Effects of gastrin on 3′,5′-cyclic adenosine monophosphate, intracellular calcium, and phosphatidylinositol hydrolysis in human colon cancer cells

Jin Ishizuka, Courtney Townsend, Richard J. Bold, Jean Martinez, Marc Rodriguez, James C. Thompson

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Gastrin is a trophic factor for some human colon cancer cells. However, the signal-transduction pathways by which gastrin regulates growth are still unknown. We examined the effect of synthetic human gastrin-17 (G-17) on signal-transduction pathways and cell growth using 4 different human colon cancer cell lines (LoVo, COLO 320, HT-29, and HCT116). G-17 stimulated the production of cyclic AMP in LoVo, COLO 320, and HCT116 cells, while G-17 stimulated phosphatidylinositol hydrolysis and mobilization of intracellular calcium in HT-29 cells. The growth-regulatory effect of G-17 on these colon cancer cells (stimulatory on LoVo, COLO 320, and HT-29 cells; inhibitory on HCT116 cells) was well correlated with the effect of G-17 on the signal-transduction pathway in each cell line. We further examined the effect of a selective cholecystokinin-B type receptor antagonist, JMV 320, on G-17-induced signal-transduction pathways and G-17-regulated growth. In each cell line, the effect of JMV 320 on G-17-induced signal-transduction pathways was well correlated with that on G-17-regulated growth. G-17 appears to regulate, at least to some extent, growth of human colon cancer cells through gastrin receptor-linked signal-transduction pathways that are cell-specific.

Original languageEnglish (US)
Pages (from-to)2129-2135
Number of pages7
JournalCancer Research
Volume54
Issue number8
StatePublished - Apr 15 1994

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Gastrins
Phosphatidylinositols
Cyclic AMP
Colonic Neoplasms
Hydrolysis
Calcium
Signal Transduction
Growth
Cholecystokinin B Receptor
HCT116 Cells
HT29 Cells
Cell Line
gastrin 17

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Effects of gastrin on 3′,5′-cyclic adenosine monophosphate, intracellular calcium, and phosphatidylinositol hydrolysis in human colon cancer cells. / Ishizuka, Jin; Townsend, Courtney; Bold, Richard J.; Martinez, Jean; Rodriguez, Marc; Thompson, James C.

In: Cancer Research, Vol. 54, No. 8, 15.04.1994, p. 2129-2135.

Research output: Contribution to journalArticle

Ishizuka, Jin ; Townsend, Courtney ; Bold, Richard J. ; Martinez, Jean ; Rodriguez, Marc ; Thompson, James C. / Effects of gastrin on 3′,5′-cyclic adenosine monophosphate, intracellular calcium, and phosphatidylinositol hydrolysis in human colon cancer cells. In: Cancer Research. 1994 ; Vol. 54, No. 8. pp. 2129-2135.
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