Effects of selected vasoconstrictor agonists on isolated omental artery from premenopausal nonpregnant women and from normal and preeclamptic pregnant women

M. A. Belfort, George Saade, M. Suresh, W. Kramer, Y. P. Vedernikov

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

OBJECTIVE: Our purpose was to compare the responsiveness of omental resistance arteries from nonpregnant women and from normotensive and preeclamptic pregnant women to selected contractile agonists. STUDY DESIGN: Omental artery rings with intact endothelium from normotensive premenopausal nonpregnant women and from normal and preeclamptic pregnant women were mounted in Krebs-bicarbonate solution in organ baths for isometric tension recording. After the presence of endothelium was confirmed, cumulative concentrations of norepinephrine, serotonin, U46619, and endothelin-1 were added. Concentration-response curves were constructed and expressed as percentage of a reference 60 mmol/L potassium chloride contraction. Data analysis was by repeated-measures analysis of variance, Newman-Keuls test, and paired or unpaired Student t test, as appropriate. Statistical significance was by two-tailed p < 0.05. RESULTS: Endothelin-1 and U46619 increased tension similarly in all three groups. Norepinephrine increased tension in nonpregnant vessels to a greater extent than in either preeclamptic or pregnant vessels (nonpregnant 114.3 ± 5.42% vs pregnant 65.2% ± 10.5%, P < 0.05). Nonpregnant omental artery developed significantly greater tension than did pregnant tissue at three concentrations of norepinephrine (10-5 mol/L, 3 x 10-5 mol/L, 10-4 mol/L), and preeclamptic vessels developed more tension than that from normal pregnant vessels at 3 x 10-6 mol/L (p = 0.06) and 10-3 mol/L (p < 0.05). There was a negligible change in tension with increasing concentrations of serotonin in the vessels from nonpregnant women; serotonin-induced contraction in the omental arteries from normotensive pregnant women and preeclamptic patients was <6% of the potassium chloride reference contraction, but this was significantly (p < 0.05) different from that of the nonpregnant women. CONCLUSIONS: Omental artery segments from nonpregnant, normotensive pregnant and preeclamptic women contract similarly to endothelin-1 and U46619 but exhibit variable responses to norepinephrine and serotonin.

Original languageEnglish (US)
Pages (from-to)687-693
Number of pages7
JournalAmerican Journal of Obstetrics and Gynecology
Volume174
Issue number2
DOIs
StatePublished - 1996
Externally publishedYes

Fingerprint

Vasoconstrictor Agents
Pregnant Women
Arteries
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
Serotonin
Norepinephrine
Endothelin-1
Potassium Chloride
Endothelium
Bicarbonates
Baths
Analysis of Variance
Students

Keywords

  • omental artery
  • preeclampsia
  • Vasoconstrictor agonist

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Effects of selected vasoconstrictor agonists on isolated omental artery from premenopausal nonpregnant women and from normal and preeclamptic pregnant women. / Belfort, M. A.; Saade, George; Suresh, M.; Kramer, W.; Vedernikov, Y. P.

In: American Journal of Obstetrics and Gynecology, Vol. 174, No. 2, 1996, p. 687-693.

Research output: Contribution to journalArticle

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abstract = "OBJECTIVE: Our purpose was to compare the responsiveness of omental resistance arteries from nonpregnant women and from normotensive and preeclamptic pregnant women to selected contractile agonists. STUDY DESIGN: Omental artery rings with intact endothelium from normotensive premenopausal nonpregnant women and from normal and preeclamptic pregnant women were mounted in Krebs-bicarbonate solution in organ baths for isometric tension recording. After the presence of endothelium was confirmed, cumulative concentrations of norepinephrine, serotonin, U46619, and endothelin-1 were added. Concentration-response curves were constructed and expressed as percentage of a reference 60 mmol/L potassium chloride contraction. Data analysis was by repeated-measures analysis of variance, Newman-Keuls test, and paired or unpaired Student t test, as appropriate. Statistical significance was by two-tailed p < 0.05. RESULTS: Endothelin-1 and U46619 increased tension similarly in all three groups. Norepinephrine increased tension in nonpregnant vessels to a greater extent than in either preeclamptic or pregnant vessels (nonpregnant 114.3 ± 5.42{\%} vs pregnant 65.2{\%} ± 10.5{\%}, P < 0.05). Nonpregnant omental artery developed significantly greater tension than did pregnant tissue at three concentrations of norepinephrine (10-5 mol/L, 3 x 10-5 mol/L, 10-4 mol/L), and preeclamptic vessels developed more tension than that from normal pregnant vessels at 3 x 10-6 mol/L (p = 0.06) and 10-3 mol/L (p < 0.05). There was a negligible change in tension with increasing concentrations of serotonin in the vessels from nonpregnant women; serotonin-induced contraction in the omental arteries from normotensive pregnant women and preeclamptic patients was <6{\%} of the potassium chloride reference contraction, but this was significantly (p < 0.05) different from that of the nonpregnant women. CONCLUSIONS: Omental artery segments from nonpregnant, normotensive pregnant and preeclamptic women contract similarly to endothelin-1 and U46619 but exhibit variable responses to norepinephrine and serotonin.",
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T1 - Effects of selected vasoconstrictor agonists on isolated omental artery from premenopausal nonpregnant women and from normal and preeclamptic pregnant women

AU - Belfort, M. A.

