Effects of hemoglobin-based blood substitutes on vasoactivity of rat aortic rings

Luiz Francisco Poli De Figueiredo, Sharon H. Nelson, Mali Mathru, Mauricio Rocha E Silva, George Kramer

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Our objective is to characterize the vasoactive properties of a 10% αα diaspirin cross-linked human hemoglobin (ααHb) and to test the hypothesis that sodium nitroprusside (SNP)-induced relaxation is inhibited in the presence of ααHb. Experiments were performed on aortic rings from 18 Wistar rats; the rings were suspended in aerated Krebs solution. Changes in isometric tension were measured to increasing concentrations of ααHb (1.8 × 10-9 to 10-4 M) on phenylephrine (PE)-induced contraction (3 × 10-7 M), on acetylcholine (ACh)-induced relaxation (10-8 to 10-6 M), on SNP-induced relaxation (10-9 and 10-8 M), and on PE-induced contraction with an endothelin-1 (ET1) receptor antagonist, BQ123 (10-5 M). Control rings received no ααHb. A concentration-dependent increase of the PE-precontraction (1.3%, 6.8%, 17.4%, and 34%, respectively) as well as the inhibition and reversal of ACh-induced relaxation was observed after ααHb. The presence of ααHb decreased the SNP-induced relaxation in the presence or absence of endothelium. The relaxation induced by SNP was reduced with time in the presence, but not in the absence, of ααHb. In conclusion, although pharmacological modulation of the vasoconstriction is possible with nitric oxide donors, our findings suggest that in the clinical setting, large sustained donor doses may be required.

Original languageEnglish
Pages (from-to)928-933
Number of pages6
JournalArtificial Organs
Volume25
Issue number11
DOIs
StatePublished - 2001

Fingerprint

Blood Substitutes
Hemoglobin
Rats
Nitroprusside
Hemoglobins
Phenylephrine
Sodium
Acetylcholine
Hemoglobin A
Endothelin A Receptors
Nitric Oxide Donors
Vasoconstriction
Endothelium
Wistar Rats
Nitric oxide
Hemoglobin based blood substitutes
Pharmacology
Modulation

Keywords

  • Blood substitutes
  • Hemoglobin
  • Isolated vessels
  • Nitric oxide donors
  • Vasoconstriction

ASJC Scopus subject areas

  • Biophysics

Cite this

Effects of hemoglobin-based blood substitutes on vasoactivity of rat aortic rings. / De Figueiredo, Luiz Francisco Poli; Nelson, Sharon H.; Mathru, Mali; Rocha E Silva, Mauricio; Kramer, George.

In: Artificial Organs, Vol. 25, No. 11, 2001, p. 928-933.

Research output: Contribution to journalArticle

De Figueiredo, Luiz Francisco Poli ; Nelson, Sharon H. ; Mathru, Mali ; Rocha E Silva, Mauricio ; Kramer, George. / Effects of hemoglobin-based blood substitutes on vasoactivity of rat aortic rings. In: Artificial Organs. 2001 ; Vol. 25, No. 11. pp. 928-933.
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