Effects of progesterone treatment on expression of genes involved in uterine quiescence

Melvyn S. Soloff, Yow Jiun Jeng, Michael G. Izban, Mala Sinha, Bruce A. Luxon, Susan J. Stamnes, Sarah K. England

    Research output: Contribution to journalArticle

    26 Citations (Scopus)

    Abstract

    An important action of progesterone during pregnancy is to maintain the uterus in a quiescent state and thereby prevent preterm labor. The causes of preterm labor are not well understood, so progesterone action on the myometrium can provide clues about the processes that keep the uterus from contracting prematurely. Accordingly, we have carried out Affymetrix GeneChip analysis of progesterone effects on gene expression in immortalized human myometrial cells cultured from a patient near the end of pregnancy. Progesterone appears to inhibit uterine excitability by a number of mechanisms, including increased expression of calcium and voltage-operated K+ channels, which dampens the electrical activity of the myometrial cell, downregulation of agents, and receptors involved in myometrial contraction, reduction in cell signal components that lead to increased intracellular Ca2+ concentrations in response to contractile stimuli, and downregulation of proteins involved in the cross-linking of actin and myosin filaments to produce uterine contractions.

    Original languageEnglish (US)
    Pages (from-to)781-797
    Number of pages17
    JournalReproductive Sciences
    Volume18
    Issue number8
    DOIs
    StatePublished - Aug 2011

    Fingerprint

    Progesterone
    Gene Expression
    Uterine Contraction
    Premature Obstetric Labor
    Uterus
    Down-Regulation
    Pregnancy
    Myometrium
    Cellular Structures
    Therapeutics
    Myosins
    Actin Cytoskeleton
    Cultured Cells
    Calcium
    Proteins

    Keywords

    • estrogen
    • gene array
    • myometrium
    • progesterrone
    • quantitative PCR
    • quiescence

    ASJC Scopus subject areas

    • Obstetrics and Gynecology

    Cite this

    Soloff, M. S., Jeng, Y. J., Izban, M. G., Sinha, M., Luxon, B. A., Stamnes, S. J., & England, S. K. (2011). Effects of progesterone treatment on expression of genes involved in uterine quiescence. Reproductive Sciences, 18(8), 781-797. https://doi.org/10.1177/1933719111398150

    Effects of progesterone treatment on expression of genes involved in uterine quiescence. / Soloff, Melvyn S.; Jeng, Yow Jiun; Izban, Michael G.; Sinha, Mala; Luxon, Bruce A.; Stamnes, Susan J.; England, Sarah K.

    In: Reproductive Sciences, Vol. 18, No. 8, 08.2011, p. 781-797.

    Research output: Contribution to journalArticle

    Soloff, MS, Jeng, YJ, Izban, MG, Sinha, M, Luxon, BA, Stamnes, SJ & England, SK 2011, 'Effects of progesterone treatment on expression of genes involved in uterine quiescence', Reproductive Sciences, vol. 18, no. 8, pp. 781-797. https://doi.org/10.1177/1933719111398150
    Soloff, Melvyn S. ; Jeng, Yow Jiun ; Izban, Michael G. ; Sinha, Mala ; Luxon, Bruce A. ; Stamnes, Susan J. ; England, Sarah K. / Effects of progesterone treatment on expression of genes involved in uterine quiescence. In: Reproductive Sciences. 2011 ; Vol. 18, No. 8. pp. 781-797.
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