Effects of excreted/secreted antigens of Toxoplasma gondii on CD4 + CD25 + Foxp3 + T cells and NK cells of melanoma-bearing mice

Yu Meng Jiao, Li Zhang, Yi Yue Ge, Yue Jin Liang, Yong Wang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objective: To explore the effects of excreted/secreted antigens (ESA) of Toxoplasma gondii on CD4 +CD25 + Foxp3 + T cells and NK cells of melanoma-bearing mice, as well as the tumor growth. Methods: B16F10 (denoted B16) tumor cells were cultured in complete medium and maintained by serial passage in vitro. The 2 × 10 5 B16 tumor cells were injected into the right flank of the mouse to establish the tumor-bearing mice model. Mice were randomly divided into four groups, namely PBS, B16F10, PBS/ESA and B16F10/ESA groups after ESA injections. On days 2, 4 and 6 post-ESA injection, the spleens were removed. The percentage of CD4 +CD25 + Foxp3 + T cells and NK cells in splenocytes were determined by flow cytometry; the suppression functions of CD4 +CD25 + Tregs and the NK cell activity were detected by WST-8 and LDH methods, respectively. The tumor growth of each group was measured. Results: On Days 4 and 6 post-ESA injection, the percentages of CD4 +CD25 + Foxp3 + T cells in splenocytes of the B16F10/ESA-injected mice decreased being (1.65±0.18)% and (1.56±0.17)%, respectively, and compared with those in the B16-injected mice [(2.47±0.10)% and (2.82±0.12)%], there were significant differences (both P values +CD25 + Tregs of the B16F10/ESA-injected mice decreased markedly on Day 4 (50.03%) and Day 6 (50%) compared with those in the control (75.03% and 78.14%) post-ESA injection, there were significant difference (both P values 3 was significantly smaller than that of control [(9027.46±1362.01)] mm 3 (P <0.05) when measured at Day 35 post-tumor innoculation. Conclusions: T. gondii ESA can downregulate CD4 +CD25 + Tregs while upregulating NK cells of B16 tumor-bearing mice quantitatively and functionally, therefore plays a role in suppression of tumor growth.

Original languageEnglish (US)
Pages (from-to)301-306
Number of pages6
JournalChinese Journal of Schistosomiasis Control
Volume23
Issue number3
StatePublished - Jun 15 2011
Externally publishedYes

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Toxoplasma
Natural Killer Cells
Melanoma
T-Lymphocytes
Antigens
Neoplasms
Injections
Growth
Cultured Tumor Cells
Serial Passage
Flow Cytometry
Down-Regulation
Spleen

Keywords

  • CD4 CD25 Foxp3 T cell
  • Excreted-secreted antigens (ESA)
  • Melanoma
  • NK cell
  • Toxoplasma gondii

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Effects of excreted/secreted antigens of Toxoplasma gondii on CD4 + CD25 + Foxp3 + T cells and NK cells of melanoma-bearing mice. / Jiao, Yu Meng; Zhang, Li; Ge, Yi Yue; Liang, Yue Jin; Wang, Yong.

In: Chinese Journal of Schistosomiasis Control, Vol. 23, No. 3, 15.06.2011, p. 301-306.

Research output: Contribution to journalArticle

Jiao, Yu Meng ; Zhang, Li ; Ge, Yi Yue ; Liang, Yue Jin ; Wang, Yong. / Effects of excreted/secreted antigens of Toxoplasma gondii on CD4 + CD25 + Foxp3 + T cells and NK cells of melanoma-bearing mice. In: Chinese Journal of Schistosomiasis Control. 2011 ; Vol. 23, No. 3. pp. 301-306.
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abstract = "Objective: To explore the effects of excreted/secreted antigens (ESA) of Toxoplasma gondii on CD4 +CD25 + Foxp3 + T cells and NK cells of melanoma-bearing mice, as well as the tumor growth. Methods: B16F10 (denoted B16) tumor cells were cultured in complete medium and maintained by serial passage in vitro. The 2 × 10 5 B16 tumor cells were injected into the right flank of the mouse to establish the tumor-bearing mice model. Mice were randomly divided into four groups, namely PBS, B16F10, PBS/ESA and B16F10/ESA groups after ESA injections. On days 2, 4 and 6 post-ESA injection, the spleens were removed. The percentage of CD4 +CD25 + Foxp3 + T cells and NK cells in splenocytes were determined by flow cytometry; the suppression functions of CD4 +CD25 + Tregs and the NK cell activity were detected by WST-8 and LDH methods, respectively. The tumor growth of each group was measured. Results: On Days 4 and 6 post-ESA injection, the percentages of CD4 +CD25 + Foxp3 + T cells in splenocytes of the B16F10/ESA-injected mice decreased being (1.65±0.18){\%} and (1.56±0.17){\%}, respectively, and compared with those in the B16-injected mice [(2.47±0.10){\%} and (2.82±0.12){\%}], there were significant differences (both P values +CD25 + Tregs of the B16F10/ESA-injected mice decreased markedly on Day 4 (50.03{\%}) and Day 6 (50{\%}) compared with those in the control (75.03{\%} and 78.14{\%}) post-ESA injection, there were significant difference (both P values 3 was significantly smaller than that of control [(9027.46±1362.01)] mm 3 (P <0.05) when measured at Day 35 post-tumor innoculation. Conclusions: T. gondii ESA can downregulate CD4 +CD25 + Tregs while upregulating NK cells of B16 tumor-bearing mice quantitatively and functionally, therefore plays a role in suppression of tumor growth.",
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AU - Jiao, Yu Meng

