Effects of low oxygen levels on the expression and function of transporter OCTN2 in BeWo cells

Erik Rytting, Kenneth L. Audus

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Although hypoxia is normal in early pregnancy, low placental oxygen concentrations later in pregnancy are often linked to complications such as pre-eclampsia and intrauterine growth restriction. The effects of low oxygen levels on drug and nutrient uptake via the organic cation transporter OCTN2 has been studied in BeWo cells, an in-vitro model of human trophoblast. BeWo cells were cultured under 20% (control) or 2% O2 (hypoxia) for 48 h before each experiment. In-vitro hypoxia was also simulated by the addition of CoCl2 to the cell culture medium. RT-PCR indicated increased transcription of OCTN2 in BeWo cells cultured under hypoxia, but Western blots did not show a corresponding increase in the amount of OCTN2 protein in the hypoxic cells compared with control. Hypoxia resulted in significant reductions in OCTN2-mediated carnitine uptake. Decreased placental transport of carnitine may lead to symptoms of carnitine deficiency in infants from hypoxic pregnancies, whether caused by high altitude, pre-eclampsia or other factors. The OCTN1 substrate ergothioneine reversed the effects of hypoxia on carnitine transport, but identical concentrations of N-acetylcysteine, another water-soluble intracellular antioxidant, did not have the same effect.

Original languageEnglish (US)
Pages (from-to)1095-1102
Number of pages8
JournalJournal of Pharmacy and Pharmacology
Volume59
Issue number8
DOIs
StatePublished - Aug 2007
Externally publishedYes

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Carnitine
Oxygen
Pre-Eclampsia
Pregnancy
Cultured Cells
Ergothioneine
Trophoblasts
Acetylcysteine
Culture Media
Hypoxia
Cell Culture Techniques
Antioxidants
Western Blotting
Polymerase Chain Reaction
Water
Growth
Pharmaceutical Preparations
Proteins
In Vitro Techniques

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

Cite this

Effects of low oxygen levels on the expression and function of transporter OCTN2 in BeWo cells. / Rytting, Erik; Audus, Kenneth L.

In: Journal of Pharmacy and Pharmacology, Vol. 59, No. 8, 08.2007, p. 1095-1102.

Research output: Contribution to journalArticle

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