Effects of phencyclidine on the release and synthesis of newly formed dopamine

T. W. Vickroy, K. M. Johnson

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The effect of phencyclidine (PCP) on the release and synthesis of [3H]-dopamine ([3H]-DA) newly synthesized from [3,5-3H]-l-tyrosine was studied under both basal and potassium-stimulated conditions in slices from the rat striatum. Phencyclidine (3-100 μM) stimulated the release of [3H]-DA during superfusion with low level potassium (4.5 mM) buffer, but had no effect on the release of [3H]-DA elicited by superfusion with buffer containing 40 mM potassium. On the other hand, phenycyclidine had no effect (except at 100 μm) on the spontaneous release of [3H]-H2O (formed as a result of the hydroxylation of [3,5-3H]-l-tyrosine), but inhibited the increase in formation of [3H]-H2O normally associated with potassium-induced depolarization. These data are discussed in relation to results obtained with phencyclidine on other in vitro and in vivo models of dopaminergic function.

Original languageEnglish (US)
Pages (from-to)839-842
Number of pages4
JournalNeuropharmacology
Volume22
Issue number7
DOIs
StatePublished - 1983

Fingerprint

Phencyclidine
Dopamine
Potassium
Tyrosine
Buffers
Hydroxylation

Keywords

  • dopamine synthesis
  • phencyclidine
  • release
  • striatum

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Drug Discovery
  • Pharmacology

Cite this

Effects of phencyclidine on the release and synthesis of newly formed dopamine. / Vickroy, T. W.; Johnson, K. M.

In: Neuropharmacology, Vol. 22, No. 7, 1983, p. 839-842.

Research output: Contribution to journalArticle

Vickroy, T. W. ; Johnson, K. M. / Effects of phencyclidine on the release and synthesis of newly formed dopamine. In: Neuropharmacology. 1983 ; Vol. 22, No. 7. pp. 839-842.
@article{27196decb31b48abadfb50736af15914,
title = "Effects of phencyclidine on the release and synthesis of newly formed dopamine",
abstract = "The effect of phencyclidine (PCP) on the release and synthesis of [3H]-dopamine ([3H]-DA) newly synthesized from [3,5-3H]-l-tyrosine was studied under both basal and potassium-stimulated conditions in slices from the rat striatum. Phencyclidine (3-100 μM) stimulated the release of [3H]-DA during superfusion with low level potassium (4.5 mM) buffer, but had no effect on the release of [3H]-DA elicited by superfusion with buffer containing 40 mM potassium. On the other hand, phenycyclidine had no effect (except at 100 μm) on the spontaneous release of [3H]-H2O (formed as a result of the hydroxylation of [3,5-3H]-l-tyrosine), but inhibited the increase in formation of [3H]-H2O normally associated with potassium-induced depolarization. These data are discussed in relation to results obtained with phencyclidine on other in vitro and in vivo models of dopaminergic function.",
keywords = "dopamine synthesis, phencyclidine, release, striatum",
author = "Vickroy, {T. W.} and Johnson, {K. M.}",
year = "1983",
doi = "10.1016/0028-3908(83)90129-6",
language = "English (US)",
volume = "22",
pages = "839--842",
journal = "Neuropharmacology",
issn = "0028-3908",
publisher = "Elsevier Limited",
number = "7",

}

TY - JOUR

T1 - Effects of phencyclidine on the release and synthesis of newly formed dopamine

AU - Vickroy, T. W.

AU - Johnson, K. M.

PY - 1983

Y1 - 1983

N2 - The effect of phencyclidine (PCP) on the release and synthesis of [3H]-dopamine ([3H]-DA) newly synthesized from [3,5-3H]-l-tyrosine was studied under both basal and potassium-stimulated conditions in slices from the rat striatum. Phencyclidine (3-100 μM) stimulated the release of [3H]-DA during superfusion with low level potassium (4.5 mM) buffer, but had no effect on the release of [3H]-DA elicited by superfusion with buffer containing 40 mM potassium. On the other hand, phenycyclidine had no effect (except at 100 μm) on the spontaneous release of [3H]-H2O (formed as a result of the hydroxylation of [3,5-3H]-l-tyrosine), but inhibited the increase in formation of [3H]-H2O normally associated with potassium-induced depolarization. These data are discussed in relation to results obtained with phencyclidine on other in vitro and in vivo models of dopaminergic function.

AB - The effect of phencyclidine (PCP) on the release and synthesis of [3H]-dopamine ([3H]-DA) newly synthesized from [3,5-3H]-l-tyrosine was studied under both basal and potassium-stimulated conditions in slices from the rat striatum. Phencyclidine (3-100 μM) stimulated the release of [3H]-DA during superfusion with low level potassium (4.5 mM) buffer, but had no effect on the release of [3H]-DA elicited by superfusion with buffer containing 40 mM potassium. On the other hand, phenycyclidine had no effect (except at 100 μm) on the spontaneous release of [3H]-H2O (formed as a result of the hydroxylation of [3,5-3H]-l-tyrosine), but inhibited the increase in formation of [3H]-H2O normally associated with potassium-induced depolarization. These data are discussed in relation to results obtained with phencyclidine on other in vitro and in vivo models of dopaminergic function.

KW - dopamine synthesis

KW - phencyclidine

KW - release

KW - striatum

UR - http://www.scopus.com/inward/record.url?scp=0020507408&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020507408&partnerID=8YFLogxK

U2 - 10.1016/0028-3908(83)90129-6

DO - 10.1016/0028-3908(83)90129-6

M3 - Article

VL - 22

SP - 839

EP - 842

JO - Neuropharmacology

JF - Neuropharmacology

SN - 0028-3908

IS - 7

ER -