Effects induced by BMPS in cultures of human articular chondrocytes: Comparative studies

Susan Chubinskaya, Diane Segalite, Dmitriy Pikovsky, Arnavaz A. Hakimiyan, David C. Rueger

Research output: Contribution to journalArticlepeer-review

58 Scopus citations

Abstract

We compared anabolic and anti-catabolic activities of selected bone morphogenetic proteins (BMP-2, -4, -6, and -7) and cartilage-derived morphogenetic proteins (CDMP-1 and -2) in human normal adult articular chondrocytes. Ankle chondrocytes were cultured in alginate beads in the presence of 10% serum and treated with either growth factors only (each at 100ng/ml) or the combination of interleukin-1 (IL-1β) (0.1ng/ml) and BMPs. Chondrocyte metabolism was assessed by proteoglycan (PG) synthesis, content, DNA content, and cell survival. The results showed that BMP-2, -4, and -7 were more potent in stimulating PGs than other growth factors tested. The highest levels of PG synthesis were detected at day 9 in the presence of BMP-7. With regard to anti-catabolic properties, the effect depended upon treatment scheme (simultaneous or sequential). Under simultaneous cultures, BMP-2, -4, and -6 failed to counteract IL-1β induced inhibition of PG synthesis, while the CDMPs restored this parameter to serum control levels. Only BMP-7 showed consistent and pronounced anti-catabolic activity in either culture treatment scheme. None of the factors induced cell death or chondrocyte proliferation. In conclusion, the growth factors tested showed different levels of effects on human chondrocytes in culture, but only BMP-7 displayed both strong anabolic and anti-catabolic properties.

Original languageEnglish (US)
Pages (from-to)275-283
Number of pages9
JournalGrowth Factors
Volume26
Issue number5
DOIs
StatePublished - 2008
Externally publishedYes

Keywords

  • Bone morphogenetic proteins
  • Cartilage metabolism
  • Cartilage-derived morphogenetic proteins
  • Interleukin-1β
  • Proteoglycans

ASJC Scopus subject areas

  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

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