Effects of nicotine-specific antibodies, Nic311 and Nic-IgG, on the transfer of nicotine across the human placenta

Ilona A. Nekhayeva, Tatiana Nanovskaya, Paul R. Pentel, Dan E. Keyler, Gary Hankins, Mahmoud Ahmed

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The adverse effects of smoking during pregnancy on fetal development are, in part, due to nicotine. These effects may be due to the actions of nicotine in fetal circulation or on placental functions. In pregnant rats, vaccination with a nicotine immunogen reduces the transfer of nicotine from the maternal to fetal circulation. However, extrapolation of these results to pregnant women might not be valid due to the well-recognized differences between human and rat placentas. In the current investigation, the effects of nicotine-specific antibodies on the transfer of nicotine from the maternal to fetal circuit of the dually perfused human placental lobule were determined. Two types of nicotine-specific antibodies were investigated; nicotine-specific mouse monoclonal antibody (Nic311, Kd for nicotine 60 nM) and IgG from rabbits vaccinated with a nicotine immunogen (Nic-IgG, Kd 1.6 nM). Transfer of the antibodies from maternal to fetal circuits was negligible. Both rabbit Nic-IgG and, to a lesser extent, mouse monoclonal Nic311 significantly reduced nicotine transfer from the maternal to fetal circuit as well as the retention of the drug by placental tissue. These effects were mediated by a substantial increase in the protein binding of nicotine and a reduction in the unbound nicotine concentration. Therefore, the data cited in this report suggest that the use of nicotine-specific antibodies might reduce fetal exposure to the drug, and that antibody affinity for nicotine is a key determinant of the extent of nicotine transfer.

Original languageEnglish (US)
Pages (from-to)1664-1672
Number of pages9
JournalBiochemical Pharmacology
Volume70
Issue number11
DOIs
StatePublished - Nov 25 2005

Fingerprint

Nicotine
Placenta
Immunoglobulin G
Antibodies
Mothers
Networks (circuits)
Rats
Rabbits
Antibody Affinity
Fetal Development
Extrapolation
Protein Binding
Pharmaceutical Preparations
Pregnant Women
Vaccination

Keywords

  • Human placenta
  • Nicotine
  • Nicotine-specific antibodies
  • Pregnancy
  • Transplacental transfer

ASJC Scopus subject areas

  • Pharmacology

Cite this

Effects of nicotine-specific antibodies, Nic311 and Nic-IgG, on the transfer of nicotine across the human placenta. / Nekhayeva, Ilona A.; Nanovskaya, Tatiana; Pentel, Paul R.; Keyler, Dan E.; Hankins, Gary; Ahmed, Mahmoud.

In: Biochemical Pharmacology, Vol. 70, No. 11, 25.11.2005, p. 1664-1672.

Research output: Contribution to journalArticle

Nekhayeva, Ilona A. ; Nanovskaya, Tatiana ; Pentel, Paul R. ; Keyler, Dan E. ; Hankins, Gary ; Ahmed, Mahmoud. / Effects of nicotine-specific antibodies, Nic311 and Nic-IgG, on the transfer of nicotine across the human placenta. In: Biochemical Pharmacology. 2005 ; Vol. 70, No. 11. pp. 1664-1672.
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