Effects of pancreastatin and chromogranin A on insulin release stimulated by various insulinotropic agents

Jin Ishizuka, Kazuhiko Tatemoto, David V. Cohn, James C. Thompson, George H. Greeley

    Research output: Contribution to journalArticle

    12 Scopus citations

    Abstract

    The effects of porcine pancreastatin on insulin release stimulated by insulinotropic agents, glucagon, cholecystokinin-octapeptide (CCK-8), gastric inhibitory polypeptide (GIP) and l-arginine, were compared to those of bovine chromogranin A (CGA) using the isolated perfused rat pancreas. Pancreastatin significantly potentiated glucagon-stimulated insulin release (first phase: 12.5 ± 0.9 ng/8 min; second phase: 34.5 ± 1.6 ng/25 min in controls; 16.5 ± 1.1 ng/8 min and 44.0 ± 2.2 ng/25 min in pancreastatin group), whereas CGA was ineffective. The first phase of l-arginine-stimulated insulin release was also potentiated by pancreastatin (6.9 ± 0.5 ng/5 min in controls, 8.4 ± 0.6 ng/5 min in pancreastatin group), but not by CGA. Pancreastatin did not affect CCK-8 or GIP-stimulated insulin release. Similarly, CGA did not affect insulin release stimulated by CCK-8 or GIP. These findings suggest that pancreastatin stimulates insulin release in the presence of glucagon. Because pancreastatin can have multiple effects on insulin release, which are dependent upon the local concentration of insulin effectors, pancreastatin may participate in the fine tuning of insulin release from B cells.

    Original languageEnglish (US)
    Pages (from-to)25-32
    Number of pages8
    JournalRegulatory Peptides
    Volume34
    Issue number1
    DOIs
    StatePublished - Jun 11 1991

    Keywords

    • CCK-8
    • GIP
    • Glucagon
    • Rat pancreas
    • l-Arginine

    ASJC Scopus subject areas

    • Biochemistry
    • Physiology
    • Endocrinology
    • Clinical Biochemistry
    • Cellular and Molecular Neuroscience

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