Effects of endotoxin on serum chemokines in man

Svenja Schinkel, C. Schinkel, V. Pollard, R. Garofallo, H. Heberle, P. Reisner, J. Papaconstantinou, D. N. Herndon

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Aim: Endotoxin is known to be a primary initiator of sepsis and septic shock. Migration of immunocompetent cells due to chemotactic attraction plays a central role in the initiation of the immune response. Two major groups of chemokines can be distinguished: C-x-C chemokines like Interleukin-8 attract mainly neutrophils, C-C chemokines (e.g. RANTES) attract monocytes and T-cells. The aim of this study was to get further insight into chemokine profiles after a single endotoxin bolus in man. Materials and Methods: We investigated the effect of systemically administered endotoxin (4ng/kg BW i.v.) in 8 healthy volunteers. Clinical data (heart rate, mean arterial pressure, temperature), serum levels of IL-8, and RANTES, as well as white blood cell count were obtained before and hourly for five hours after endotoxin administration. Results: Heart rate and MAP showed significant changes (p<0.05) after 2-3 hours. All volunteers presented with low-grade fever after 2 hours. WBC was elevated 43% and 63% after 4 and 5 hours, respectively. Both chemokines were significantly different from baseline two hours after endotoxin challenge: While IL-8 was significantly increased RANTES serum levels were diminished. Conclusion: From our data we conclude that this endotoxin model was effective to mimic the clinical appearance of sepsis. Chemokines like IL-8 and RANTES are integrated in the early immune response to endotoxin challenge in man.

Original languageEnglish (US)
Pages (from-to)76-80
Number of pages5
JournalEuropean Journal of Medical Research
Volume10
Issue number2
StatePublished - Feb 28 2005

Keywords

  • Chemokines
  • Endotoxin
  • IL-8
  • RANTES
  • Sepsis
  • Volunteer study

ASJC Scopus subject areas

  • Medicine(all)

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    Schinkel, S., Schinkel, C., Pollard, V., Garofallo, R., Heberle, H., Reisner, P., Papaconstantinou, J., & Herndon, D. N. (2005). Effects of endotoxin on serum chemokines in man. European Journal of Medical Research, 10(2), 76-80.