AU - Zhang, Li

AU - Ge, Yi Yue

AU - Liang, Yue Jin

AU - Wang, Yong

PY - 2011/6/15

Y1 - 2011/6/15

N2 - Objective: To explore the effects of excreted/secreted antigens (ESA) of Toxoplasma gondii on CD4 +CD25 + Foxp3 + T cells and NK cells of melanoma-bearing mice, as well as the tumor growth. Methods: B16F10 (denoted B16) tumor cells were cultured in complete medium and maintained by serial passage in vitro. The 2 × 10 5 B16 tumor cells were injected into the right flank of the mouse to establish the tumor-bearing mice model. Mice were randomly divided into four groups, namely PBS, B16F10, PBS/ESA and B16F10/ESA groups after ESA injections. On days 2, 4 and 6 post-ESA injection, the spleens were removed. The percentage of CD4 +CD25 + Foxp3 + T cells and NK cells in splenocytes were determined by flow cytometry; the suppression functions of CD4 +CD25 + Tregs and the NK cell activity were detected by WST-8 and LDH methods, respectively. The tumor growth of each group was measured. Results: On Days 4 and 6 post-ESA injection, the percentages of CD4 +CD25 + Foxp3 + T cells in splenocytes of the B16F10/ESA-injected mice decreased being (1.65±0.18)% and (1.56±0.17)%, respectively, and compared with those in the B16-injected mice [(2.47±0.10)% and (2.82±0.12)%], there were significant differences (both P values +CD25 + Tregs of the B16F10/ESA-injected mice decreased markedly on Day 4 (50.03%) and Day 6 (50%) compared with those in the control (75.03% and 78.14%) post-ESA injection, there were significant difference (both P values 3 was significantly smaller than that of control [(9027.46±1362.01)] mm 3 (P <0.05) when measured at Day 35 post-tumor innoculation. Conclusions: T. gondii ESA can downregulate CD4 +CD25 + Tregs while upregulating NK cells of B16 tumor-bearing mice quantitatively and functionally, therefore plays a role in suppression of tumor growth.

AB - Objective: To explore the effects of excreted/secreted antigens (ESA) of Toxoplasma gondii on CD4 +CD25 + Foxp3 + T cells and NK cells of melanoma-bearing mice, as well as the tumor growth. Methods: B16F10 (denoted B16) tumor cells were cultured in complete medium and maintained by serial passage in vitro. The 2 × 10 5 B16 tumor cells were injected into the right flank of the mouse to establish the tumor-bearing mice model. Mice were randomly divided into four groups, namely PBS, B16F10, PBS/ESA and B16F10/ESA groups after ESA injections. On days 2, 4 and 6 post-ESA injection, the spleens were removed. The percentage of CD4 +CD25 + Foxp3 + T cells and NK cells in splenocytes were determined by flow cytometry; the suppression functions of CD4 +CD25 + Tregs and the NK cell activity were detected by WST-8 and LDH methods, respectively. The tumor growth of each group was measured. Results: On Days 4 and 6 post-ESA injection, the percentages of CD4 +CD25 + Foxp3 + T cells in splenocytes of the B16F10/ESA-injected mice decreased being (1.65±0.18)% and (1.56±0.17)%, respectively, and compared with those in the B16-injected mice [(2.47±0.10)% and (2.82±0.12)%], there were significant differences (both P values +CD25 + Tregs of the B16F10/ESA-injected mice decreased markedly on Day 4 (50.03%) and Day 6 (50%) compared with those in the control (75.03% and 78.14%) post-ESA injection, there were significant difference (both P values 3 was significantly smaller than that of control [(9027.46±1362.01)] mm 3 (P <0.05) when measured at Day 35 post-tumor innoculation. Conclusions: T. gondii ESA can downregulate CD4 +CD25 + Tregs while upregulating NK cells of B16 tumor-bearing mice quantitatively and functionally, therefore plays a role in suppression of tumor growth.

KW - CD4 CD25 Foxp3 T cell

KW - Excreted-secreted antigens (ESA)

KW - Melanoma

KW - NK cell

KW - Toxoplasma gondii

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