Effects of atractyloside and palmitoyl coenzyme A on calcium transport in cardiac mitochondria

G. K. Asimakis, Louis A. Sordahl

    Research output: Contribution to journalArticle

    50 Citations (Scopus)

    Abstract

    Palmitoyl CoA (PCoA) and the adenine translocase inhibitor atractyloside (ATR) appear to produce a similar effect in discharging accumulated calcium from cardiac mitochondria. Although mitochondrial respiration is stimulated upon addition of either PCoA or ATR to preparations preloaded with calcium, the effect is not the same as that produced by classical uncouplers. PCoA and ATR also do not interfere with respiration-supported calcium uptake by mitochondria. The presence of exogenous ATP can prevent the calcium discharging effects of PCoA or ATR. Carnitine will prevent the PCoA calcium discharging effect, but has no effect on ATR-induced discharge. It is suggested the PCoA may act at a site on or near the adenine translocase, perhaps through allosteric interaction, to produce an efflux of calcium from mitochondria. The results also suggest that the internal adenine nucleotide pool plays a significant role in mitochondrial calcium retention.

    Original languageEnglish (US)
    Pages (from-to)200-210
    Number of pages11
    JournalArchives of Biochemistry and Biophysics
    Volume179
    Issue number1
    DOIs
    StatePublished - 1977

    Fingerprint

    Atractyloside
    Palmitoyl Coenzyme A
    Mitochondria
    Calcium
    Adenine
    Respiration
    Adenine Nucleotides
    Carnitine
    Adenosine Triphosphate

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Molecular Biology

    Cite this

    Effects of atractyloside and palmitoyl coenzyme A on calcium transport in cardiac mitochondria. / Asimakis, G. K.; Sordahl, Louis A.

    In: Archives of Biochemistry and Biophysics, Vol. 179, No. 1, 1977, p. 200-210.

    Research output: Contribution to journalArticle

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