Effects of antimurine interleukin-6 on bacterial translocation during gut- derived sepsis

R. Gennari, J. W. Alexander, Tonyia Eaves-Pyles, S. Hartmann, C. K. Ogle, L. L. Moldawer, A. McManus, I. Chaudry

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Background: Recent studies have shown that interleukin-6 (IL-6) is involved in the systemic changes that are associated with infection and tissue injury and that there is a correlation between high levels of IL-6 and poor outcome during several pathologic conditions. Objective: The effects of antimurine IL-6 antibody on survival and host defense were studied in a clinically relevant model of infection. Methods: Balb/c mice were treated with 10 μg of antimurine interleukin-6, a nonspecific mouse IgG, or placebo 1 hour before they underwent bacterial challenge by gavage of 1010 Escherichia coli and burn injury. Survival and the extent of translocation of E coli were determined as well as the correlation between the IL-6 levels and survival times. Results: Survival after burn and gavage was 90% in animals treated with antimurine interleukin-6 vs 50% in animals treated with nonspecific IgG and 30% in saline-treated controls. A significant correlation between the levels of IL-6 and survival time was observed. Less translocation and better killing of bacteria were observed in the tissues of animals treated with antimurine interleukin-6. Conclusions: Interleukin-6 appears to play a major role in both the intensity of translocation of E coli from the intestine following burn injury and the host's ability to kill translocated organisms. Improved outcome was associated with a reduction of IL-6 levels by anti-IL-6 antibody.

Original languageEnglish (US)
Pages (from-to)1191-1197
Number of pages7
JournalArchives of Surgery
Volume129
Issue number11
StatePublished - 1994
Externally publishedYes

Fingerprint

Bacterial Translocation
Interleukin-6
Sepsis
Escherichia coli
Wounds and Injuries
Immunoglobulin G
Antibodies
Infection
Intestines
Placebos

ASJC Scopus subject areas

  • Surgery

Cite this

Gennari, R., Alexander, J. W., Eaves-Pyles, T., Hartmann, S., Ogle, C. K., Moldawer, L. L., ... Chaudry, I. (1994). Effects of antimurine interleukin-6 on bacterial translocation during gut- derived sepsis. Archives of Surgery, 129(11), 1191-1197.

Effects of antimurine interleukin-6 on bacterial translocation during gut- derived sepsis. / Gennari, R.; Alexander, J. W.; Eaves-Pyles, Tonyia; Hartmann, S.; Ogle, C. K.; Moldawer, L. L.; McManus, A.; Chaudry, I.

In: Archives of Surgery, Vol. 129, No. 11, 1994, p. 1191-1197.

Research output: Contribution to journalArticle

Gennari, R, Alexander, JW, Eaves-Pyles, T, Hartmann, S, Ogle, CK, Moldawer, LL, McManus, A & Chaudry, I 1994, 'Effects of antimurine interleukin-6 on bacterial translocation during gut- derived sepsis', Archives of Surgery, vol. 129, no. 11, pp. 1191-1197.
Gennari R, Alexander JW, Eaves-Pyles T, Hartmann S, Ogle CK, Moldawer LL et al. Effects of antimurine interleukin-6 on bacterial translocation during gut- derived sepsis. Archives of Surgery. 1994;129(11):1191-1197.
Gennari, R. ; Alexander, J. W. ; Eaves-Pyles, Tonyia ; Hartmann, S. ; Ogle, C. K. ; Moldawer, L. L. ; McManus, A. ; Chaudry, I. / Effects of antimurine interleukin-6 on bacterial translocation during gut- derived sepsis. In: Archives of Surgery. 1994 ; Vol. 129, No. 11. pp. 1191-1197.
@article{a057b0f7a6054b10a4697c394974425e,
title = "Effects of antimurine interleukin-6 on bacterial translocation during gut- derived sepsis",
abstract = "Background: Recent studies have shown that interleukin-6 (IL-6) is involved in the systemic changes that are associated with infection and tissue injury and that there is a correlation between high levels of IL-6 and poor outcome during several pathologic conditions. Objective: The effects of antimurine IL-6 antibody on survival and host defense were studied in a clinically relevant model of infection. Methods: Balb/c mice were treated with 10 μg of antimurine interleukin-6, a nonspecific mouse IgG, or placebo 1 hour before they underwent bacterial challenge by gavage of 1010 Escherichia coli and burn injury. Survival and the extent of translocation of E coli were determined as well as the correlation between the IL-6 levels and survival times. Results: Survival after burn and gavage was 90{\%} in animals treated with antimurine interleukin-6 vs 50{\%} in animals treated with nonspecific IgG and 30{\%} in saline-treated controls. A significant correlation between the levels of IL-6 and survival time was observed. Less translocation and better killing of bacteria were observed in the tissues of animals treated with antimurine interleukin-6. Conclusions: Interleukin-6 appears to play a major role in both the intensity of translocation of E coli from the intestine following burn injury and the host's ability to kill translocated organisms. Improved outcome was associated with a reduction of IL-6 levels by anti-IL-6 antibody.",
author = "R. Gennari and Alexander, {J. W.} and Tonyia Eaves-Pyles and S. Hartmann and Ogle, {C. K.} and Moldawer, {L. L.} and A. McManus and I. Chaudry",
year = "1994",
language = "English (US)",
volume = "129",
pages = "1191--1197",
journal = "JAMA Surgery",
issn = "2168-6254",
publisher = "American Medical Association",
number = "11",

