Effects of nerve growth factor on glutathione peroxidase and catalase in PC12 cells

Deepa Sampath, George R. Jackson, Karin Werrbach-Perez, J. Regino Perez-Polo

    Research output: Contribution to journalArticle

    120 Citations (Scopus)

    Abstract

    Nerve growth factor (NGF) is a member of the neurotrophin family and is required for the survival and maintenance of peripheral sympathetic and sensory ganglia. In the CNS, NGF regulates cholinergic expression by basal forebrain cholinergic neurons. NGF also stimulates cellular resistance to oxidative stress in the PC12 cell line and protects PC12 cells from the toxic effects of reactive oxygen species. The hypothesis that NGF protection involves changes in antioxidant enzyme expression was tested by measuring its effects on catalase and glutathione peroxidase (GSH Px) mRNA expression in PC12 cells. NGF increased catalase and GSH Px mRNA levels in PC12 cells in a time- and dose-dependent manner. There was also a corresponding increase in the enzyme activities of catalase and GSH Px. Thus, NGF can provide cytoprotection to PC12 cells by inducing the free radical scavenging enzymes catalase and GSH Px.

    Original languageEnglish (US)
    Pages (from-to)2476-2479
    Number of pages4
    JournalJournal of Neurochemistry
    Volume62
    Issue number6
    StatePublished - Jun 1994

    Fingerprint

    PC12 Cells
    Nerve Growth Factor
    Glutathione Peroxidase
    Catalase
    Cholinergic Agents
    Enzymes
    Sensory Ganglia
    Messenger RNA
    Sympathetic Ganglia
    Cholinergic Neurons
    Oxidative stress
    Cytoprotection
    Poisons
    Scavenging
    Nerve Growth Factors
    Enzyme activity
    Neurons
    Free Radicals
    Reactive Oxygen Species
    Oxidative Stress

    Keywords

    • Catalase
    • Free radicals
    • Glutathione peroxidase
    • Nerve growth factor
    • Neurotrophins

    ASJC Scopus subject areas

    • Biochemistry
    • Cellular and Molecular Neuroscience

    Cite this

    Sampath, D., Jackson, G. R., Werrbach-Perez, K., & Perez-Polo, J. R. (1994). Effects of nerve growth factor on glutathione peroxidase and catalase in PC12 cells. Journal of Neurochemistry, 62(6), 2476-2479.

    Effects of nerve growth factor on glutathione peroxidase and catalase in PC12 cells. / Sampath, Deepa; Jackson, George R.; Werrbach-Perez, Karin; Perez-Polo, J. Regino.

    In: Journal of Neurochemistry, Vol. 62, No. 6, 06.1994, p. 2476-2479.

    Research output: Contribution to journalArticle

    Sampath, D, Jackson, GR, Werrbach-Perez, K & Perez-Polo, JR 1994, 'Effects of nerve growth factor on glutathione peroxidase and catalase in PC12 cells', Journal of Neurochemistry, vol. 62, no. 6, pp. 2476-2479.
    Sampath D, Jackson GR, Werrbach-Perez K, Perez-Polo JR. Effects of nerve growth factor on glutathione peroxidase and catalase in PC12 cells. Journal of Neurochemistry. 1994 Jun;62(6):2476-2479.
    Sampath, Deepa ; Jackson, George R. ; Werrbach-Perez, Karin ; Perez-Polo, J. Regino. / Effects of nerve growth factor on glutathione peroxidase and catalase in PC12 cells. In: Journal of Neurochemistry. 1994 ; Vol. 62, No. 6. pp. 2476-2479.
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