Effects of artonin e on migration and invasion capabilities of human lung cancer cells

Kanok Plaibua, Varisa Pongrakhananon, Preedakorn Chunhacha, Boonchoo Sritularak, Pithi Chanvorachote

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Background: Knowledge regarding substances that attenuate motility of cancer cells has gathered significant attention, as they benefit the development of novel anticancer strategies. The anti-migration and anti-invasion activities of artonin E, extracted from bark of Artocarpus gomezianus, were investigated in lung cancer cells in this study. Materials and Methods: Cytotoxicity and antiproliferative effects of artonin E were examined by 3- (4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Migration and invasion assays were performed on H460, H23, A549 and H292 human lung cancer cells. Cell morphology was determined by phalloidin-rhodamine staining. Motility-related proteins were investigated by western blotting. Results: Artonin E exhibited anti-migration and anti-invasion activities in H460 cells. Cell morphology revealed that treatment of the cells with non-toxic concentrations of artonin E resulted in a decrease of activated focal adhesion kinase (FAK), downstream protein kinase B (AKT) activation, and Cell division cycle-42 (CDC42), all of which were associated with the anti-motility effect of this compound. Artonin E inhibited invasion and migration of other lung cancer cells, namely H292, H23 and A549 cells. Conclusion: These results suggest that artonin E may be a promising candidate for anti-metastasis use.

Original languageEnglish (US)
Pages (from-to)3079-3088
Number of pages10
JournalAnticancer Research
Volume33
Issue number8
StatePublished - Aug 1 2013

Keywords

  • A549
  • Artonin E
  • H23
  • H292 cells
  • H460
  • Invasion
  • Lung cancer cells
  • Metastasis
  • Migration

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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