Effects of gamma-irradiation on the electrokinetic properties of purple membranes

Virjinia Doltchinkova, Svetla Stoilova, Maria Baldjiiska

Research output: Contribution to journalArticle

Abstract

The effect of gamma-irradiation (5, 10 and 15 Gy) on the kinetic surface charge of purple membranes (PM) was followed by means of particle microelectrophoresis. The changes in electrophoretic mobility (EPM) were examined at 2, 5 and 26 h, respectively, following irradiation of native PM, and at 2, 26, 50 h and 5 days following irradiation of delipidated PM. It was concluded that the high inhomogeneity of the suspension, even after sonication, largely affects the measured zeta-potential. The 15-Gy treatment significantly increased the net negative surface charge density at 5 and 26 h after irradiation of native PM. However, the opposite effect of approximately twofold reduction of EPM values was derived from simultaneous studies concerning their delipidated form. Low irradiation doses clearly induced an enhancement of negative surface charge density at 2 h post-exposure as well as the formation of unstable structures of delipidated PM. The changes in electrokinetic properties might reflect the specific aggregate formation in both native and delipidated PM. It was suggested that the effect observed of both types of PM was mainly a structural phenomenon possibly related to the modification of functionally active residues.

Original languageEnglish (US)
Pages (from-to)41-45
Number of pages5
JournalRadiation and Environmental Biophysics
Volume37
Issue number1
DOIs
StatePublished - Apr 1998
Externally publishedYes

Fingerprint

Purple Membrane
electrokinetics
gamma radiation
irradiation
Irradiation
membrane
membranes
Membranes
Surface charge
Electrophoretic mobility
Charge density
Sonication
kinetics
Zeta potential
effect
inhomogeneity
Dosimetry
dosage
Suspensions
Kinetics

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Environmental Science(all)
  • Environmental Chemistry
  • Radiology Nuclear Medicine and imaging
  • Biophysics
  • Radiation

Cite this

Effects of gamma-irradiation on the electrokinetic properties of purple membranes. / Doltchinkova, Virjinia; Stoilova, Svetla; Baldjiiska, Maria.

In: Radiation and Environmental Biophysics, Vol. 37, No. 1, 04.1998, p. 41-45.

Research output: Contribution to journalArticle

Doltchinkova, Virjinia ; Stoilova, Svetla ; Baldjiiska, Maria. / Effects of gamma-irradiation on the electrokinetic properties of purple membranes. In: Radiation and Environmental Biophysics. 1998 ; Vol. 37, No. 1. pp. 41-45.
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