Effects of glutamine-enriched parenteral nutrition on the exocrine pancreas

W. S. Helton, D. O. Jacobs, S. Bonner-Weir, R. Bueno, R. J. Smith, D. W. Wilmore

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Total parenteral nutrition (TPN) is associated with intestinal and pancreatic atrophy and pancreatic exocrine insufficiency. Recent investigations have demonstrated that the addition of glutamine to intravenous feedings attenuates TPN-associated intestinal atrophy. However, the effect of glutamine-supplemented intravenous feedings on the pancreas of intact animals is unknown. This study compared the effects of an intravenous infusion of a 2% glutamine-enriched diet (GLN) with an isonitrogenous, isocaloric diet without glutamine (CONT) on the composition and structure of the exocrine pancreas in laboratory rats with and without a 60% small bowel resection. In nonresected, TPN-fed animals, pancreatic weight was significantly increased in the GLN group when compared to CONT (645 ± 33 g vs 554 ± 20 g, p < 0.05). Nonresected GLN animals also had increased pancreatic DNA (3.82 ± 0.19 mg vs 2.91 ± 0.49 mg, p < 0.005) and protein contents (93.0 ± 5.9 mg vs 76.6 ± 7.0 mg, p = 0.08) compared to control. Similar significant increases in pancreatic weight, DNA, and protein were observed in intestinally resected animals fed the glutamine diet. When data from CONT and GLN animals were pooled and analyzed together, glutamine significantly increased total pancreatic trypsinogen and lipase contents (p < 0.05). The increase in trysinogen in resected GLN animals was significantly greater than in CONT animals (283 ± 22 vs 139 ± 23, p < 0.005). Biochemical and morphometric observations demonstrated that the trophic effects of glutamine on the exocrine pancreas were manifest by acinar hyperplasia and not hypertrophy. Glutamine appears to be an important nutrient for pancreatic exocrine tissue during TPN. The trophic effect of glutamine on the pancreas may have important clinical relevance in reversing or attenuating pancreatic atrophy and insufficiency during intravenous feeding.

Original languageEnglish (US)
Pages (from-to)344-352
Number of pages9
JournalJournal of Parenteral and Enteral Nutrition
Volume14
Issue number4
StatePublished - 1990
Externally publishedYes

Fingerprint

Exocrine Pancreas
Parenteral Nutrition
parenteral feeding
Glutamine
pancreas
glutamine
Total Parenteral Nutrition
total parenteral nutrition
atrophy
Atrophy
Exocrine Pancreatic Insufficiency
animals
Diet
Pancreas
trypsinogen
diet
Trypsinogen
Weights and Measures
DNA
resection

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Helton, W. S., Jacobs, D. O., Bonner-Weir, S., Bueno, R., Smith, R. J., & Wilmore, D. W. (1990). Effects of glutamine-enriched parenteral nutrition on the exocrine pancreas. Journal of Parenteral and Enteral Nutrition, 14(4), 344-352.

Effects of glutamine-enriched parenteral nutrition on the exocrine pancreas. / Helton, W. S.; Jacobs, D. O.; Bonner-Weir, S.; Bueno, R.; Smith, R. J.; Wilmore, D. W.

In: Journal of Parenteral and Enteral Nutrition, Vol. 14, No. 4, 1990, p. 344-352.

Research output: Contribution to journalArticle

Helton, WS, Jacobs, DO, Bonner-Weir, S, Bueno, R, Smith, RJ & Wilmore, DW 1990, 'Effects of glutamine-enriched parenteral nutrition on the exocrine pancreas', Journal of Parenteral and Enteral Nutrition, vol. 14, no. 4, pp. 344-352.
Helton WS, Jacobs DO, Bonner-Weir S, Bueno R, Smith RJ, Wilmore DW. Effects of glutamine-enriched parenteral nutrition on the exocrine pancreas. Journal of Parenteral and Enteral Nutrition. 1990;14(4):344-352.
Helton, W. S. ; Jacobs, D. O. ; Bonner-Weir, S. ; Bueno, R. ; Smith, R. J. ; Wilmore, D. W. / Effects of glutamine-enriched parenteral nutrition on the exocrine pancreas. In: Journal of Parenteral and Enteral Nutrition. 1990 ; Vol. 14, No. 4. pp. 344-352.
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