Effects of nocodazole on structures of calf brain tubulin

James Lee, Deborah J. Field, Lucy L Y Lee

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

The interaction of nocodazole with calf brain tubulin was studied to determine the effect of such interaction on the structure of tubulin. The effect of nocodazole on the self-association of tubulin was monitored by turbidity measurements and velocity sedimentation. Sedimentation patterns indicate that nocodazole neither induces tubulin to undergo self-association to form higher orders of aggregate nor does it perturb the equilibrium of the reaction leading to the formation of 42S double-ring structures although nocodazole binds to both the tubulin dimers and the polymeric form. Nocodazole does, however, inhibit the in vitro reconstitution of microtubules, and the presence of microtubule-associated proteins does not amplify the inhibitory effect of the drug. The conformational changes in tubulin upon binding of nocodazole were monitored by differential spectroscopy, circular dichroism, fluorescence, and chemical modification of sulfhydryl residues. Results from these studies show that the sulfhydryl residues become more accessible to chemical modification. In contrast, the binding of nocodazole does not significantly alter the net environment of tryptophan chromophores. These residues are apparently not all located on the surface of the tubulin molecule and at least some are partially buried.

Original languageEnglish (US)
Pages (from-to)6209-6215
Number of pages7
JournalBiochemistry
Volume19
Issue number26
StatePublished - 1980
Externally publishedYes

Fingerprint

Nocodazole
Tubulin
Brain
Chemical modification
Sedimentation
Circular dichroism spectroscopy
Microtubule-Associated Proteins
Turbidity
Chromophores
Circular Dichroism
Microtubules
Tryptophan
Dimers
Spectrum Analysis
Fluorescence
Molecules
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Biochemistry

Cite this

Lee, J., Field, D. J., & Lee, L. L. Y. (1980). Effects of nocodazole on structures of calf brain tubulin. Biochemistry, 19(26), 6209-6215.

Effects of nocodazole on structures of calf brain tubulin. / Lee, James; Field, Deborah J.; Lee, Lucy L Y.

In: Biochemistry, Vol. 19, No. 26, 1980, p. 6209-6215.

Research output: Contribution to journalArticle

Lee, J, Field, DJ & Lee, LLY 1980, 'Effects of nocodazole on structures of calf brain tubulin', Biochemistry, vol. 19, no. 26, pp. 6209-6215.
Lee J, Field DJ, Lee LLY. Effects of nocodazole on structures of calf brain tubulin. Biochemistry. 1980;19(26):6209-6215.
Lee, James ; Field, Deborah J. ; Lee, Lucy L Y. / Effects of nocodazole on structures of calf brain tubulin. In: Biochemistry. 1980 ; Vol. 19, No. 26. pp. 6209-6215.
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