Abstract
Background: Recent studies have shown that interleukin-6 (IL-6) is involved in the systemic changes that are associated with infection and tissue injury and that there is a correlation between high levels of IL-6 and poor outcome during several pathologic conditions. Objective: The effects of antimurine IL-6 antibody on survival and host defense were studied in a clinically relevant model of infection. Methods: Balb/c mice were treated with 10 μg of antimurine interleukin-6, a nonspecific mouse IgG, or placebo 1 hour before they underwent bacterial challenge by gavage of 1010 Escherichia coli and burn injury. Survival and the extent of translocation of E coli were determined as well as the correlation between the IL-6 levels and survival times. Results: Survival after burn and gavage was 90% in animals treated with antimurine interleukin-6 vs 50% in animals treated with nonspecific IgG and 30% in saline-treated controls. A significant correlation between the levels of IL-6 and survival time was observed. Less translocation and better killing of bacteria were observed in the tissues of animals treated with antimurine interleukin-6. Conclusions: Interleukin-6 appears to play a major role in both the intensity of translocation of E coli from the intestine following burn injury and the host's ability to kill translocated organisms. Improved outcome was associated with a reduction of IL-6 levels by anti-IL-6 antibody.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1191-1197 |
| Number of pages | 7 |
| Journal | Archives of Surgery |
| Volume | 129 |
| Issue number | 11 |
| State | Published - 1994 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Surgery
Cite this
Effects of antimurine interleukin-6 on bacterial translocation during gut- derived sepsis. / Gennari, R.; Alexander, J. W.; Eaves-Pyles, Tonyia; Hartmann, S.; Ogle, C. K.; Moldawer, L. L.; McManus, A.; Chaudry, I.
In: Archives of Surgery, Vol. 129, No. 11, 1994, p. 1191-1197.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of antimurine interleukin-6 on bacterial translocation during gut- derived sepsis
AU - Gennari, R.
AU - Alexander, J. W.
AU - Eaves-Pyles, Tonyia
AU - Hartmann, S.
AU - Ogle, C. K.
AU - Moldawer, L. L.
AU - McManus, A.
AU - Chaudry, I.
PY - 1994
Y1 - 1994
N2 - Background: Recent studies have shown that interleukin-6 (IL-6) is involved in the systemic changes that are associated with infection and tissue injury and that there is a correlation between high levels of IL-6 and poor outcome during several pathologic conditions. Objective: The effects of antimurine IL-6 antibody on survival and host defense were studied in a clinically relevant model of infection. Methods: Balb/c mice were treated with 10 μg of antimurine interleukin-6, a nonspecific mouse IgG, or placebo 1 hour before they underwent bacterial challenge by gavage of 1010 Escherichia coli and burn injury. Survival and the extent of translocation of E coli were determined as well as the correlation between the IL-6 levels and survival times. Results: Survival after burn and gavage was 90% in animals treated with antimurine interleukin-6 vs 50% in animals treated with nonspecific IgG and 30% in saline-treated controls. A significant correlation between the levels of IL-6 and survival time was observed. Less translocation and better killing of bacteria were observed in the tissues of animals treated with antimurine interleukin-6. Conclusions: Interleukin-6 appears to play a major role in both the intensity of translocation of E coli from the intestine following burn injury and the host's ability to kill translocated organisms. Improved outcome was associated with a reduction of IL-6 levels by anti-IL-6 antibody.
AB - Background: Recent studies have shown that interleukin-6 (IL-6) is involved in the systemic changes that are associated with infection and tissue injury and that there is a correlation between high levels of IL-6 and poor outcome during several pathologic conditions. Objective: The effects of antimurine IL-6 antibody on survival and host defense were studied in a clinically relevant model of infection. Methods: Balb/c mice were treated with 10 μg of antimurine interleukin-6, a nonspecific mouse IgG, or placebo 1 hour before they underwent bacterial challenge by gavage of 1010 Escherichia coli and burn injury. Survival and the extent of translocation of E coli were determined as well as the correlation between the IL-6 levels and survival times. Results: Survival after burn and gavage was 90% in animals treated with antimurine interleukin-6 vs 50% in animals treated with nonspecific IgG and 30% in saline-treated controls. A significant correlation between the levels of IL-6 and survival time was observed. Less translocation and better killing of bacteria were observed in the tissues of animals treated with antimurine interleukin-6. Conclusions: Interleukin-6 appears to play a major role in both the intensity of translocation of E coli from the intestine following burn injury and the host's ability to kill translocated organisms. Improved outcome was associated with a reduction of IL-6 levels by anti-IL-6 antibody.
UR - http://www.scopus.com/inward/record.url?scp=0028104783&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028104783&partnerID=8YFLogxK
M3 - Article
C2 - 7979952
AN - SCOPUS:0028104783
VL - 129
SP - 1191
EP - 1197
JO - JAMA Surgery
JF - JAMA Surgery
SN - 2168-6254
IS - 11
ER -