Effects of recombinant human relaxin on pregnant rat uterine artery and myometrium in vitro

Monica Longo, Venu Jain, Yuri P. Vedernikov, Robert E. Garfield, George Saade, Baha M. Sibai, Chin Chu Lin, Mark I. Evans, Dinesh M. Shah, Andrea G. Witlin, Longo

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

OBJECTIVE: The purpose of this study was to evaluate the effects of recombinant human relaxin on the uterine artery and myometrial contractility in pregnant rats. STUDY DESIGN: Uterine artery and myometrial rings from mid and term pregnant rats were used. Relaxin effect was studied on phenylephrine-induced contraction in the presence or absence of nitric oxide synthase inhibitor, Nω-nitro-I-arginine methyl ester, soluble guanylate cyclase inhibitor, 1H-oxadiazolo-quinoxaline-1-one, or adenylate cyclase inhibitor, SQ-22,536. The myometrial inhibitory effect of relaxin was studied on spontaneous and oxytocin- or protein kinase C activator-induced contractions. RESULTS: Uterine artery relaxation by relaxin was greater at mid pregnancy compared with term. Relaxin effect was decreased by SQ-22,536, 1H-oxadiazolo-quinoxaline-1-one and Nω-nitro-I-arginine methyl ester at mid pregnancy. Relaxin inhibited spontaneous contractions at mid pregnancy but not at term. Relaxin had no effect on oxytocin- or indolactam-V-induced contractions. CONCLUSION: Relaxin effect is mediated by nitric oxide, soluble guanylate cyclase, and adenylate cyclase in mid pregnant uterine artery. Relaxin inhibits spontaneous uterine activity at mid pregnancy. Relaxin effect decreased at term gestation in both tissues.

Original languageEnglish (US)
Pages (from-to)1468-1476
Number of pages9
JournalAmerican Journal of Obstetrics and Gynecology
Volume188
Issue number6
DOIs
StatePublished - Jun 1 2003

Fingerprint

Relaxin
Uterine Artery
Myometrium
Pregnancy
Quinoxalines
Oxytocin
In Vitro Techniques
Phenylephrine
Adenylyl Cyclases
Nitric Oxide Synthase
Protein Kinase C
Nitric Oxide

Keywords

  • Myometrium
  • Nitric oxide
  • Pregnant rat
  • Relaxin
  • Uterine artery

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Effects of recombinant human relaxin on pregnant rat uterine artery and myometrium in vitro. / Longo, Monica; Jain, Venu; Vedernikov, Yuri P.; Garfield, Robert E.; Saade, George; Sibai, Baha M.; Lin, Chin Chu; Evans, Mark I.; Shah, Dinesh M.; Witlin, Andrea G.; Longo.

In: American Journal of Obstetrics and Gynecology, Vol. 188, No. 6, 01.06.2003, p. 1468-1476.

Research output: Contribution to journalArticle

Longo, M, Jain, V, Vedernikov, YP, Garfield, RE, Saade, G, Sibai, BM, Lin, CC, Evans, MI, Shah, DM, Witlin, AG & Longo 2003, 'Effects of recombinant human relaxin on pregnant rat uterine artery and myometrium in vitro', American Journal of Obstetrics and Gynecology, vol. 188, no. 6, pp. 1468-1476. https://doi.org/10.1067/mob.2003.454
Longo, Monica ; Jain, Venu ; Vedernikov, Yuri P. ; Garfield, Robert E. ; Saade, George ; Sibai, Baha M. ; Lin, Chin Chu ; Evans, Mark I. ; Shah, Dinesh M. ; Witlin, Andrea G. ; Longo. / Effects of recombinant human relaxin on pregnant rat uterine artery and myometrium in vitro. In: American Journal of Obstetrics and Gynecology. 2003 ; Vol. 188, No. 6. pp. 1468-1476.
@article{40cdd15192c84c99beef5f0cb0ed7789,
title = "Effects of recombinant human relaxin on pregnant rat uterine artery and myometrium in vitro",
abstract = "OBJECTIVE: The purpose of this study was to evaluate the effects of recombinant human relaxin on the uterine artery and myometrial contractility in pregnant rats. STUDY DESIGN: Uterine artery and myometrial rings from mid and term pregnant rats were used. Relaxin effect was studied on phenylephrine-induced contraction in the presence or absence of nitric oxide synthase inhibitor, Nω-nitro-I-arginine methyl ester, soluble guanylate cyclase inhibitor, 1H-oxadiazolo-quinoxaline-1-one, or adenylate cyclase inhibitor, SQ-22,536. The myometrial inhibitory effect of relaxin was studied on spontaneous and oxytocin- or protein kinase C activator-induced contractions. RESULTS: Uterine artery relaxation by relaxin was greater at mid pregnancy compared with term. Relaxin effect was decreased by SQ-22,536, 1H-oxadiazolo-quinoxaline-1-one and Nω-nitro-I-arginine methyl ester at mid pregnancy. Relaxin inhibited spontaneous contractions at mid pregnancy but not at term. Relaxin had no effect on oxytocin- or indolactam-V-induced contractions. CONCLUSION: Relaxin effect is mediated by nitric oxide, soluble guanylate cyclase, and adenylate cyclase in mid pregnant uterine artery. Relaxin inhibits spontaneous uterine activity at mid pregnancy. Relaxin effect decreased at term gestation in both tissues.",
keywords = "Myometrium, Nitric oxide, Pregnant rat, Relaxin, Uterine artery",
author = "Monica Longo and Venu Jain and Vedernikov, {Yuri P.} and Garfield, {Robert E.} and George Saade and Sibai, {Baha M.} and Lin, {Chin Chu} and Evans, {Mark I.} and Shah, {Dinesh M.} and Witlin, {Andrea G.} and Longo",
year = "2003",
month = "6",
day = "1",
doi = "10.1067/mob.2003.454",
language = "English (US)",
volume = "188",
pages = "1468--1476",
journal = "American Journal of Obstetrics and Gynecology",
issn = "0002-9378",
publisher = "Mosby Inc.",
number = "6",