}

TY - JOUR

T1 - Effects of antimurine interleukin-6 on bacterial translocation during gut- derived sepsis

AU - Gennari, R.

AU - Alexander, J. W.

AU - Eaves-Pyles, Tonyia

AU - Hartmann, S.

AU - Ogle, C. K.

AU - Moldawer, L. L.

AU - McManus, A.

AU - Chaudry, I.

PY - 1994

Y1 - 1994

N2 - Background: Recent studies have shown that interleukin-6 (IL-6) is involved in the systemic changes that are associated with infection and tissue injury and that there is a correlation between high levels of IL-6 and poor outcome during several pathologic conditions. Objective: The effects of antimurine IL-6 antibody on survival and host defense were studied in a clinically relevant model of infection. Methods: Balb/c mice were treated with 10 μg of antimurine interleukin-6, a nonspecific mouse IgG, or placebo 1 hour before they underwent bacterial challenge by gavage of 1010 Escherichia coli and burn injury. Survival and the extent of translocation of E coli were determined as well as the correlation between the IL-6 levels and survival times. Results: Survival after burn and gavage was 90% in animals treated with antimurine interleukin-6 vs 50% in animals treated with nonspecific IgG and 30% in saline-treated controls. A significant correlation between the levels of IL-6 and survival time was observed. Less translocation and better killing of bacteria were observed in the tissues of animals treated with antimurine interleukin-6. Conclusions: Interleukin-6 appears to play a major role in both the intensity of translocation of E coli from the intestine following burn injury and the host's ability to kill translocated organisms. Improved outcome was associated with a reduction of IL-6 levels by anti-IL-6 antibody.

AB - Background: Recent studies have shown that interleukin-6 (IL-6) is involved in the systemic changes that are associated with infection and tissue injury and that there is a correlation between high levels of IL-6 and poor outcome during several pathologic conditions. Objective: The effects of antimurine IL-6 antibody on survival and host defense were studied in a clinically relevant model of infection. Methods: Balb/c mice were treated with 10 μg of antimurine interleukin-6, a nonspecific mouse IgG, or placebo 1 hour before they underwent bacterial challenge by gavage of 1010 Escherichia coli and burn injury. Survival and the extent of translocation of E coli were determined as well as the correlation between the IL-6 levels and survival times. Results: Survival after burn and gavage was 90% in animals treated with antimurine interleukin-6 vs 50% in animals treated with nonspecific IgG and 30% in saline-treated controls. A significant correlation between the levels of IL-6 and survival time was observed. Less translocation and better killing of bacteria were observed in the tissues of animals treated with antimurine interleukin-6. Conclusions: Interleukin-6 appears to play a major role in both the intensity of translocation of E coli from the intestine following burn injury and the host's ability to kill translocated organisms. Improved outcome was associated with a reduction of IL-6 levels by anti-IL-6 antibody.

UR - http://www.scopus.com/inward/record.url?scp=0028104783&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028104783&partnerID=8YFLogxK

M3 - Article

VL - 129

SP - 1191

EP - 1197

JO - JAMA Surgery

JF - JAMA Surgery

SN - 2168-6254

IS - 11

ER -