Effects of nerve growth factor on catalase and glutathione peroxidase in a hydrogen peroxide-resistant pheochromocytoma subclone

George R. Jackson, Deepa Sampath, Karin Werrbach-Perez, J. Regino Perez-Polo

    Research output: Contribution to journalArticle

    42 Citations (Scopus)

    Abstract

    Stepwise selection in increasing H2O2 concentrations was used to obtain a PC12 cell variant designated HPR. This variant was stably resistant to H2O2 as compared with the parental PC12 cell line. HPR cells responded to nerve factor (NGF) by further enhancing H2O2 resistance. This variant was subcloned by limiting dilution to obtain the line referred to as HPR-C, which was stably resistant to H2O2 toxicity and retained NGF responses, including morphologic changes and further reduction of H2O2 toxicity. When compared with the parental PC12 line, the HPR-C subclone did not have higher levels of catalase or glutathione peroxidase (GSH Px) activity or mRNA expression (as assessed by PCR analysis of cDNA reverse transcribed from total cellular RNA). HPR-C cells retained the ability to respond to NGF treatment by increasing catalase and GSH Px activity and expression. These data suggest that the protective effects of conditioning lesions, unlike those of neurotrophins, are in part independent of changes in the activity or expression of antioxidant enzymes.

    Original languageEnglish (US)
    Pages (from-to)69-76
    Number of pages8
    JournalBrain Research
    Volume634
    Issue number1
    DOIs
    StatePublished - Jan 14 1994

    Fingerprint

    Pheochromocytoma
    Nerve Growth Factor
    Glutathione Peroxidase
    Catalase
    Hydrogen Peroxide
    PC12 Cells
    Nerve Growth Factors
    Complementary DNA
    Antioxidants
    RNA
    Cell Line
    Polymerase Chain Reaction
    Messenger RNA
    Enzymes

    Keywords

    • Catalase
    • Cell death
    • Glutathione peroxidase
    • HO
    • NGF
    • Oxidant resistance
    • PC12

    ASJC Scopus subject areas

    • Developmental Biology
    • Molecular Biology
    • Clinical Neurology
    • Neuroscience(all)

    Cite this

    Effects of nerve growth factor on catalase and glutathione peroxidase in a hydrogen peroxide-resistant pheochromocytoma subclone. / Jackson, George R.; Sampath, Deepa; Werrbach-Perez, Karin; Perez-Polo, J. Regino.

    In: Brain Research, Vol. 634, No. 1, 14.01.1994, p. 69-76.

    Research output: Contribution to journalArticle

    Jackson, George R. ; Sampath, Deepa ; Werrbach-Perez, Karin ; Perez-Polo, J. Regino. / Effects of nerve growth factor on catalase and glutathione peroxidase in a hydrogen peroxide-resistant pheochromocytoma subclone. In: Brain Research. 1994 ; Vol. 634, No. 1. pp. 69-76.
    @article{1023d4725ab54d408ac7b92b98a84a44,
    title = "Effects of nerve growth factor on catalase and glutathione peroxidase in a hydrogen peroxide-resistant pheochromocytoma subclone",
    abstract = "Stepwise selection in increasing H2O2 concentrations was used to obtain a PC12 cell variant designated HPR. This variant was stably resistant to H2O2 as compared with the parental PC12 cell line. HPR cells responded to nerve factor (NGF) by further enhancing H2O2 resistance. This variant was subcloned by limiting dilution to obtain the line referred to as HPR-C, which was stably resistant to H2O2 toxicity and retained NGF responses, including morphologic changes and further reduction of H2O2 toxicity. When compared with the parental PC12 line, the HPR-C subclone did not have higher levels of catalase or glutathione peroxidase (GSH Px) activity or mRNA expression (as assessed by PCR analysis of cDNA reverse transcribed from total cellular RNA). HPR-C cells retained the ability to respond to NGF treatment by increasing catalase and GSH Px activity and expression. These data suggest that the protective effects of conditioning lesions, unlike those of neurotrophins, are in part independent of changes in the activity or expression of antioxidant enzymes.",
    keywords = "Catalase, Cell death, Glutathione peroxidase, HO, NGF, Oxidant resistance, PC12",
    author = "Jackson, {George R.} and Deepa Sampath and Karin Werrbach-Perez and Perez-Polo, {J. Regino}",
    year = "1994",
    month = "1",
    day = "14",
    doi = "10.1016/0006-8993(94)90259-3",
    language = "English (US)",
    volume = "634",
    pages = "69--76",
    journal = "Brain Research",
    issn = "0006-8993",
    publisher = "Elsevier",
    number = "1",

    }

    TY - JOUR

    T1 - Effects of nerve growth factor on catalase and glutathione peroxidase in a hydrogen peroxide-resistant pheochromocytoma subclone

    AU - Jackson, George R.

    AU - Sampath, Deepa

    AU - Werrbach-Perez, Karin

    AU - Perez-Polo, J. Regino

    PY - 1994/1/14

    Y1 - 1994/1/14

    N2 - Stepwise selection in increasing H2O2 concentrations was used to obtain a PC12 cell variant designated HPR. This variant was stably resistant to H2O2 as compared with the parental PC12 cell line. HPR cells responded to nerve factor (NGF) by further enhancing H2O2 resistance. This variant was subcloned by limiting dilution to obtain the line referred to as HPR-C, which was stably resistant to H2O2 toxicity and retained NGF responses, including morphologic changes and further reduction of H2O2 toxicity. When compared with the parental PC12 line, the HPR-C subclone did not have higher levels of catalase or glutathione peroxidase (GSH Px) activity or mRNA expression (as assessed by PCR analysis of cDNA reverse transcribed from total cellular RNA). HPR-C cells retained the ability to respond to NGF treatment by increasing catalase and GSH Px activity and expression. These data suggest that the protective effects of conditioning lesions, unlike those of neurotrophins, are in part independent of changes in the activity or expression of antioxidant enzymes.

    AB - Stepwise selection in increasing H2O2 concentrations was used to obtain a PC12 cell variant designated HPR. This variant was stably resistant to H2O2 as compared with the parental PC12 cell line. HPR cells responded to nerve factor (NGF) by further enhancing H2O2 resistance. This variant was subcloned by limiting dilution to obtain the line referred to as HPR-C, which was stably resistant to H2O2 toxicity and retained NGF responses, including morphologic changes and further reduction of H2O2 toxicity. When compared with the parental PC12 line, the HPR-C subclone did not have higher levels of catalase or glutathione peroxidase (GSH Px) activity or mRNA expression (as assessed by PCR analysis of cDNA reverse transcribed from total cellular RNA). HPR-C cells retained the ability to respond to NGF treatment by increasing catalase and GSH Px activity and expression. These data suggest that the protective effects of conditioning lesions, unlike those of neurotrophins, are in part independent of changes in the activity or expression of antioxidant enzymes.

    KW - Catalase

    KW - Cell death

    KW - Glutathione peroxidase

    KW - HO

    KW - NGF

    KW - Oxidant resistance

    KW - PC12

    UR - http://www.scopus.com/inward/record.url?scp=0028096241&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0028096241&partnerID=8YFLogxK

    U2 - 10.1016/0006-8993(94)90259-3

    DO - 10.1016/0006-8993(94)90259-3

    M3 - Article

    C2 - 8156393

    AN - SCOPUS:0028096241

    VL - 634

    SP - 69

    EP - 76

    JO - Brain Research

    JF - Brain Research

    SN - 0006-8993

    IS - 1

    ER -