The in vitro addition of (-)-Δ9-tetrahydrocannabinol (Δ9-THC) resulted in a dose- responsive inhibition of the high-affinity specific synaptosomal uptake of both3H-tryptophan and3H-choline in mouse forebrain crude synaptosomal preparations. The approximate concentrations of Δ9-THC required to cause a 50% inhibition of the uptake of3H-tryptophan and3H-choline were 33 and 16 µM, respectively. Kinetic analysis showed that inhibition of both compounds were consistent with a noncompetitive mechanism. The pretreatment of mice with doses of 10, 30 or 100 mg/kg Δ9-THC had no effect on the subsequent in vitro synaptosomal uptake of either 3H-tryptophan or3H-choline into forebrain synaptosomes.
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