Effects of aromatase inhibitor (Fadrozole)-induced sex-reversal on gonadal differentiation and mRNA expression of P450arom, AMH and ERα in embryos and growth in posthatching quail

Naomi Koba, Masahiko Mori, Yonju Ha, Shusei Mizushima, Akira Tsukada, Noboru Saito, Tamao Ono, Kiyoshi Shimada

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The present study was conducted to investigate (1) the effective day of treatment with Fadrozole, nonsteroidal aromatase inhibitor (AI), during incubation for sex reversal, (2) the mRNA expression of aromatase (P450arom), anti-Müllerian hormone (AMH) and estrogen receptor α (ERα) in quail gonad and gonadal structures after the AI treatment before hatching and (3) the effects of AI on gonad growth, left-right asymmetry of the gonad and body weight gain between 1 to 7 weeks posthatch. (1) Females AI-treated at 0, 2 and 4 days of incubation had symmetrical gonads at hatch similar to normal males, whereas those treated at 6 and 8 days of incubation had asymmetrical gonads. (2) In females AI-treated at day 0 of incubation, the left gonad became an ovotestis at day 15 of incubation, displaying seminiferous tubules with testicular-like cords and ovarian follicle with ovum. In the control group, P450arom mRNA expression was significantly higher in females than males. However, AI-treated females showed 2 response-patterns of either higher or lower expression. The latter was similar to that of control males. No significant differences in levels of AMH and ERα mRNA between sexes either in control or AI-treated groups was observed. (3) A gradual increase in body weight of the control males and females without sex difference was observed up to 5 weeks of age. However, at 6-7 weeks the average weight of females was heavier than males each in control and treated group. These results indicate that shortly before day 6 of incubation P450arom mRNA expression, at least in a part, is important for asymmetrical formation of gonads in female quail. In AI-treated females, the high responder show similar level of P450arom mRNA in control males and the low responder do not respond to the AI. Body weight is controlled non-gonadally in quail.

Original languageEnglish (US)
Pages (from-to)116-124
Number of pages9
JournalJournal of Poultry Science
Volume45
Issue number2
DOIs
StatePublished - 2008
Externally publishedYes

Fingerprint

fadrozole
sex reversal
hormone receptors
quails
embryo (animal)
gonads
body weight
aromatase
estrogen receptors
seminiferous tubules
ovarian follicles
weight control
ova
gender differences

Keywords

  • Aromatase inhibitor
  • P450arom mRNA
  • Quail gonad
  • Sex-reversal

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Effects of aromatase inhibitor (Fadrozole)-induced sex-reversal on gonadal differentiation and mRNA expression of P450arom, AMH and ERα in embryos and growth in posthatching quail. / Koba, Naomi; Mori, Masahiko; Ha, Yonju; Mizushima, Shusei; Tsukada, Akira; Saito, Noboru; Ono, Tamao; Shimada, Kiyoshi.

In: Journal of Poultry Science, Vol. 45, No. 2, 2008, p. 116-124.

Research output: Contribution to journalArticle

Koba, Naomi ; Mori, Masahiko ; Ha, Yonju ; Mizushima, Shusei ; Tsukada, Akira ; Saito, Noboru ; Ono, Tamao ; Shimada, Kiyoshi. / Effects of aromatase inhibitor (Fadrozole)-induced sex-reversal on gonadal differentiation and mRNA expression of P450arom, AMH and ERα in embryos and growth in posthatching quail. In: Journal of Poultry Science. 2008 ; Vol. 45, No. 2. pp. 116-124.
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AU - Ha, Yonju

AU - Mizushima, Shusei

AU - Tsukada, Akira

AU - Saito, Noboru

AU - Ono, Tamao

AU - Shimada, Kiyoshi

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