TY - JOUR
T1 - Effects of incremental starvation on gut mucosa
AU - Chappell, Vicky L.
AU - Thompson, Mark D.
AU - Jeschke, Marc G.
AU - Chung, Dai H.
AU - Thompson, James C.
AU - Wolf, Steven E.
N1 - Funding Information:
Manuscript received April 23, 2002; accepted December 26, 2002. From the *Department of Surgery, The University of Texas Medical Branch; and yShriners Hospitals for Children, Galveston, Texas, USA. Funding support was provided by the National Institutes of Health (RO1 DK15241) and Shriners Hospitals for Children grant (00GAL009). Address for reprint requests: Dr. Steven E. Wolf, Department of Surgery Shriners Hospitals for Children 815 Market Street Galveston, Texas 77550, USA.
PY - 2003/4/1
Y1 - 2003/4/1
N2 - Starvation induces gut mucosal atrophy, but the effects of progressive dietary restriction are not defined. The study's purpose was to determine the effects of incremental starvation on gut epithelial cell turnover. After food intake of mice was determined, they were divided into five groups: control (ad libitum fed), 75% normal intake, 50% intake, 25% intake, and fasted. Mice were killed after 48 hours, and the proximal small bowel were assessed for weight and protein content. Histologic specimens were examined for villus morphology, apoptosis, and proliferation. After 48 hr of diet restriction, bowel weight decreased in the 50% intake, 25% intake, and fasted groups. Villus density also decreased in the fasted group. Proliferation progressively decreased in the diet-restricted groups. Apoptosis increased in the fasted group, primarily in the villus tip. In conclusion, incremental starvation produces progressive small bowel atrophy. The mechanism involves both decreased gut epithelial cell proliferation and increased apoptosis.
AB - Starvation induces gut mucosal atrophy, but the effects of progressive dietary restriction are not defined. The study's purpose was to determine the effects of incremental starvation on gut epithelial cell turnover. After food intake of mice was determined, they were divided into five groups: control (ad libitum fed), 75% normal intake, 50% intake, 25% intake, and fasted. Mice were killed after 48 hours, and the proximal small bowel were assessed for weight and protein content. Histologic specimens were examined for villus morphology, apoptosis, and proliferation. After 48 hr of diet restriction, bowel weight decreased in the 50% intake, 25% intake, and fasted groups. Villus density also decreased in the fasted group. Proliferation progressively decreased in the diet-restricted groups. Apoptosis increased in the fasted group, primarily in the villus tip. In conclusion, incremental starvation produces progressive small bowel atrophy. The mechanism involves both decreased gut epithelial cell proliferation and increased apoptosis.
KW - Diet restriction
KW - Gut apoptosis
KW - Gut proliferation
KW - Starvation
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U2 - 10.1023/A:1022849112100
DO - 10.1023/A:1022849112100
M3 - Article
C2 - 12741469
AN - SCOPUS:0345505264
SN - 0163-2116
VL - 48
SP - 765
EP - 769
JO - Digestive Diseases and Sciences
JF - Digestive Diseases and Sciences
IS - 4
ER -