Effects of Nerve Growth Factor on Glutathione Peroxidase and Catalase in PC 12 Cells

Deepa Sampath, George R. Jackson, Karin Werrbach‐Perez, J. Regino Perez‐Polo

    Research output: Contribution to journalArticle

    122 Scopus citations

    Abstract

    Abstract: Nerve growth factor (NGF) is a member of the neuro‐ trophin family and is required for the survival and maintenance of peripheral sympathetic and sensory ganglia. In the CNS, NGF regulates cholinergic expression by basal forebrain cholinergic neurons. NGF also stimulates cellular resistance to oxidative stress in the PC12 cell line and protects PC12 cells from the toxic effects of reactive oxygen species. The hypothesis that NGF protection involves changes in antioxidant enzyme expression was tested by measuring its effects on catalase and glutathione per‐ oxidase (GSH Px) mRNA expression in PC12 cells. NGF increased catalase and GSH Px mRNA levels in PC 12 cells in a time‐ and dose‐dependent manner. There was also a corresponding increase in the enzyme activities of catalase and GSH Px. Thus, NGF can provide cytoprotection to PC12 cells by inducing the free radical scavenging enzymes catalase and GSH Px.

    Original languageEnglish (US)
    Pages (from-to)2476-2479
    Number of pages4
    JournalJournal of neurochemistry
    Volume62
    Issue number6
    DOIs
    StatePublished - Jun 1994

    Keywords

    • Catalase
    • Free radicals
    • Glutathione peroxi‐ dase
    • Nerve growth factor
    • Neurotrophins

    ASJC Scopus subject areas

    • Biochemistry
    • Cellular and Molecular Neuroscience

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