Effects of endotoxin on serum chemokines in man

Svenja Schinkel, C. Schinkel, V. Pollard, R. Garofallo, H. Heberle, P. Reisner, J. Papaconstantinou, David Herndon

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


Aim: Endotoxin is known to be a primary initiator of sepsis and septic shock. Migration of immunocompetent cells due to chemotactic attraction plays a central role in the initiation of the immune response. Two major groups of chemokines can be distinguished: C-x-C chemokines like Interleukin-8 attract mainly neutrophils, C-C chemokines (e.g. RANTES) attract monocytes and T-cells. The aim of this study was to get further insight into chemokine profiles after a single endotoxin bolus in man. Materials and Methods: We investigated the effect of systemically administered endotoxin (4ng/kg BW i.v.) in 8 healthy volunteers. Clinical data (heart rate, mean arterial pressure, temperature), serum levels of IL-8, and RANTES, as well as white blood cell count were obtained before and hourly for five hours after endotoxin administration. Results: Heart rate and MAP showed significant changes (p<0.05) after 2-3 hours. All volunteers presented with low-grade fever after 2 hours. WBC was elevated 43% and 63% after 4 and 5 hours, respectively. Both chemokines were significantly different from baseline two hours after endotoxin challenge: While IL-8 was significantly increased RANTES serum levels were diminished. Conclusion: From our data we conclude that this endotoxin model was effective to mimic the clinical appearance of sepsis. Chemokines like IL-8 and RANTES are integrated in the early immune response to endotoxin challenge in man.

Original languageEnglish (US)
Pages (from-to)76-80
Number of pages5
JournalEuropean Journal of Medical Research
Issue number2
StatePublished - Feb 28 2005


  • Chemokines
  • Endotoxin
  • IL-8
  • Sepsis
  • Volunteer study

ASJC Scopus subject areas

  • General Medicine


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