Effects of endotoxin on serum chemokines in man

Svenja Schinkel, C. Schinkel, V. Pollard, R. Garofallo, H. Heberle, P. Reisner, John Papaconstantinou, David Herndon

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Aim: Endotoxin is known to be a primary initiator of sepsis and septic shock. Migration of immunocompetent cells due to chemotactic attraction plays a central role in the initiation of the immune response. Two major groups of chemokines can be distinguished: C-x-C chemokines like Interleukin-8 attract mainly neutrophils, C-C chemokines (e.g. RANTES) attract monocytes and T-cells. The aim of this study was to get further insight into chemokine profiles after a single endotoxin bolus in man. Materials and Methods: We investigated the effect of systemically administered endotoxin (4ng/kg BW i.v.) in 8 healthy volunteers. Clinical data (heart rate, mean arterial pressure, temperature), serum levels of IL-8, and RANTES, as well as white blood cell count were obtained before and hourly for five hours after endotoxin administration. Results: Heart rate and MAP showed significant changes (p<0.05) after 2-3 hours. All volunteers presented with low-grade fever after 2 hours. WBC was elevated 43% and 63% after 4 and 5 hours, respectively. Both chemokines were significantly different from baseline two hours after endotoxin challenge: While IL-8 was significantly increased RANTES serum levels were diminished. Conclusion: From our data we conclude that this endotoxin model was effective to mimic the clinical appearance of sepsis. Chemokines like IL-8 and RANTES are integrated in the early immune response to endotoxin challenge in man.

Original languageEnglish (US)
Pages (from-to)76-80
Number of pages5
JournalEuropean Journal of Medical Research
Volume10
Issue number2
StatePublished - Feb 28 2005

Fingerprint

Chemokines
Endotoxins
Chemokine CCL5
Interleukin-8
Serum
CC Chemokines
Sepsis
Heart Rate
Septic Shock
Leukocyte Count
Cell Movement
Monocytes
Volunteers
Healthy Volunteers
Arterial Pressure
Neutrophils
Fever
T-Lymphocytes
Temperature

Keywords

  • Chemokines
  • Endotoxin
  • IL-8
  • RANTES
  • Sepsis
  • Volunteer study

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Schinkel, S., Schinkel, C., Pollard, V., Garofallo, R., Heberle, H., Reisner, P., ... Herndon, D. (2005). Effects of endotoxin on serum chemokines in man. European Journal of Medical Research, 10(2), 76-80.

Effects of endotoxin on serum chemokines in man. / Schinkel, Svenja; Schinkel, C.; Pollard, V.; Garofallo, R.; Heberle, H.; Reisner, P.; Papaconstantinou, John; Herndon, David.

In: European Journal of Medical Research, Vol. 10, No. 2, 28.02.2005, p. 76-80.

Research output: Contribution to journalArticle

Schinkel, S, Schinkel, C, Pollard, V, Garofallo, R, Heberle, H, Reisner, P, Papaconstantinou, J & Herndon, D 2005, 'Effects of endotoxin on serum chemokines in man', European Journal of Medical Research, vol. 10, no. 2, pp. 76-80.
Schinkel S, Schinkel C, Pollard V, Garofallo R, Heberle H, Reisner P et al. Effects of endotoxin on serum chemokines in man. European Journal of Medical Research. 2005 Feb 28;10(2):76-80.
Schinkel, Svenja ; Schinkel, C. ; Pollard, V. ; Garofallo, R. ; Heberle, H. ; Reisner, P. ; Papaconstantinou, John ; Herndon, David. / Effects of endotoxin on serum chemokines in man. In: European Journal of Medical Research. 2005 ; Vol. 10, No. 2. pp. 76-80.
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