Effects of amygdaloid kindling on NMDA receptor function and regulation

Susan M. Jones, Kenneth M. Johnson

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

These studies were conducted to determine whether amygdaloid kindling results in the long-term alteration of NMDA receptors which could explain the persistent reduction in seizure threshold seen in this phenomenon. NMDA-induced [3H]norepinephrine (NE) release, NMDA-sensitive l-[3H]glutamate binding, and NMDA and glycine-enhanced [3H]TCP binding were measured in brain tissue from kindled rats and nonstimulated control rats 3 to 6 weeks after the last seizure. There was no difference in the ability of NMDA to induce [3H]NE release from kindled or control slices of amygdala or hippocampus. There was also no difference in the ability of phencyclidine (PCP) or Mg2+ to inhibit [3H]NE release induced by 100 μM NMDA. Equilibrium saturation experiments of NMDA-sensitive l-[3H]glutamate binding revealed no differences in KD or Bmax values between control and kindled cortex, amygdala, and hippocampus. The Ki values for NMDA displacement of l-[3H]glutamate binding also did not differ in kindled tissue. NMDA-enhanced [3H]TCP binding was similar in cortex, amygdala, and hippocampus of kindled and control tissues. Finally, glycine-enhanced [3H]TCP binding was not different in control or kindled tissues. These studies suggest that the NMDA recognition site and the modulation of the NMDA receptor/ion channel complex by magnesium, PCP, and glycine are not altered several weeks after the last seizure. Even though NMDA-mediated electrophysiological responses are reportedly enhanced in kindled tissue at that time, the mechanism(s) underlying the enhancement remains to be determined.

Original languageEnglish (US)
Pages (from-to)52-60
Number of pages9
JournalExperimental Neurology
Volume106
Issue number1
DOIs
StatePublished - 1989

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N-Methylaspartate
N-Methyl-D-Aspartate Receptors
Amygdala
Glycine
Glutamic Acid
Hippocampus
Norepinephrine
Seizures
Phencyclidine
Ion Channels
Magnesium
Brain

ASJC Scopus subject areas

  • Neuroscience(all)
  • Neurology

Cite this

Effects of amygdaloid kindling on NMDA receptor function and regulation. / Jones, Susan M.; Johnson, Kenneth M.

In: Experimental Neurology, Vol. 106, No. 1, 1989, p. 52-60.

Research output: Contribution to journalArticle

Jones, Susan M. ; Johnson, Kenneth M. / Effects of amygdaloid kindling on NMDA receptor function and regulation. In: Experimental Neurology. 1989 ; Vol. 106, No. 1. pp. 52-60.
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