Abstract
Our laboratory as well as others has previously noted that sensitivity of lymphocytes to inhibition by prostaglandin (PG)E2 is lost after overnight preincubation. We now show that the change in sensitivity to PGE2 with preincubation varies depending upon the T-cell subset in the culture. In phytohemaglutinin (PHA)-stimulated cultures of fresh cells, PGE2 inhibits proliferation of T cells as well as OKT4 (+) cells and OKT8(+) cells. In PHA-stimulated cultures of preincubated cells, PGE2 slightly augments proliferation of T cells; however, PGE still inhibits OKT4(+) cells and causes marked augmentation of OKT8(+) cells. Thus the "loss in sensitivity" to PGE2 with preincubation is actually a result of the balancing effect of PGE2 inhibiting OKT4(+) cells and stimulating OKT8(+) cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 373-379 |
| Number of pages | 7 |
| Journal | Cellular Immunology |
| Volume | 70 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jul 1 1982 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cell Biology
- Immunology
Cite this
Effects of prostaglandin E2 and preincubation on lectin-stimulated proliferation of human T cell subsets. / Gualde, Norbert; Goodwin, James.
In: Cellular Immunology, Vol. 70, No. 2, 01.07.1982, p. 373-379.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of prostaglandin E2 and preincubation on lectin-stimulated proliferation of human T cell subsets
AU - Gualde, Norbert
AU - Goodwin, James
PY - 1982/7/1
Y1 - 1982/7/1
N2 - Our laboratory as well as others has previously noted that sensitivity of lymphocytes to inhibition by prostaglandin (PG)E2 is lost after overnight preincubation. We now show that the change in sensitivity to PGE2 with preincubation varies depending upon the T-cell subset in the culture. In phytohemaglutinin (PHA)-stimulated cultures of fresh cells, PGE2 inhibits proliferation of T cells as well as OKT4 (+) cells and OKT8(+) cells. In PHA-stimulated cultures of preincubated cells, PGE2 slightly augments proliferation of T cells; however, PGE still inhibits OKT4(+) cells and causes marked augmentation of OKT8(+) cells. Thus the "loss in sensitivity" to PGE2 with preincubation is actually a result of the balancing effect of PGE2 inhibiting OKT4(+) cells and stimulating OKT8(+) cells.
AB - Our laboratory as well as others has previously noted that sensitivity of lymphocytes to inhibition by prostaglandin (PG)E2 is lost after overnight preincubation. We now show that the change in sensitivity to PGE2 with preincubation varies depending upon the T-cell subset in the culture. In phytohemaglutinin (PHA)-stimulated cultures of fresh cells, PGE2 inhibits proliferation of T cells as well as OKT4 (+) cells and OKT8(+) cells. In PHA-stimulated cultures of preincubated cells, PGE2 slightly augments proliferation of T cells; however, PGE still inhibits OKT4(+) cells and causes marked augmentation of OKT8(+) cells. Thus the "loss in sensitivity" to PGE2 with preincubation is actually a result of the balancing effect of PGE2 inhibiting OKT4(+) cells and stimulating OKT8(+) cells.
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UR - http://www.scopus.com/inward/citedby.url?scp=0020000547&partnerID=8YFLogxK
U2 - 10.1016/0008-8749(82)90339-2
DO - 10.1016/0008-8749(82)90339-2
M3 - Article
C2 - 6215130
AN - SCOPUS:0020000547
VL - 70
SP - 373
EP - 379
JO - Cellular Immunology
JF - Cellular Immunology
SN - 0008-8749
IS - 2
ER -