Effects of in ovo injection of stimulant solutions on growth and yolk utilization in broiler embryos

B. M. McGruder, W. Zhai, M. M. Keralapurath, P. D. Gerard, E. D. Peebles

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Effects of the automated in ovo injection of stimulant solutions (200 μL) on growth and yolk utilization in the broiler embryo was investigated in 3 trials. Treatment solutions were injected into the amnion of embryos on d 16 of incubation. Treatment effects on 0 to 18 d percentage incubational egg weight loss, proportional embryo BW, proportional yolk sac weight and embryo and yolk sac moisture contents on d 18 were determined. In trial 1, 1 mM caffeine decreased proportional embryo BW and increased percentage embryo mortality and moisture content. At 1 × 10 -3 and 10 -6 mM concentrations, caffeine decreased proportional embryo BW and at a 1 × 10 -6 mM concentration, caffeine increased percentage embryo moisture concentration. However, caffeine at concentrations of 1 × 10 -3, 10 -6 and 10 -9 mM had no detrimental effect on embryo survivability. Theophylline, creatine, or phosphocreatine at a 1 mM concentration in trial 2 and theophylline, creatine, or L-arginine at a 1 mM concentration in combination with 5.5 mM potassium chloride in trial 3 did not affect any of the parameters examined. The current study indicated that except for 1 mM caffeine, the tested stimulant solutions had no detrimental effects on embryogenesis, suggesting their potential use as stimulants in the commercial injection of broiler hatching eggs. However, they may have to be used at different concentrations or at different times of incubation than those used in this study in order to improve embryo growth and yolk nutrient utilization, while having no detrimental effect on embryo survivability.

Original languageEnglish (US)
Pages (from-to)338-343
Number of pages6
JournalInternational Journal of Poultry Science
Volume10
Issue number5
DOIs
StatePublished - 2011
Externally publishedYes

Fingerprint

embryo (animal)
Embryonic Structures
broiler chickens
injection
Injections
caffeine
Growth
Caffeine
theophylline
creatine
yolk sac
Yolk Sac
Creatine
Theophylline
phosphocreatine
amnion
water content
embryo sac
potassium chloride
embryonic mortality

Keywords

  • Broiler
  • Embryo
  • In ovo injection
  • Stimulant
  • Yolk

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology

Cite this

Effects of in ovo injection of stimulant solutions on growth and yolk utilization in broiler embryos. / McGruder, B. M.; Zhai, W.; Keralapurath, M. M.; Gerard, P. D.; Peebles, E. D.

In: International Journal of Poultry Science, Vol. 10, No. 5, 2011, p. 338-343.

Research output: Contribution to journalArticle

McGruder, B. M. ; Zhai, W. ; Keralapurath, M. M. ; Gerard, P. D. ; Peebles, E. D. / Effects of in ovo injection of stimulant solutions on growth and yolk utilization in broiler embryos. In: International Journal of Poultry Science. 2011 ; Vol. 10, No. 5. pp. 338-343.
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