Effects of intravenous sulfide during porcine aortic occlusion-induced kidney ischemia/reperfusion injury

Florian Simon, Angelika Scheuerle, Michael Gröger, Bettina Stahl, Ulrich Wachter, Josef Vogt, Günter Speit, Balázs Hauser, Peter Möller, Enrico Calzia, Csaba Szabó, Hubert Schelzig, Michael Georgieff, Peter Radermacher, Florian Wagner

Research output: Contribution to journalArticlepeer-review

55 Scopus citations


In rodents, inhaled H 2S and injection of H 2S donors protected against kidney ischemia/reperfusion (I/R) injury. During porcine aortic occlusion, the H 2S donor Na2S (sulfide) reduced energy expenditure and decreased the noradrenaline requirements needed to maintain hemodynamic targets during early reperfusion. Therefore, we tested the hypothesis whether sulfide pretreatment may also ameliorate organ function in porcine aortic occlusion-induced kidney I/R injury. Anesthetized, ventilated, and instrumented pigs randomly received either sulfide or vehicle and underwent 90 min of kidney ischemia using intraaortic balloon-occlusion, and 8 h of reperfusion. During reperfusion, noradrenaline was titrated to maintain blood pressure at baseline levels. Sulfide attenuated the fall in creatinine clearance and the rise in creatinine blood levels, whereas renal blood flow and fractional Na excretion were comparable. Sulfide also lowered the blood IL-6, IL-1β, and nitrite + nitrate concentrations, which coincided with reduced kidney oxidative DNA base damage and iNOS expression, and attenuated glomerular histological injury as assessed by the incidence of glomerular tubularization. While expression of heme oxygenase 1 and cleaved caspase 3 did not differ, sulfide reduced the expression Bcl-xL and increased the activation of nuclear transcription factor κB. During porcine aortic occlusion-induced kidney I/R injury, sulfide pretreatment attenuated tissue injury and organ dysfunction as a result of reduced inflammation and oxidative and nitrosative stress. The higher nuclear transcription factor κB activation was probably due to the drop in temperature.

Original languageEnglish (US)
Pages (from-to)156-163
Number of pages8
Issue number2
StatePublished - Feb 2011


  • Bcl-xL
  • Glomerular filtration
  • TUNEL staining
  • caspase 3
  • comet assay
  • glomerular tubularization
  • heme oxygenase 1
  • iNOS
  • isoprostane
  • nuclear transcription factor κB
  • tubular reabsorption

ASJC Scopus subject areas

  • Emergency Medicine
  • Critical Care and Intensive Care Medicine


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