Effects of cyclosporin A and α-difluoromethylornithine on the growth of hamster pancreatic cancer in vitro

R. Saydjari, Courtney Townsend, S. C. Barranco, E. James, J. C. Thompson

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The growth and survival of hamster H2T pancreatic ductal adenocarcinoma in vitro are known to be significantly reduced by inhibitors of polyamine biosynthesis. α-Difluoromethylornithine (α-DFMO) is a specific and irreversible inhibitor of ornithine decarboxylase, the rate-limiting enzyme in polyamine biosynthesis. α-DFMO treatment inhibits the growth of H2T pancreatic cancer cells and decreases H2T cell survival in vitro and in vivo. In the present study the effects of cyclosporin A (CsA) were examined on growth, survival, and polyamine levels in H2T pancreatic ductal adenocarcinoma in vitro. CsA had inhibitory effects on H2T pancreatic cancer growth similar to those of α-DFMO; these effects were blocked by the addition of the polyamine putrescine. Polyamine levels were found to be significantly altered in cells treated with CsA and/or α-DFMO. The combination of CsA (8.3 x 104 mM) and α-DFMO (0.5 mM or 1.0 mM) inhibited H2T cell survival to a greater extent than either agent alone. These results suggest that CsA in combination with other agents that inhibit polyamine synthesis may be useful for the treatment of pancreatic cancer.

Original languageEnglish (US)
Pages (from-to)1087-1092
Number of pages6
JournalJournal of the National Cancer Institute
Volume77
Issue number5
StatePublished - 1986

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Eflornithine
Polyamines
Pancreatic Neoplasms
Cricetinae
Cyclosporine
Growth
Cell Survival
Adenocarcinoma
Putrescine
In Vitro Techniques
Enzymes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Effects of cyclosporin A and α-difluoromethylornithine on the growth of hamster pancreatic cancer in vitro. / Saydjari, R.; Townsend, Courtney; Barranco, S. C.; James, E.; Thompson, J. C.

In: Journal of the National Cancer Institute, Vol. 77, No. 5, 1986, p. 1087-1092.

Research output: Contribution to journalArticle

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