Effects of physiological leptin administration on markers of inflammation, platelet activation, and platelet aggregation during caloric deprivation

Bridget Canavan, Raneem O. Salem, Sunita Schurgin, Polyxeni Koutkia, Izabella Lipinska, Michael Laposata, Steven Grinspoon

Research output: Contribution to journalArticle

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Abstract

Context: Leptin is a nutritionally regulated adipocyte-derived cytokine. Previous studies in obese patients have demonstrated increased inflammatory markers and increased platelet aggregation in association with leptin. However, the effects of leptin administration on markers of inflammation and platelet aggregation in a human model of undernutrition have not previously been studied. Objective: The objective of the study was to investigate markers of inflammation, platelet activation, and platelet aggregation in a model of caloric deprivation and increased leptin sensitivity. Design: This study was a randomized, placebo-controlled study conducted between November 2002 and November 2003. Setting: The study was conducted at an inpatient care setting at the General Clinical Research Center. Participants: Twenty healthy, young (18-35 yr old), normal-weight (body mass index, 20-26 kg/m2) women were recruited from local advertisements. No subjects withdrew due to adverse effects. Intervention: The effects of physiological recombinant methionyl human leptin or identical placebo administration were investigated over a 4-d fast. Main Outcome Measures: The primary outcome measures for this study were C-reactive protein (CRP) and indices of platelet activity. Results: Leptin administration prevented the fasting-induced decline in leptin (P < 0.05 vs. placebo at each time point). Leptin administration increased CRP (6.3 ± 2.4 vs. 0.7 ± 0.3 mg/liter; P = 0.04), circulating P-selectin (11.6 ± 10.2 vs. -28.9 ± 15.6 ng/ml; P = 0.04), and induction of platelet aggregation (5.8 ± 2.6 vs. -2.7 ± 2.9%, P = 0.04, percent maximum platelet aggregation) relative to placebo administration (change in leptin vs. change in placebo, respectively, for each variable). Leptin tended to increase serum amyloid A [0.1 ± 0.2 vs. -0.3 ± 0.1 log 10 (ng/ml); P = 0.07], and the changes in serum amyloid A and CRP were highly correlated (r = 0.83; P < 0.0001). No changes in TNFα, IL-6, IL-10, plasminogen activator inhibitor-1, haptoglobin, intercellular adhesion molecule, or vascular cell adhesion molecule were seen between the groups. Conclusions: Our data provide evidence that physiological leptin administration stimulates inflammatory and platelet responses in humans during caloric deprivation.

Original languageEnglish (US)
Pages (from-to)5779-5785
Number of pages7
JournalJournal of Clinical Endocrinology and Metabolism
Volume90
Issue number10
DOIs
StatePublished - Oct 2005
Externally publishedYes

Fingerprint

Platelet Activation
Platelets
Leptin
Platelet Aggregation
Agglomeration
Chemical activation
Inflammation
Placebos
C-Reactive Protein
Serum Amyloid A Protein
Outcome Assessment (Health Care)
Blood Platelets
Haptoglobins
P-Selectin
Vascular Cell Adhesion Molecule-1
Plasminogen Activator Inhibitor 1
Cell Adhesion Molecules
Adipocytes
Malnutrition
Interleukin-10

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Effects of physiological leptin administration on markers of inflammation, platelet activation, and platelet aggregation during caloric deprivation. / Canavan, Bridget; Salem, Raneem O.; Schurgin, Sunita; Koutkia, Polyxeni; Lipinska, Izabella; Laposata, Michael; Grinspoon, Steven.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 90, No. 10, 10.2005, p. 5779-5785.

Research output: Contribution to journalArticle

Canavan, Bridget ; Salem, Raneem O. ; Schurgin, Sunita ; Koutkia, Polyxeni ; Lipinska, Izabella ; Laposata, Michael ; Grinspoon, Steven. / Effects of physiological leptin administration on markers of inflammation, platelet activation, and platelet aggregation during caloric deprivation. In: Journal of Clinical Endocrinology and Metabolism. 2005 ; Vol. 90, No. 10. pp. 5779-5785.
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abstract = "Context: Leptin is a nutritionally regulated adipocyte-derived cytokine. Previous studies in obese patients have demonstrated increased inflammatory markers and increased platelet aggregation in association with leptin. However, the effects of leptin administration on markers of inflammation and platelet aggregation in a human model of undernutrition have not previously been studied. Objective: The objective of the study was to investigate markers of inflammation, platelet activation, and platelet aggregation in a model of caloric deprivation and increased leptin sensitivity. Design: This study was a randomized, placebo-controlled study conducted between November 2002 and November 2003. Setting: The study was conducted at an inpatient care setting at the General Clinical Research Center. Participants: Twenty healthy, young (18-35 yr old), normal-weight (body mass index, 20-26 kg/m2) women were recruited from local advertisements. No subjects withdrew due to adverse effects. Intervention: The effects of physiological recombinant methionyl human leptin or identical placebo administration were investigated over a 4-d fast. Main Outcome Measures: The primary outcome measures for this study were C-reactive protein (CRP) and indices of platelet activity. Results: Leptin administration prevented the fasting-induced decline in leptin (P < 0.05 vs. placebo at each time point). Leptin administration increased CRP (6.3 ± 2.4 vs. 0.7 ± 0.3 mg/liter; P = 0.04), circulating P-selectin (11.6 ± 10.2 vs. -28.9 ± 15.6 ng/ml; P = 0.04), and induction of platelet aggregation (5.8 ± 2.6 vs. -2.7 ± 2.9{\%}, P = 0.04, percent maximum platelet aggregation) relative to placebo administration (change in leptin vs. change in placebo, respectively, for each variable). Leptin tended to increase serum amyloid A [0.1 ± 0.2 vs. -0.3 ± 0.1 log 10 (ng/ml); P = 0.07], and the changes in serum amyloid A and CRP were highly correlated (r = 0.83; P < 0.0001). No changes in TNFα, IL-6, IL-10, plasminogen activator inhibitor-1, haptoglobin, intercellular adhesion molecule, or vascular cell adhesion molecule were seen between the groups. Conclusions: Our data provide evidence that physiological leptin administration stimulates inflammatory and platelet responses in humans during caloric deprivation.",
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AU - Canavan, Bridget

