Effects of diabetes on oxidative and nitrosative stress in kidney mitochondria from aged rats

Rocío V. Pérez-Gallardo, Ruth Noriega-Cisneros, Edgar Esquivel-Gutiérrez, Elizabeth Calderón-Cortés, Christian Cortés-Rojo, Salvador Manzo-Avalos, Jesús Campos-García, Rafael Salgado-Garciglia, Rocío Montoya-Pérez, Istvan Boldogh, Alfredo Saavedra-Molina

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Diabetes mellitus (DM) is characterized by chronic hyperglycemia resulting from defects in the secretion and/or action of insulin. Diabetic nephropathy (DN) develops in diabetic patients and is characterized by a progressive deterioration of renal function. The mitochondrial electron transport chain (ETC) produces most of the reactive oxygen species (ROS) that are involved in diabetic nephropathy. Due to the high incidence of DM in the elderly, the aim of this study was to evaluate oxidative and nitrosative stress in kidney mitochondria from aged rats. We evaluated lipid peroxidation (LPO), nitric oxide (NO) production, S-nitrosylation profiles, glutathione levels, and glutathione reductase and aconitase activities under streptozotocin (STZ)-induced experimental diabetes in kidney mitochondria from aged rats. The results showed an increase in LPO, NO production, and S-nitrosylated proteins in rats with STZ-induced diabetes. A decrease in glutathione (GSH) levels and glutathione reductase (GR) and aconitase activities in the rats that received the STZ-induced diabetes treatment was also observed, when compared with the age-related controls. The data suggest that oxidative and nitrosative stresses promote mitochondrial oxidative dysfunction in the more advanced age rat kidney in STZ-induced diabetes.

Original languageEnglish (US)
Pages (from-to)511-518
Number of pages8
JournalJournal of Bioenergetics and Biomembranes
Volume46
Issue number6
DOIs
StatePublished - Dec 9 2014

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Mitochondria
Oxidative Stress
Experimental Diabetes Mellitus
Kidney
Aconitate Hydratase
Glutathione Reductase
Diabetic Nephropathies
Lipid Peroxidation
Glutathione
Diabetes Mellitus
Nitric Oxide
Protein S
Streptozocin
Electron Transport
Hyperglycemia
Reactive Oxygen Species
Insulin
Incidence
Therapeutics

Keywords

  • Aging
  • Diabetes
  • Glutathione
  • Kidney
  • Mitochondria
  • Nitric oxide
  • Oxidative stress

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Cite this

Pérez-Gallardo, R. V., Noriega-Cisneros, R., Esquivel-Gutiérrez, E., Calderón-Cortés, E., Cortés-Rojo, C., Manzo-Avalos, S., ... Saavedra-Molina, A. (2014). Effects of diabetes on oxidative and nitrosative stress in kidney mitochondria from aged rats. Journal of Bioenergetics and Biomembranes, 46(6), 511-518. https://doi.org/10.1007/s10863-014-9594-4

Effects of diabetes on oxidative and nitrosative stress in kidney mitochondria from aged rats. / Pérez-Gallardo, Rocío V.; Noriega-Cisneros, Ruth; Esquivel-Gutiérrez, Edgar; Calderón-Cortés, Elizabeth; Cortés-Rojo, Christian; Manzo-Avalos, Salvador; Campos-García, Jesús; Salgado-Garciglia, Rafael; Montoya-Pérez, Rocío; Boldogh, Istvan; Saavedra-Molina, Alfredo.

In: Journal of Bioenergetics and Biomembranes, Vol. 46, No. 6, 09.12.2014, p. 511-518.

Research output: Contribution to journalArticle

Pérez-Gallardo, RV, Noriega-Cisneros, R, Esquivel-Gutiérrez, E, Calderón-Cortés, E, Cortés-Rojo, C, Manzo-Avalos, S, Campos-García, J, Salgado-Garciglia, R, Montoya-Pérez, R, Boldogh, I & Saavedra-Molina, A 2014, 'Effects of diabetes on oxidative and nitrosative stress in kidney mitochondria from aged rats', Journal of Bioenergetics and Biomembranes, vol. 46, no. 6, pp. 511-518. https://doi.org/10.1007/s10863-014-9594-4
Pérez-Gallardo RV, Noriega-Cisneros R, Esquivel-Gutiérrez E, Calderón-Cortés E, Cortés-Rojo C, Manzo-Avalos S et al. Effects of diabetes on oxidative and nitrosative stress in kidney mitochondria from aged rats. Journal of Bioenergetics and Biomembranes. 2014 Dec 9;46(6):511-518. https://doi.org/10.1007/s10863-014-9594-4
Pérez-Gallardo, Rocío V. ; Noriega-Cisneros, Ruth ; Esquivel-Gutiérrez, Edgar ; Calderón-Cortés, Elizabeth ; Cortés-Rojo, Christian ; Manzo-Avalos, Salvador ; Campos-García, Jesús ; Salgado-Garciglia, Rafael ; Montoya-Pérez, Rocío ; Boldogh, Istvan ; Saavedra-Molina, Alfredo. / Effects of diabetes on oxidative and nitrosative stress in kidney mitochondria from aged rats. In: Journal of Bioenergetics and Biomembranes. 2014 ; Vol. 46, No. 6. pp. 511-518.
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