK

U2 - 10.1016/S0166-3542(98)00055-2

DO - 10.1016/S0166-3542(98)00055-2

M3 - Article

VL - 40

SP - 139

EP - 144

JO - Antiviral Research

JF - Antiviral Research

SN - 0166-3542

IS - 3

ER -