Effects of Δ9-THC on the synaptosomal uptake of 3H-tryptophan and 3H-choline

K. M. Johnson, W. L. Dewey

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The in vitro addition of (-)-Δ9-tetrahydrocannabinol (Δ9-THC) resulted in a dose-responsive inhibition of the high-affinity specific synaptosomal uptake of both 3H-tryptophan and 3H-choline in mouse forebrain crude synaptosomal preparations. The approximate concentrations of Δ9-THC required to cause a 50% inhibition of the uptake of 3H-tryptophan and 3H-choline were 33 and 16 μM, respectively. Kinetic analysis showed that inhibition of both compounds were consistent with a noncompetitive mechanism. The pretreatment of mice with doses of 10, 30 or 100 mg/kg Δ9-THC had no effect on the subsequent in vitro synaptosomal uptake of either 3H-tryptophan or 3H-choline into forebrain synaptosomes.

Original languageEnglish (US)
Pages (from-to)83-87
Number of pages5
JournalPharmacology
Volume17
Issue number2
StatePublished - 1978
Externally publishedYes

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Dronabinol
Choline
Tryptophan
Prosencephalon
Synaptosomes
In Vitro Techniques

ASJC Scopus subject areas

  • Pharmacology

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Effects of Δ9-THC on the synaptosomal uptake of 3H-tryptophan and 3H-choline. / Johnson, K. M.; Dewey, W. L.

In: Pharmacology, Vol. 17, No. 2, 1978, p. 83-87.

Research output: Contribution to journalArticle

Johnson, KM & Dewey, WL 1978, 'Effects of Δ9-THC on the synaptosomal uptake of 3H-tryptophan and 3H-choline', Pharmacology, vol. 17, no. 2, pp. 83-87.
Johnson, K. M. ; Dewey, W. L. / Effects of Δ9-THC on the synaptosomal uptake of 3H-tryptophan and 3H-choline. In: Pharmacology. 1978 ; Vol. 17, No. 2. pp. 83-87.
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