Effects of nerve growth factor on catalase and glutathione peroxidase in a hydrogen peroxide-resistant pheochromocytoma subclone

George R. Jackson, Deepa Sampath, Karin Werrbach-Perez, J. Regino Perez-Polo

    Research output: Contribution to journalArticle

    42 Scopus citations

    Abstract

    Stepwise selection in increasing H2O2 concentrations was used to obtain a PC12 cell variant designated HPR. This variant was stably resistant to H2O2 as compared with the parental PC12 cell line. HPR cells responded to nerve factor (NGF) by further enhancing H2O2 resistance. This variant was subcloned by limiting dilution to obtain the line referred to as HPR-C, which was stably resistant to H2O2 toxicity and retained NGF responses, including morphologic changes and further reduction of H2O2 toxicity. When compared with the parental PC12 line, the HPR-C subclone did not have higher levels of catalase or glutathione peroxidase (GSH Px) activity or mRNA expression (as assessed by PCR analysis of cDNA reverse transcribed from total cellular RNA). HPR-C cells retained the ability to respond to NGF treatment by increasing catalase and GSH Px activity and expression. These data suggest that the protective effects of conditioning lesions, unlike those of neurotrophins, are in part independent of changes in the activity or expression of antioxidant enzymes.

    Original languageEnglish (US)
    Pages (from-to)69-76
    Number of pages8
    JournalBrain Research
    Volume634
    Issue number1
    DOIs
    StatePublished - Jan 14 1994

      Fingerprint

    Keywords

    • Catalase
    • Cell death
    • Glutathione peroxidase
    • HO
    • NGF
    • Oxidant resistance
    • PC12

    ASJC Scopus subject areas

    • Neuroscience(all)
    • Molecular Biology
    • Clinical Neurology
    • Developmental Biology

    Cite this