}

TY - JOUR

T1 - Effects of recombinant human relaxin on pregnant rat uterine artery and myometrium in vitro

AU - Longo, Monica

AU - Jain, Venu

AU - Vedernikov, Yuri P.

AU - Garfield, Robert E.

AU - Saade, George

AU - Sibai, Baha M.

AU - Lin, Chin Chu

AU - Evans, Mark I.

AU - Shah, Dinesh M.

AU - Witlin, Andrea G.

AU - Longo,

PY - 2003/6/1

Y1 - 2003/6/1

N2 - OBJECTIVE: The purpose of this study was to evaluate the effects of recombinant human relaxin on the uterine artery and myometrial contractility in pregnant rats. STUDY DESIGN: Uterine artery and myometrial rings from mid and term pregnant rats were used. Relaxin effect was studied on phenylephrine-induced contraction in the presence or absence of nitric oxide synthase inhibitor, Nω-nitro-I-arginine methyl ester, soluble guanylate cyclase inhibitor, 1H-oxadiazolo-quinoxaline-1-one, or adenylate cyclase inhibitor, SQ-22,536. The myometrial inhibitory effect of relaxin was studied on spontaneous and oxytocin- or protein kinase C activator-induced contractions. RESULTS: Uterine artery relaxation by relaxin was greater at mid pregnancy compared with term. Relaxin effect was decreased by SQ-22,536, 1H-oxadiazolo-quinoxaline-1-one and Nω-nitro-I-arginine methyl ester at mid pregnancy. Relaxin inhibited spontaneous contractions at mid pregnancy but not at term. Relaxin had no effect on oxytocin- or indolactam-V-induced contractions. CONCLUSION: Relaxin effect is mediated by nitric oxide, soluble guanylate cyclase, and adenylate cyclase in mid pregnant uterine artery. Relaxin inhibits spontaneous uterine activity at mid pregnancy. Relaxin effect decreased at term gestation in both tissues.

AB - OBJECTIVE: The purpose of this study was to evaluate the effects of recombinant human relaxin on the uterine artery and myometrial contractility in pregnant rats. STUDY DESIGN: Uterine artery and myometrial rings from mid and term pregnant rats were used. Relaxin effect was studied on phenylephrine-induced contraction in the presence or absence of nitric oxide synthase inhibitor, Nω-nitro-I-arginine methyl ester, soluble guanylate cyclase inhibitor, 1H-oxadiazolo-quinoxaline-1-one, or adenylate cyclase inhibitor, SQ-22,536. The myometrial inhibitory effect of relaxin was studied on spontaneous and oxytocin- or protein kinase C activator-induced contractions. RESULTS: Uterine artery relaxation by relaxin was greater at mid pregnancy compared with term. Relaxin effect was decreased by SQ-22,536, 1H-oxadiazolo-quinoxaline-1-one and Nω-nitro-I-arginine methyl ester at mid pregnancy. Relaxin inhibited spontaneous contractions at mid pregnancy but not at term. Relaxin had no effect on oxytocin- or indolactam-V-induced contractions. CONCLUSION: Relaxin effect is mediated by nitric oxide, soluble guanylate cyclase, and adenylate cyclase in mid pregnant uterine artery. Relaxin inhibits spontaneous uterine activity at mid pregnancy. Relaxin effect decreased at term gestation in both tissues.

KW - Myometrium

KW - Nitric oxide

KW - Pregnant rat

KW - Relaxin

KW - Uterine artery

UR - http://www.scopus.com/inward/record.url?scp=0038000674&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038000674&partnerID=8YFLogxK

U2 - 10.1067/mob.2003.454

DO - 10.1067/mob.2003.454

M3 - Article

VL - 188

SP - 1468

EP - 1476

JO - American Journal of Obstetrics and Gynecology

JF - American Journal of Obstetrics and Gynecology

SN - 0002-9378

IS - 6

ER -