AU - Salem, Raneem O.

AU - Schurgin, Sunita

AU - Koutkia, Polyxeni

AU - Lipinska, Izabella

AU - Laposata, Michael

AU - Grinspoon, Steven

PY - 2005/10

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N2 - Context: Leptin is a nutritionally regulated adipocyte-derived cytokine. Previous studies in obese patients have demonstrated increased inflammatory markers and increased platelet aggregation in association with leptin. However, the effects of leptin administration on markers of inflammation and platelet aggregation in a human model of undernutrition have not previously been studied. Objective: The objective of the study was to investigate markers of inflammation, platelet activation, and platelet aggregation in a model of caloric deprivation and increased leptin sensitivity. Design: This study was a randomized, placebo-controlled study conducted between November 2002 and November 2003. Setting: The study was conducted at an inpatient care setting at the General Clinical Research Center. Participants: Twenty healthy, young (18-35 yr old), normal-weight (body mass index, 20-26 kg/m2) women were recruited from local advertisements. No subjects withdrew due to adverse effects. Intervention: The effects of physiological recombinant methionyl human leptin or identical placebo administration were investigated over a 4-d fast. Main Outcome Measures: The primary outcome measures for this study were C-reactive protein (CRP) and indices of platelet activity. Results: Leptin administration prevented the fasting-induced decline in leptin (P < 0.05 vs. placebo at each time point). Leptin administration increased CRP (6.3 ± 2.4 vs. 0.7 ± 0.3 mg/liter; P = 0.04), circulating P-selectin (11.6 ± 10.2 vs. -28.9 ± 15.6 ng/ml; P = 0.04), and induction of platelet aggregation (5.8 ± 2.6 vs. -2.7 ± 2.9%, P = 0.04, percent maximum platelet aggregation) relative to placebo administration (change in leptin vs. change in placebo, respectively, for each variable). Leptin tended to increase serum amyloid A [0.1 ± 0.2 vs. -0.3 ± 0.1 log 10 (ng/ml); P = 0.07], and the changes in serum amyloid A and CRP were highly correlated (r = 0.83; P < 0.0001). No changes in TNFα, IL-6, IL-10, plasminogen activator inhibitor-1, haptoglobin, intercellular adhesion molecule, or vascular cell adhesion molecule were seen between the groups. Conclusions: Our data provide evidence that physiological leptin administration stimulates inflammatory and platelet responses in humans during caloric deprivation.

AB - Context: Leptin is a nutritionally regulated adipocyte-derived cytokine. Previous studies in obese patients have demonstrated increased inflammatory markers and increased platelet aggregation in association with leptin. However, the effects of leptin administration on markers of inflammation and platelet aggregation in a human model of undernutrition have not previously been studied. Objective: The objective of the study was to investigate markers of inflammation, platelet activation, and platelet aggregation in a model of caloric deprivation and increased leptin sensitivity. Design: This study was a randomized, placebo-controlled study conducted between November 2002 and November 2003. Setting: The study was conducted at an inpatient care setting at the General Clinical Research Center. Participants: Twenty healthy, young (18-35 yr old), normal-weight (body mass index, 20-26 kg/m2) women were recruited from local advertisements. No subjects withdrew due to adverse effects. Intervention: The effects of physiological recombinant methionyl human leptin or identical placebo administration were investigated over a 4-d fast. Main Outcome Measures: The primary outcome measures for this study were C-reactive protein (CRP) and indices of platelet activity. Results: Leptin administration prevented the fasting-induced decline in leptin (P < 0.05 vs. placebo at each time point). Leptin administration increased CRP (6.3 ± 2.4 vs. 0.7 ± 0.3 mg/liter; P = 0.04), circulating P-selectin (11.6 ± 10.2 vs. -28.9 ± 15.6 ng/ml; P = 0.04), and induction of platelet aggregation (5.8 ± 2.6 vs. -2.7 ± 2.9%, P = 0.04, percent maximum platelet aggregation) relative to placebo administration (change in leptin vs. change in placebo, respectively, for each variable). Leptin tended to increase serum amyloid A [0.1 ± 0.2 vs. -0.3 ± 0.1 log 10 (ng/ml); P = 0.07], and the changes in serum amyloid A and CRP were highly correlated (r = 0.83; P < 0.0001). No changes in TNFα, IL-6, IL-10, plasminogen activator inhibitor-1, haptoglobin, intercellular adhesion molecule, or vascular cell adhesion molecule were seen between the groups. Conclusions: Our data provide evidence that physiological leptin administration stimulates inflammatory and platelet responses in humans during caloric deprivation.

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