Effects of actinomycin D and ultraviolet and ionizing radiation on Pichinde virus

M. F. Carter, F. A. Murphy, J. P. Brunschwig, C. Noonan, W. E. Rawls

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Actinomycin D (0.05 μg/ml) suppresses the synthesis of ribosomal RNA of baby hamster kidney (BHK21) cells. The production of infectious Pichinde virus was enhanced in the presence of actinomycin D, although the production of virus particles was not substantially different from cultures inoculated in the absence of the drug. By prelabeling BHK21 cells with 3H-uridine and then allowing the virus to replicate in the presence of actinomycin D, it was possible to show that ribosomal RNA synthesized prior to infection was incorporated into the virion. A single hit kinetics of inactivation of Pichinde virus was observed with ultraviolet light, suggesting that the virus contains only a single copy of genome per virion. Comparison of the inactivation kinetics by gamma irradiation of Pichinde virus with Sindbis and rubella virus indicated that the radiosensitive genome of Pichinde virus was about 6 x 10 6 to 8 x 10 6 daltons. This value is greater than the 3.2 x 10 6 daltons which was estimated by biochemical analysis. One possible explanation considered is that the ribosomal RNA of host cell origin is functional and accounts for the differences in genome size estimated by the two methods.

Original languageEnglish (US)
Pages (from-to)33-38
Number of pages6
JournalJournal of Virology
Volume12
Issue number1
StatePublished - 1973

Fingerprint

Pichinde virus
actinomycin D
Dactinomycin
Ionizing Radiation
ionizing radiation
Ribosomal RNA
ultraviolet radiation
virion
Virion
ribosomal RNA
genome
inactivation
Rubella virus
Sindbis virus
Genome
Sindbis Virus
Viruses
kinetics
Genome Size
viruses

ASJC Scopus subject areas

  • Immunology

Cite this

Carter, M. F., Murphy, F. A., Brunschwig, J. P., Noonan, C., & Rawls, W. E. (1973). Effects of actinomycin D and ultraviolet and ionizing radiation on Pichinde virus. Journal of Virology, 12(1), 33-38.

Effects of actinomycin D and ultraviolet and ionizing radiation on Pichinde virus. / Carter, M. F.; Murphy, F. A.; Brunschwig, J. P.; Noonan, C.; Rawls, W. E.

In: Journal of Virology, Vol. 12, No. 1, 1973, p. 33-38.

Research output: Contribution to journalArticle

Carter, MF, Murphy, FA, Brunschwig, JP, Noonan, C & Rawls, WE 1973, 'Effects of actinomycin D and ultraviolet and ionizing radiation on Pichinde virus', Journal of Virology, vol. 12, no. 1, pp. 33-38.
Carter MF, Murphy FA, Brunschwig JP, Noonan C, Rawls WE. Effects of actinomycin D and ultraviolet and ionizing radiation on Pichinde virus. Journal of Virology. 1973;12(1):33-38.
Carter, M. F. ; Murphy, F. A. ; Brunschwig, J. P. ; Noonan, C. ; Rawls, W. E. / Effects of actinomycin D and ultraviolet and ionizing radiation on Pichinde virus. In: Journal of Virology. 1973 ; Vol. 12, No. 1. pp. 33-38.
@article{cfbc9f1ba0e64f0d9b82848e89abb4d5,
title = "Effects of actinomycin D and ultraviolet and ionizing radiation on Pichinde virus",
abstract = "Actinomycin D (0.05 μg/ml) suppresses the synthesis of ribosomal RNA of baby hamster kidney (BHK21) cells. The production of infectious Pichinde virus was enhanced in the presence of actinomycin D, although the production of virus particles was not substantially different from cultures inoculated in the absence of the drug. By prelabeling BHK21 cells with 3H-uridine and then allowing the virus to replicate in the presence of actinomycin D, it was possible to show that ribosomal RNA synthesized prior to infection was incorporated into the virion. A single hit kinetics of inactivation of Pichinde virus was observed with ultraviolet light, suggesting that the virus contains only a single copy of genome per virion. Comparison of the inactivation kinetics by gamma irradiation of Pichinde virus with Sindbis and rubella virus indicated that the radiosensitive genome of Pichinde virus was about 6 x 10 6 to 8 x 10 6 daltons. This value is greater than the 3.2 x 10 6 daltons which was estimated by biochemical analysis. One possible explanation considered is that the ribosomal RNA of host cell origin is functional and accounts for the differences in genome size estimated by the two methods.",
author = "Carter, {M. F.} and Murphy, {F. A.} and Brunschwig, {J. P.} and C. Noonan and Rawls, {W. E.}",
year = "1973",
language = "English (US)",
volume = "12",
pages = "33--38",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - Effects of actinomycin D and ultraviolet and ionizing radiation on Pichinde virus

AU - Carter, M. F.

AU - Murphy, F. A.

AU - Brunschwig, J. P.

AU - Noonan, C.

AU - Rawls, W. E.

PY - 1973

Y1 - 1973

N2 - Actinomycin D (0.05 μg/ml) suppresses the synthesis of ribosomal RNA of baby hamster kidney (BHK21) cells. The production of infectious Pichinde virus was enhanced in the presence of actinomycin D, although the production of virus particles was not substantially different from cultures inoculated in the absence of the drug. By prelabeling BHK21 cells with 3H-uridine and then allowing the virus to replicate in the presence of actinomycin D, it was possible to show that ribosomal RNA synthesized prior to infection was incorporated into the virion. A single hit kinetics of inactivation of Pichinde virus was observed with ultraviolet light, suggesting that the virus contains only a single copy of genome per virion. Comparison of the inactivation kinetics by gamma irradiation of Pichinde virus with Sindbis and rubella virus indicated that the radiosensitive genome of Pichinde virus was about 6 x 10 6 to 8 x 10 6 daltons. This value is greater than the 3.2 x 10 6 daltons which was estimated by biochemical analysis. One possible explanation considered is that the ribosomal RNA of host cell origin is functional and accounts for the differences in genome size estimated by the two methods.

AB - Actinomycin D (0.05 μg/ml) suppresses the synthesis of ribosomal RNA of baby hamster kidney (BHK21) cells. The production of infectious Pichinde virus was enhanced in the presence of actinomycin D, although the production of virus particles was not substantially different from cultures inoculated in the absence of the drug. By prelabeling BHK21 cells with 3H-uridine and then allowing the virus to replicate in the presence of actinomycin D, it was possible to show that ribosomal RNA synthesized prior to infection was incorporated into the virion. A single hit kinetics of inactivation of Pichinde virus was observed with ultraviolet light, suggesting that the virus contains only a single copy of genome per virion. Comparison of the inactivation kinetics by gamma irradiation of Pichinde virus with Sindbis and rubella virus indicated that the radiosensitive genome of Pichinde virus was about 6 x 10 6 to 8 x 10 6 daltons. This value is greater than the 3.2 x 10 6 daltons which was estimated by biochemical analysis. One possible explanation considered is that the ribosomal RNA of host cell origin is functional and accounts for the differences in genome size estimated by the two methods.

UR - http://www.scopus.com/inward/record.url?scp=0015807503&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015807503&partnerID=8YFLogxK

M3 - Article

VL - 12

SP - 33

EP - 38

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 1

ER -