Effects of artonin e on migration and invasion capabilities of human lung cancer cells

Kanok Plaibua, Varisa Pongrakhananon, Preedakorn Chunhacha, Boonchoo Sritularak, Pithi Chanvorachote

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Knowledge regarding substances that attenuate motility of cancer cells has gathered significant attention, as they benefit the development of novel anticancer strategies. The anti-migration and anti-invasion activities of artonin E, extracted from bark of Artocarpus gomezianus, were investigated in lung cancer cells in this study. Materials and Methods: Cytotoxicity and antiproliferative effects of artonin E were examined by 3- (4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Migration and invasion assays were performed on H460, H23, A549 and H292 human lung cancer cells. Cell morphology was determined by phalloidin-rhodamine staining. Motility-related proteins were investigated by western blotting. Results: Artonin E exhibited anti-migration and anti-invasion activities in H460 cells. Cell morphology revealed that treatment of the cells with non-toxic concentrations of artonin E resulted in a decrease of activated focal adhesion kinase (FAK), downstream protein kinase B (AKT) activation, and Cell division cycle-42 (CDC42), all of which were associated with the anti-motility effect of this compound. Artonin E inhibited invasion and migration of other lung cancer cells, namely H292, H23 and A549 cells. Conclusion: These results suggest that artonin E may be a promising candidate for anti-metastasis use.

Original languageEnglish (US)
Pages (from-to)3079-3088
Number of pages10
JournalAnticancer Research
Volume33
Issue number8
StatePublished - Aug 2013
Externally publishedYes

Fingerprint

Lung Neoplasms
Molecular Motor Proteins
Artocarpus
Focal Adhesion Protein-Tyrosine Kinases
Proto-Oncogene Proteins c-akt
Cell Movement
artonin E
Cell Cycle
Western Blotting
Staining and Labeling
Neoplasm Metastasis
Neoplasms

Keywords

  • A549
  • Artonin E
  • H23
  • H292 cells
  • H460
  • Invasion
  • Lung cancer cells
  • Metastasis
  • Migration

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Plaibua, K., Pongrakhananon, V., Chunhacha, P., Sritularak, B., & Chanvorachote, P. (2013). Effects of artonin e on migration and invasion capabilities of human lung cancer cells. Anticancer Research, 33(8), 3079-3088.

Effects of artonin e on migration and invasion capabilities of human lung cancer cells. / Plaibua, Kanok; Pongrakhananon, Varisa; Chunhacha, Preedakorn; Sritularak, Boonchoo; Chanvorachote, Pithi.

In: Anticancer Research, Vol. 33, No. 8, 08.2013, p. 3079-3088.

Research output: Contribution to journalArticle

Plaibua, K, Pongrakhananon, V, Chunhacha, P, Sritularak, B & Chanvorachote, P 2013, 'Effects of artonin e on migration and invasion capabilities of human lung cancer cells', Anticancer Research, vol. 33, no. 8, pp. 3079-3088.
Plaibua K, Pongrakhananon V, Chunhacha P, Sritularak B, Chanvorachote P. Effects of artonin e on migration and invasion capabilities of human lung cancer cells. Anticancer Research. 2013 Aug;33(8):3079-3088.
Plaibua, Kanok ; Pongrakhananon, Varisa ; Chunhacha, Preedakorn ; Sritularak, Boonchoo ; Chanvorachote, Pithi. / Effects of artonin e on migration and invasion capabilities of human lung cancer cells. In: Anticancer Research. 2013 ; Vol. 33, No. 8. pp. 3079-3088.
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