AU - Saade, George

AU - Suresh, M.

AU - Kramer, W.

AU - Vedernikov, Y. P.

PY - 1996

Y1 - 1996

N2 - OBJECTIVE: Our purpose was to compare the responsiveness of omental resistance arteries from nonpregnant women and from normotensive and preeclamptic pregnant women to selected contractile agonists. STUDY DESIGN: Omental artery rings with intact endothelium from normotensive premenopausal nonpregnant women and from normal and preeclamptic pregnant women were mounted in Krebs-bicarbonate solution in organ baths for isometric tension recording. After the presence of endothelium was confirmed, cumulative concentrations of norepinephrine, serotonin, U46619, and endothelin-1 were added. Concentration-response curves were constructed and expressed as percentage of a reference 60 mmol/L potassium chloride contraction. Data analysis was by repeated-measures analysis of variance, Newman-Keuls test, and paired or unpaired Student t test, as appropriate. Statistical significance was by two-tailed p < 0.05. RESULTS: Endothelin-1 and U46619 increased tension similarly in all three groups. Norepinephrine increased tension in nonpregnant vessels to a greater extent than in either preeclamptic or pregnant vessels (nonpregnant 114.3 ± 5.42% vs pregnant 65.2% ± 10.5%, P < 0.05). Nonpregnant omental artery developed significantly greater tension than did pregnant tissue at three concentrations of norepinephrine (10-5 mol/L, 3 x 10-5 mol/L, 10-4 mol/L), and preeclamptic vessels developed more tension than that from normal pregnant vessels at 3 x 10-6 mol/L (p = 0.06) and 10-3 mol/L (p < 0.05). There was a negligible change in tension with increasing concentrations of serotonin in the vessels from nonpregnant women; serotonin-induced contraction in the omental arteries from normotensive pregnant women and preeclamptic patients was <6% of the potassium chloride reference contraction, but this was significantly (p < 0.05) different from that of the nonpregnant women. CONCLUSIONS: Omental artery segments from nonpregnant, normotensive pregnant and preeclamptic women contract similarly to endothelin-1 and U46619 but exhibit variable responses to norepinephrine and serotonin.

AB - OBJECTIVE: Our purpose was to compare the responsiveness of omental resistance arteries from nonpregnant women and from normotensive and preeclamptic pregnant women to selected contractile agonists. STUDY DESIGN: Omental artery rings with intact endothelium from normotensive premenopausal nonpregnant women and from normal and preeclamptic pregnant women were mounted in Krebs-bicarbonate solution in organ baths for isometric tension recording. After the presence of endothelium was confirmed, cumulative concentrations of norepinephrine, serotonin, U46619, and endothelin-1 were added. Concentration-response curves were constructed and expressed as percentage of a reference 60 mmol/L potassium chloride contraction. Data analysis was by repeated-measures analysis of variance, Newman-Keuls test, and paired or unpaired Student t test, as appropriate. Statistical significance was by two-tailed p < 0.05. RESULTS: Endothelin-1 and U46619 increased tension similarly in all three groups. Norepinephrine increased tension in nonpregnant vessels to a greater extent than in either preeclamptic or pregnant vessels (nonpregnant 114.3 ± 5.42% vs pregnant 65.2% ± 10.5%, P < 0.05). Nonpregnant omental artery developed significantly greater tension than did pregnant tissue at three concentrations of norepinephrine (10-5 mol/L, 3 x 10-5 mol/L, 10-4 mol/L), and preeclamptic vessels developed more tension than that from normal pregnant vessels at 3 x 10-6 mol/L (p = 0.06) and 10-3 mol/L (p < 0.05). There was a negligible change in tension with increasing concentrations of serotonin in the vessels from nonpregnant women; serotonin-induced contraction in the omental arteries from normotensive pregnant women and preeclamptic patients was <6% of the potassium chloride reference contraction, but this was significantly (p < 0.05) different from that of the nonpregnant women. CONCLUSIONS: Omental artery segments from nonpregnant, normotensive pregnant and preeclamptic women contract similarly to endothelin-1 and U46619 but exhibit variable responses to norepinephrine and serotonin.

KW - omental artery

KW - preeclampsia

KW - Vasoconstrictor agonist

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