Effects of Nocodazole on Structures of Calf Brain Tubulin

James C. Lee, Deborah J. Field, Lucy L.Y. Lee

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Abstract

The interaction of nocodazole with calf brain tubulin was studied to determine the effect of such interaction on the structure of tubulin. The effect of nocodazole on the self-association of tubulin was monitored by turbidity measurements and velocity sedimentation. Sedimentation patterns indicate that nocodazole neither induces tubulin to undergo self-association to form higher orders of aggregate nor does it perturb the equilibrium of the reaction leading to the formation of 42S double-ring structures although nocodazole binds to both the tubulin dimers and the polymeric form. Nocodazole does, however, inhibit the in vitro reconstitution The interaction of nocodazole with calf brain tubulin was studied to determine the effect of such interaction on the structure of tubulin. The effect of nocodazole on the self-association of tubulin was monitored by turbidity measurements and velocity sedimentation. Sedimentation patterns indicate that nocodazole neither induces tubulin to undergo self-association to form higher orders of aggregate nor does it perturb the equilibrium of the reaction leading to the formation of 42S double-ring structures although nocodazole binds to both the tubulin dimers and the polymeric form. Nocodazole does, however, inhibit the in vitro reconstitution.

Original languageEnglish (US)
Pages (from-to)6209-6215
Number of pages7
JournalBiochemistry
Volume19
Issue number26
DOIs
StatePublished - Feb 1 1980

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ASJC Scopus subject areas

  • Biochemistry

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Lee, J. C., Field, D. J., & Lee, L. L. Y. (1980). Effects of Nocodazole on Structures of Calf Brain Tubulin. Biochemistry, 19(26), 6209-6215. https://doi.org/10.1021/bi00567a041